Consequences of acclimation on the resistance to acute thermal stress: Proteomic focus on mussels from pristine site

Péden, Romain; Rocher, Béatrice; Chan, Philippe; Vaudry, David; Poret, Agnès; Olivier, Stéphanie; Foll, Frank Le; Bultelle, Florence

doi:10.1016/j.marenvres.2016.02.006

Cited by 7 publications

(8 citation statements)

References 58 publications

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“…Protein extraction and two-dimensional analysis were performed as previously described in Péden et al, 2016. Gills of mussels sacrificed at day 28 (two conditions: group T28-control and group T28-test, n=8) were ground using Precellys ® Homogenizers and sonicated in a 1:4 ratio of lysis buffer (9 M urea, 2% CHAPS, 65 mM dithioerythritol, 0.02% pharmalyte 3-10NL, GE Healthcare and 16 μg•mL −1 aprotinin).…”

Section: Proteome Analysis and Statistical Treatmentmentioning

confidence: 99%

“…In ecotoxicology, 2DE has been widely used to study Mytilus species responses to various stresses including temperature, mainly in M. galloprovincialis, M. californianus or M. trossulus (Tomanek and Zuzow, 2010;Ji et al, 2013;Gomes et al, 2014;Xu et al, 2016) but also in M. edulis (Apraiz et al, 2006;Sheehan and McDonagh, 2008;Letendre et al, 2011;Hu et al, 2014;Péden et al, 2016;Péden et al, 2018). We thus conducted a 2DE proteomic analysis of gills collected from mussels maintained at 7.5°C and 20°C.…”

Section: Proteomic Analysis Pinpoints a Marked Recruitment Of Stress Proteinsmentioning

confidence: 99%

“…These genes were chosen to cover different metabolic pathways (energetic metabolism, oxidative stress scavenging, stress response, detoxication, immune response and cell cycle regulation), on the basis of previous studies. Hence, molecular components involved in the process of adaptation and resistance to thermal stress are numerous and have been reviewed in several publications of interest (for examples, please see: Somero, 2010;Lockwood and Somero, 2011;Fields et al, 2012;Tomanek, 2012Tomanek, , 2014Péden et al, 2016;Clark et al, 2017;Coppola et al, 2018;Feidantsis et al, 2020). In addition, we genotyped individuals (Glu-5' locus, Inoue et al;1995;Gerard et al, 2015) to verify whether there is potential inter-individual variability in the response pattern to temperature change.…”

Section: Introductionmentioning

confidence: 99%

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Molecular response of a sub-antarctic population of the blue mussel (Mytilus edulis platensis) to a moderate thermal stress

Bultelle

Boutet

Devin

et al. 2021

Marine Environmental Research

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Section: Proteome Analysis and Statistical Treatmentmentioning

confidence: 99%

Section: Proteomic Analysis Pinpoints a Marked Recruitment Of Stress Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular response of a sub-antarctic population of the blue mussel (Mytilus edulis platensis) to a moderate thermal stress

Bultelle

Boutet

Devin

et al. 2021

Marine Environmental Research

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“…gigas presented higher expression levels of Enolase in Low pH exposure compared to Low pH + As (Table 2). Other studies have described increased expression of Enolase in bivalves, such as Mytilus edulis under thermal stress (Péden et al, 2016) and S. cucculata exposed to TBT (Khondee et al, 2016). Glycolysis stimulation by Enolase has been discussed as a mechanism to increase carbohydrate metabolism in bivalves experiencing energetically demanding conditions (Tomanek, 2014;Artigaud et al, 2015).…”

