Consequences of Androctonus mauretanicus and Buthus occitanus scorpion venoms on electrolyte levels in rabbits

Daoudi, Khadija; Chgoury, Fatima; Rezzak, Myriam; Bourouah, Oussama; Boussadda, Lotfi; Soukri, Abdelaziz; Sabatier, Jean‐Marc; Oukkache, Naoual

doi:10.1016/j.heliyon.2016.e00221

Cited by 9 publications

(7 citation statements)

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“…Our results are in agreement with those reported previously, 22 where the LD 50 was 290 and 855 μg/kg body weight for Am and Bo , respectively, using IP injection in mice. Daoudi et al 23 reported that the Am venom had a higher lethal potency (180 μg/kg body weight) compared to Bo venom (310 μg/kg body weight). The Am venom showed an almost similar toxicity to that of the Androctonus australis hector venom (335 μg/kg body weight) via IP route.…”

Section: Discussionmentioning

confidence: 99%

Induced pathophysiological alterations by the venoms of the most dangerous Moroccan scorpions Androctonus mauretanicus and Buthus occitanus: A comparative pathophysiological and toxic-symptoms study

et al. 2022

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Scorpion envenomation is a serious public health issue. Androctonus mauretanicus ( Am) and Buthus occitanus ( Bo) are the most dangerous scorpions in Morocco. Despite their medical relevance, no study has yet related their kinetics of symptom apparition and the consequent tissue disorders at the same interval post-injection. This work achieved the first comparative pathophysiological and toxic-symptoms study between the Am and Bo venoms from a biochemical, toxicological and physiopathological standpoint. The activity of venoms and their subletal dose were determined by administration of increasing concentrations of the venoms. 30, 60 and 120 min following the experimental envenomation in mice, the profile of clinical symptoms was underlined and the main organs: brain, heart, lungs, liver and kidneys were removed for histological examination. The Am venom is a rich source of proteins and three-times more toxic than the Bo. The most observed clinical symptoms are neurological and cardiopulmonary. The Am venom caused histopathological alterations at 30, 60, and 120 min which were more important than the Bo. This study highlighted that both venoms exhibited a strong toxicity with variable intensities. Moreover, we showed the presence of correlation between the level of histopathological disorders observed and the intensity of signs appeared at the same time following venom inoculation.

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Section: Discussionmentioning

confidence: 99%

Induced pathophysiological alterations by the venoms of the most dangerous Moroccan scorpions Androctonus mauretanicus and Buthus occitanus: A comparative pathophysiological and toxic-symptoms study

et al. 2022

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“…Among these species, the yellow scorpion Buthus occitanus (B.occitanus) seems to be one of the most dangerous scorpions, on account of its toxic venom causing the majority of envenomation cases [9]. Although several studies had been carried out on this venom [10–13], no study has yet focused on the proteomic composition of the Moroccan B. occitanus scorpion venom despite its medical importance. Moreover, there are various strategies to screen scorpion venoms, from using conventional strategies for targeting one single toxin, to applying the most throughput equipment of screening for a detailed view of all toxic components.…”

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confidence: 99%

Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

et al. 2021

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Buthus occitanus (B.occitanus) is one of the most dangerous scorpions in the world. Despite the involvement of B.occitanus scorpion in severe cases of envenomation in Morocco, no study has focused yet on the proteomic composition of the Moroccan B.occitanus scorpion venom. Mass spectrometry-based proteomic techniques are commonly used in the study of scorpion venoms. The implementation of top-down and bottom-up approaches for proteomic analyses facilitates screening by allowing a global view of the structural aspects of such complex matrices. Here, we provide a partial overview of the venom of B.occitanus, in order to explore the diversity of its toxins and hereafter understand their effects. To this end, a combination of top-down and bottomup approaches was applied using nano-high liquid chromatography coupled to nano-electrospray tandem mass spectrometry (nano-LC-ESI-MS/MS). TheLC-MS results showed that B.occitanus venom contains around 200 molecular masses ranging from 1868 to 16,720 Da, the most representative of which are those between 5000 and 8000 Da. Interestingly, combined top-down and bottom-up LC-MS/MS results allowed the identification of several toxins, which were mainly those acting on ion channels, including those targeting sodium (NaScTxs), potassium (KScTxs), chloride (ClScTxs) and calcium channels (CaScTx), as well as antimicrobial peptides (AMPs), amphipathic peptides, myotropic neuropeptides and hypothetical secreted proteins. This study reveals the molecular diversity of B.occitanus scorpion venom and identifies components that may have useful pharmacological activities.

