Consequences of the natural retinoid/retinoid X receptor ligands action in human breast cancer MDA-MB-231 cell line: Focus on functional proteomics

Flodrová, Dana; Toporová, Lucia; Laštovičková, Markéta; Macejova, Dana; Hunáková, Ľuba; Brtko, Július; Bobáľová, Janette

doi:10.1016/j.toxlet.2017.09.001

Cited by 8 publications

(11 citation statements)

References 48 publications

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“…4B). This is in agreement with our previous results reporting the same impact of ATRA (RAR agonist) and 9-cis retinoic acid (9cRA) (RXR agonist) (Flodrova et al 2017).…”

Section: Monitoring Of Quantitative Changes In Selected Tumor Associasupporting

confidence: 94%

“…Obtained 2D qualitative profiles of individual treated samples correspond to our previous results where the effect of natural retinoids, namely ATRA, 9cRA and their combination was monitored (Flodrova et al 2017). Similarly, there are considerable changes in quantitative representation of several proteins.…”

Section: Proteomic Analysis Of Differently Treated Cellssupporting

confidence: 83%

“…ANXA5 showed also decreased tendency after triorganotin chlorides (TBT-Cl, TPT-Cl) treatment, as well as the enhanced effect with ATRA combination. On the other hand, in our previous study (Flodrova et al 2017); there was no evidence of an increased effect of ATRA and 9cRA. Moreover, after treatment by this natural retinoid mixture, the suppression of ANXA5 was insignificant.…”

Section: Monitoring Of Quantitative Changes In Selected Tumor Associacontrasting

confidence: 65%

“…In our previous study, the combined treatments of MDA-MB-231 with ATRA and 9cRA, natural ligand of RXRs, were applied to investigate their possible synergistic action. Several proteins, potentially affected by both RXR/RAR ligands, were clearly demonstrated (Flodrova et al 2017). Accordingly, we have selected proteins that were found to be markedly influenced by treatment with ATRA, triorganotins and their combination and have been associated with cell proliferation, tumor progression and invasiveness, or with cancer suppression.…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, based on our previous work (Flodrova et al 2017), we focused on specific proteins associated with tumor process, namely annexin A5 (ANXA5), vimentin (VIME), glyceraldehyde-3-phosphate dehydrogenase (G3P) and nucleoside diphosphate kinase B (NDKB) (Fig. 3).…”

Section: Monitoring Of Quantitative Changes In Selected Tumor Associamentioning

confidence: 99%

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Novel insights into the combined effect of triorganotin compounds and all-trans retinoic acid on expression of selected proteins associated with tumor progression in breast cancer cell line MDA-MB-231: Proteomic approach

Strouhalová

Toporová²,

Laštovičková³

et al. 2019

gpb

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Trialkyltins and triaryltins function as nuclear retinoid X receptors (RXR) agonists due to their affinity to the ligand-binding domain of RXR subtypes and function as transcriptional activators. We present the data on combined effects of all-trans retinoic acid (ATRA), retinoic acid receptor (RAR) ligand and tributyltin chloride or triphenyltin chloride (RXR ligands) on protein pattern in MDA-MB-231 cells. Proteomic strategies based on bottom-up method were applied in this study. The total cell proteins were extracted, separated on 2D SDS-PAGE and their characterization was achieved by MALDI-TOF/TOF MS/MS. By employing PDQuest™ software, we identified more than 30 proteins differently affected by the above compounds. For further studies, we selected specific proteins associated either with metabolic pathway (glyceraldehyde-3-phosphate dehydrogenase) or to cellular processes as apoptosis, regulation of gene transcription or epithelial-mesenchymal transition (annexin 5, nucleoside diphosphate kinase B and vimentin). We have found that treatment of MDA-MB-231 cells with triorganotins reduced the expression of studied proteins. Moreover, the treatment of MDA-MB-231 cells with triorganotin compounds together with ATRA resulted in an additional reduction of annexin 5, vimentin and nucleoside diphosphate kinase B. These results demonstrate that RXR/RAR heterodimer may act under this experimental design as permissive heterodimer allowing activation of RXR by triorganotins.

