Consequences of adding gum Arabic as a cryoprotectant on motility and viability of frozen stallion semen

Ali, Mohamed; Musa, Musa; Alfadul, Sulaiman M.; Al-Sobayel, K.

doi:10.1016/j.cryobiol.2017.09.008

Cited by 8 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This research showed that maintaining the sperm motility and progressive motile sperm during cooling and freezing temperature could be achieved more favorably in the diluents containing GA than the diluent containing EY for buck semen. However, this study agrees with the study using GA in cryopreservation of stallion semen (Mohamed Ali et al 2017). Gum Arabic is complex, comprised of polysaccharide, and a branch polymer consisting of galactose, rhamnose, arabinose, and glucuronic acid (Montenegro, et al 2012).…”

Section: Resultssupporting

confidence: 90%

“…The ultimate explanation for this impediment of fertility is the high viscosity negatively affected the sperm survival before or during storage in liquid nitrogen. It has been established that the viscosity of the extender affects the pattern of sperm motion by the addition of Ficoll, carboxy methyl cellulose (CMC), methylcellulose, EY or GA (Amann and Hammerstedt, 1980, Hirai et al 1997, Mohamed Ali et al 2017). In the present Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen study, addition of GA could also alter the movement pattern of Buck spermatozoa.…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, GA is used as cryoprotectant to preserve buck sperm at low temperature. According to study by Mohamed Ali et al (2017), a highly quality of post thawed frozen stallion semen was achieved after freezing, when using GA instead of the EY. However, this present study is considered the comparative research related to GA as an alternative to EY for the cryopreservation of buck spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen

Ali

2021

IJAR

Self Cite

View full text Add to dashboard Cite

Background: Egg yolk (EY) is well known to be toxic for buck spermatozoa, which creates restrictions of its use in cryopreservation. Therefore, this study is to compare the effect of different levels of gum Arabic (GA) in an extender on quality and fertility of cryopreserved buck sperm. Methods: Each ejaculate of six bucks was frozen in Tris with one of concentrations of GA which contained 3, 6, 9 and 12 gm/ 100 ml in place of the EY. Control was Tris extender containing 2.5% of EY. Result: A percentages of total motile sperm (54.92%; P less than 0.05) and progressively motile sperm (26.22%; P less than 0.05) of semen was frozen in Tris containing 9% of GA. Similar to control group, the pregnancy rate of does inseminated with extender containing 9% (50.0%) were significantly higher than those of does inseminated with extender containing 6% (8.33%), 3% (0.0%) and 12% (0.0%). Semen evaluation and fertility rate were similar when replacing the EY with GA in the Tris cryodiluent, after cryopreservation of buck semen. The present study shows that high motility rate of frozen semen and acceptable pregnancy rate can be obtained when using GA in place of EY for cryopreserving the buck sperm.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen

Ali

2021

IJAR

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, a well-defined and pathogen-free substitute of non-animal origin cryoprotectant, i.e. GA was recently tested in stallion' semen extender (Ali et al, 2017). It has been suggested that replacing egg yolk as the nonpermeable cryoprotectant in extenders with a new substance like Gum Arabic (GA) may improve the physical traits of semen and subsequent fertility of ram semen used in artificial insemination in ewes.…”

Section: Introductionmentioning

confidence: 99%

Privilege of Gum Arabic inclusion in semen extender compared with egg yolk on the Herri ram’s subsequent fertility outcomes

Zeitoun¹

2017

IJAR

Self Cite

View full text Add to dashboard Cite

This experiment was designed to scrutinize effects of inclusion of Gum Arabic versus egg yolk in either fresh or frozen extender for Herri ram' semen on subsequent pregnancy rate. Semen ejaculates were collected from two rams. Two trials were attempted. Trial I, semen ejaculates were diluted with freshly prepared extenders either contained 10% EY (Control) or 6% GA (GA). Ewes were randomly selected and subdivided into 2 groups and inseminated. Trial II, extenders were frozen (-20°C) for 30 days before using, thawed and semen ejaculates from same rams were diluted and used in inseminations. Ewes were subdivided into 2 groups and inseminated with semen diluted in either control or GA6 extender. Results revealed that using fresh extender surpassed frozen one in achieving better pregnancy of the artificial insemination. Pregnancy rate derived of inseminations prepared by GA6 extender was significantly higher (P<0.0) than control extender. Therefore, using 6% of Gum Arabic in fresh extender can ultimately improve the fertility rate in Herri sheep.

show abstract

Effects of resveratrol on DLD and NDUFB9 decrease in frozen semen of Mongolian sheep

Chen,

Meng,

Liu

et al. 2024

Cryobiology

View full text Add to dashboard Cite

Consequences of adding gum Arabic as a cryoprotectant on motility and viability of frozen stallion semen

Cited by 8 publications

References 26 publications

Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen

Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen

Privilege of Gum Arabic inclusion in semen extender compared with egg yolk on the Herri ram’s subsequent fertility outcomes

Effects of resveratrol on DLD and NDUFB9 decrease in frozen semen of Mongolian sheep

Contact Info

Product

Resources

About