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“…Venom's neurotoxic peptides target ion channels. Toxins alter the Na + and K + channel membrane conductance, which disrupts the neuronal in ux and results in physio pathological diseases [22].…”

Section: Discussionmentioning

confidence: 99%

The correlation between interleukin 1β (IL-1β) as an inflammatory marker and Malondialdehyde (MDA) as a lipid peroxidation marker and the development of cardiac and pancreatic complications in humans suspected to scorpion poisoning

Mebed,

zakaria,

setouhi

et al. 2023

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Back ground Scorpion envenomation is a common health problem all over the world. Recent experimental studies on animals were held to prove the correlation between interleukin 1 β (IL-1β), lipid peroxidation and nitric oxide (NO) production in the development of cardiac and pancreatic abnormalities after scorpion venom exposure. The aim of this study is to assess the correlation of lipid peroxidation and IL-1β release and the development of cardiac and pancreatic complications associated with scorpion envenomation in humans which will subsequently evaluate their role in prediction of cardiac or pancreatic complications and help the physicians when to determine to use antioxidants and (IL-1β) receptor blockers in treatment in addition to scorpion antivenom. Methods In this study, 88 subjects were recruited from El-Minia university hospital. All the patients admitted with scorpion envenomtion diagnosis were enrolled over a period of 12 months; from the 1st of Jan 2021 till the 30th of Jan 2022. After exclusion of 7 patients, the remaining subjects were divided into four groups of subjects according to their cardiac function into two groups: group A with normal cardiac function contained: 30 normal subjects, group B contained 51 subjects who had abnormal cardiac functions. The patients were considered to have abnormal cardiac function if they had at least one or more abnormal cardiac investigations (cardiac troponin I (c TN-I), electrocardiogram (ECG), and/or, trans thoracic echocardiography (TTE)).The same patients were divided into another two groups: group C with normal pancreatic function contained: 36 normal subjects, group D contained 45 subjects who had abnormal pancreatic function. Pancreatic function was considered abnormal if one or both of serum amylase & random blood sugar results weren’t normal. Results Our results showed the following: Regarding cardiac abnormality, there were significant statistical differences with both levels of IL-1β and MDA. Regarding pancreatic abnormality, there were insignificant statistical differences with both levels of IL-1β and MDA. Cutoff values were calculated to help health care providers to assess when to introduce such treatments in addition to antivenom to help to counteract these complications and decline the mortality rates. Conclusion It was concluded that IL-1β and MDA have significant correlations in development of cardiac and pancreatic functions abnormalities; the treatments that modify their release or counteract their action may be beneficial. Trial registration The research was approved by the ethical committee board of the faculty of medicine, Minia University, with approval number 206: December/2021. Patients or their first degree relatives (according to the conscious level) were informed by all procedures included in the study and were asked to sign informed consent if they agreed to participate in.

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Consequences of Androctonus mauretanicus and Buthus occitanus scorpion venoms on electrolyte levels in rabbits

Cited by 9 publications

References 32 publications

Induced pathophysiological alterations by the venoms of the most dangerous Moroccan scorpions Androctonus mauretanicus and Buthus occitanus: A comparative pathophysiological and toxic-symptoms study

Induced pathophysiological alterations by the venoms of the most dangerous Moroccan scorpions Androctonus mauretanicus and Buthus occitanus: A comparative pathophysiological and toxic-symptoms study

Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

The correlation between interleukin 1β (IL-1β) as an inflammatory marker and Malondialdehyde (MDA) as a lipid peroxidation marker and the development of cardiac and pancreatic complications in humans suspected to scorpion poisoning

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