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“…4B). This is in agreement with our previous results reporting the same impact of ATRA (RAR agonist) and 9-cis retinoic acid (9cRA) (RXR agonist) (Flodrova et al 2017).…”

Section: Monitoring Of Quantitative Changes In Selected Tumor Associasupporting

confidence: 94%

Section: Proteomic Analysis Of Differently Treated Cellssupporting

confidence: 83%

Section: Monitoring Of Quantitative Changes In Selected Tumor Associacontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Monitoring Of Quantitative Changes In Selected Tumor Associamentioning

confidence: 99%

See 3 more Smart Citations

Novel insights into the combined effect of triorganotin compounds and all-trans retinoic acid on expression of selected proteins associated with tumor progression in breast cancer cell line MDA-MB-231: Proteomic approach

Strouhalová

Toporová²,

Laštovičková³

et al. 2019

gpb

Self Cite

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The effect of all‐trans retinoic acid on the mitochondrial function and survival of cardiomyoblasts exposed to local photodamage

Kureková

Tomaskova

Andelova

et al. 2022

Cell Biology International

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Using H9C2 cardiomyoblasts, we have shown that all-trans retinoic acid (ATRA), the biologically active metabolite of vitamin A, affects mitochondrial dynamics and functions. The low dose (10 nM) ATRA stimulates the expression of nuclear retinoid receptors and induces mechanisms that are protective against severe local damage caused by laser irradiation at the mitochondrial level. These changes include increased density of the mitochondrial network, higher number of mitochondrial junctions, and enhanced mitochondrial velocity. Moreover, the treated cells had lower basal level of reactive oxygen species (ROS) and could maintain mitochondrial potential (ΔΨ m ) after photodamage. Cells treated with 10 nM ATRA had significantly better survival rate after photodamage in comparison to control cells. Cells treated with pharmacological concentration of ATRA (1 µM) expressed higher mitochondrial connectivity without increased motility, which did not lead to better survival or decreased ROS level as was in the case of low-dose ATRA. The proteomics analysis showed changes in proteins related to cellular metabolism (glycolysis) and respiration in ATRA-treated cells. The L-lactate assay confirmed the shift to anaerobic glycolysis in cells treated with 1 µm ATRA, whereas the 10 nM ATRA decreased the level of lactate in medium. The increased levels of cytochrome c or peroxiredoxins 5 level and also lower expression of retinoid and rexinoid receptors were observed in cells treated with 1 µM ATRA. The effect of ATRA is concentration-dependent; the increased mitochondrial dynamics and slower metabolism at 10 nM ATRA contributed significantly to the chance of survival of the cells after photodamage whereas the higher concentration of ATRA overrode the protective effect and led to the unfavorable ones.

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Natural modulators of nonalcoholic fatty liver disease: Mode of action analysis and in silico ADME-Tox prediction

Sharif

Alov

Vitcheva

et al. 2017

Toxicology and Applied Pharmacology

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Consequences of the natural retinoid/retinoid X receptor ligands action in human breast cancer MDA-MB-231 cell line: Focus on functional proteomics

Cited by 8 publications

References 48 publications

Novel insights into the combined effect of triorganotin compounds and all-trans retinoic acid on expression of selected proteins associated with tumor progression in breast cancer cell line MDA-MB-231: Proteomic approach

Novel insights into the combined effect of triorganotin compounds and all-trans retinoic acid on expression of selected proteins associated with tumor progression in breast cancer cell line MDA-MB-231: Proteomic approach

The effect of all‐trans retinoic acid on the mitochondrial function and survival of cardiomyoblasts exposed to local photodamage

Natural modulators of nonalcoholic fatty liver disease: Mode of action analysis and in silico ADME-Tox prediction

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