Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide

Filipović, Miloš R.; Stanić, Dragana; Raicevic, Smiljana; Spasić, Mihajlo; Niketić, Vesna

doi:10.1080/10715760600944296

Cited by 49 publications

(42 citation statements)

References 49 publications

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“…Recently it was shown that upon sustained aerobic exposure to NO., MnSOD-derived NO -initiates the formation of ONOO -which leads to ONOO -decomposition and ONOO -dependent enzyme tyrosine residue nitration, oxidation and partial MnSOD enzyme inactivation. 40 As in our experiments aloin decreased iNOS activity and consequently NO. production, such conditions may also be responsible for the increased activity of MnSOD compared to controls.…”

Section: Discussionsupporting

confidence: 49%

Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells

Nićiforović¹,

Adžić²,

Spasić³

et al. 2007

Cancer Biology & Therapy

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The antiproliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC 50 = 97 mM), caused cell cycle arrest in the S phase, and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mM, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H 2 O 2 . Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.

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Section: Discussionsupporting

confidence: 49%

Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells

Nićiforović¹,

Adžić²,

Spasić³

et al. 2007

Cancer Biology & Therapy

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“…Activity for MnSOD is significantly reduced in EAD brain and CSF compared with the age-matched control, which is consistent with the concept of mitochondrial dysfunction as a factor in the progression of AD. MnSOD was also found to be nitrated and subsequently inactivated in mice by peroxynitrite (121,149). Overexpression of SOD2 increases Ab degradation, while partial deficiency promotes Ab deposition, thereby likely contributing to cognitive decline observed in a transgenic mouse model of AD (134).…”

Section: Ead Carbonylated Proteinsmentioning

confidence: 99%

Redox Proteomics in Selected Neurodegenerative Disorders: From Its Infancy to Future Applications

Butterfield

Perluigi

Reed

et al. 2012

Antioxidants & Redox Signaling

156

142

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Several studies demonstrated that oxidative damage is a characteristic feature of many neurodegenerative diseases. The accumulation of oxidatively modified proteins may disrupt cellular functions by affecting protein expression, protein turnover, cell signaling, and induction of apoptosis and necrosis, suggesting that protein oxidation could have both physiological and pathological significance. For nearly two decades, our laboratory focused particular attention on studying oxidative damage of proteins and how their chemical modifications induced by reactive oxygen species/reactive nitrogen species correlate with pathology, biochemical alterations, and clinical presentations of Alzheimer's disease. This comprehensive article outlines basic knowledge of oxidative modification of proteins and lipids, followed by the principles of redox proteomics analysis, which also involve recent advances of mass spectrometry technology, and its application to selected age-related neurodegenerative diseases. Redox proteomics results obtained in different diseases and animal models thereof may provide new insights into the main mechanisms involved in the pathogenesis and progression of oxidativestress-related neurodegenerative disorders. Redox proteomics can be considered a multifaceted approach that has the potential to provide insights into the molecular mechanisms of a disease, to find disease markers, as well as to identify potential targets for drug therapy. Considering the importance of a better understanding of the cause/effect of protein dysfunction in the pathogenesis and progression of neurodegenerative disorders, this article provides an overview of the intrinsic power of the redox proteomics approach together with the most significant results obtained by our laboratory and others during almost 10 years of research on neurodegenerative disorders since we initiated the field of redox proteomics. Antioxid. Redox Signal. 17, 1610-1655.

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“…Figure 3 shows the rapid increase in detectable signal from an NO-specific electrode when Cu,Zn SOD is incubated with Angeli's salt. Conversely, MnSOD, which forms a stable nitrosyl complex (46), releases NO much more slowly.…”

Section: Fig 3 Reactivity Of Hno With Cuzn Sod and Mnsodmentioning

confidence: 99%

The Specificity of Nitroxyl Chemistry Is Unique Among Nitrogen Oxides in Biological Systems

Flores-Santana

Salmon

Donzelli

et al. 2011

Antioxidants & Redox Signaling

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The importance of nitric oxide in mammalian physiology has been known for nearly 30 years. Similar attention for other nitrogen oxides such as nitroxyl (HNO) has been more recent. While there has been speculation as to the biosynthesis of HNO, its pharmacological benefits have been demonstrated in several pathophysiological settings such as cardiovascular disorders, cancer, and alcoholism. The chemical biology of HNO has been identified as related to, but unique from, that of its redox congener nitric oxide. A summary of these findings as well as a discussion of possible endogenous sources of HNO is presented in this review.

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Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide

Cited by 49 publications

References 49 publications

Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells

Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells

Redox Proteomics in Selected Neurodegenerative Disorders: From Its Infancy to Future Applications

The Specificity of Nitroxyl Chemistry Is Unique Among Nitrogen Oxides in Biological Systems

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