2023
DOI: 10.3389/fmicb.2023.1276241
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Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Eman M. Khalaf,
Anuja Shrestha,
Michelle Reid
et al.

Abstract: Pollen is a vector for diversification, fitness-selection, and transmission of plant genetic material. The extent to which the pollen microbiome may contribute to host diversification is largely unknown, because pollen microbiome diversity within a plant species has not been reported, and studies have been limited to conventional short-read 16S rRNA gene sequencing (e.g., V4-MiSeq) which suffers from poor taxonomic resolution. Here we report the pollen microbiomes of 16 primitive and traditional accessions of … Show more

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Cited by 2 publications
(2 citation statements)
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“…K. intermedia , a member of the pan-American core maize pollen microbiome ( Khalaf et al., 2023 ), was previously shown to antagonize the fungal pathogen Rhizoctonia solani ( Anak et al., 2021 ), and the bacterial pathogen Pseudomonas syringae in kiwifruit ( Tontou et al., 2016 ), but we could not find any reports from the literature of this species having anti- Fg or anti- Fusarium activities. It is also noteworthy that several studies reported that K. intermedia exhibited plant growth-promoting traits ( Vazquez et al., 2000 ; Pallavi and Gupta, 2013 ; Boechat and Carlos, 2020 ; Pratiwi et al., 2020 ).…”
Section: Discussionmentioning
confidence: 64%
See 1 more Smart Citation
“…K. intermedia , a member of the pan-American core maize pollen microbiome ( Khalaf et al., 2023 ), was previously shown to antagonize the fungal pathogen Rhizoctonia solani ( Anak et al., 2021 ), and the bacterial pathogen Pseudomonas syringae in kiwifruit ( Tontou et al., 2016 ), but we could not find any reports from the literature of this species having anti- Fg or anti- Fusarium activities. It is also noteworthy that several studies reported that K. intermedia exhibited plant growth-promoting traits ( Vazquez et al., 2000 ; Pallavi and Gupta, 2013 ; Boechat and Carlos, 2020 ; Pratiwi et al., 2020 ).…”
Section: Discussionmentioning
confidence: 64%
“…Third, greenhouse trials and confocal microscopy provided direct evidence that a subset of these bacteria could defend the style (silks) and/or the resulting seed progeny against anti-GER Fusarium species and their mycotoxins ( Figures 6 – 9 ; Table 3 ). The pollen-associated anti- Fusarium strain used in confocal microscopy (AS541) was assigned to the species Kluyvera intermedia (based on 16S sequencing), which was recently shown by our group, in a parallel PacBio-based microbiome study ( Khalaf et al., 2023 ), to be present in pollen of 15/17 diverse maize accessions. Finally, full-length 16S rRNA-based taxonomic profiling of pollen-associated bacteria showed that the majority of taxa from the major wild ancestor of modern maize were present in at least one post-domestication landrace in the study panel ( Figure 4 ).…”
Section: Discussionmentioning
confidence: 99%