Section: Protein Changes Under Low Ph + Asmentioning

confidence: 99%

Impacts of the combined exposure to seawater acidification and arsenic on the proteome of Crassostrea angulata and Crassostrea gigas

et al. 2018

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Proteomic analysis was performed to compare the effects of Arsenic (As), seawater acidification (Low pH) and the combination of both stressors (Low pH + As) on Crassostrea angulata and Crassostrea gigas juveniles in the context of global environmental change. This study aimed to elucidate if two closely related Crassostrea species respond similarly to these environmental stressors, considering both single and combined exposures, to infer if the simultaneous exposure to both stressors induced a differentiated response. Identification of the most important differentially expressed proteins between conditions revealed marked differences in the response of each species towards single and combined exposures, evidencing species-related differences towards each experimental condition. Moreover, protein alterations observed in the combined exposure (Low pH + As) were substantially different from those observed in single exposures. Identified proteins and their putative biological functions revealed an array of modes of action in each condition. Among the most important, those involved in cellular structure (Actin, Atlastin, Severin, Gelsolin, Coronin) and extracellular matrix modulation (Ependymin, Tight junction ZO-1, Neprilysin) were strongly regulated, although in different exposure conditions and species. Data also revealed differences regarding metabolic modulation capacity (ATP β, Enolase, Aconitate hydratase) and oxidative stress response (Aldehyde dehydrogenase, Lactoylglutathione, Retinal dehydrogenase) of each species, which also depended on single or combined exposures, illustrating a different response capacity of both oyster species to the presence of multiple stressors. Interestingly, alterations of piRNA abundance in C. angulata suggested genome reconfiguration in response to multiple stressors, likely an important mode of action related to adaptive evolution mechanisms previously unknown to oyster species, which requires further investigation. The present findings provide a deeper insight into the complexity of C. angulata and C. gigas responses to environmental stress at the proteome level, evidencing different capacities to endure abiotic changes, with relevance regarding the ecophysiological fitness of each species and competitive advantages in a changing environment.

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“…However, mRNA expression alone may fail to predict much of the variation in the measurable proteome, and multiple studies have found that circadian mRNA expression poorly corresponds with rhythmic proteins (Hurley et al, 2018; Mauvoisin et al, 2014; Robles et al, 2014). Given that proteins reflect the basic functional molecular phenotype, proteomic studies in intertidal mussels can reveal sensitivity to environmental stress, such as oxidative stress (Tomanek, 2015), hypoxia (Fields et al, 2014), hyposalinity (Tomanek et al, 2012), and high temperatures (Fields et al, 2012; Péden et al, 2016). To understand how their proteome varies naturally in this uniquely dynamic environment, we aimed to characterize the oscillating baseline of protein abundances in the gill tissue of the California mussel ( M. californianus Conrad, 1837) during acclimation to consistent L:D and tidal cycles in a tidal simulator.…”

Section: Introductionmentioning

confidence: 99%

Circadian and circatidal rhythms of protein abundance in the California mussel (Mytilus californianus)

Elowe

Tomanek

2021

Molecular Ecology

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Coastal habitats fluctuate with the 12.4 h tidal and 24 h light/dark cycle to predictably alter conditions such as air exposure, temperature, and food availability. Intertidal sessile bivalves exhibit behavioural and physiological adjustments to minimize the challenges of this environment. We investigated a high‐resolution time course of the changes in protein abundance in the gill tissue of the intertidal mussel Mytilus californianus in a simulated tidal environment of 12:12 h light:dark cycles and a matching 6:6 h high:low tide cycle within each 12 h period. Approximately 38% of detected proteins showed significant rhythms in their abundances, with diversity in the phases of rhythmic isoforms. The circadian rhythm was dominant in protein abundance changes, particularly with oxidative metabolism. A tidal cycle elicited changes within functional groups, including in cytoskeletal proteins, chaperones, and oxidative stress proteins. In addition to protein abundance changes, we found the possibility for post‐translational modifications driving rhythms, including methylation, mitochondrial peptide processing (proteolysis), and acylation. Dynamic changes in the proteome across functional categories demonstrate the importance of the tidal environment in entraining cellular processes, confirming that differential expression studies should not assume a static baseline of cellular conditions in intertidal organisms.

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Consequences of acclimation on the resistance to acute thermal stress: Proteomic focus on mussels from pristine site

Cited by 7 publications

References 58 publications

Molecular response of a sub-antarctic population of the blue mussel (Mytilus edulis platensis) to a moderate thermal stress

Molecular response of a sub-antarctic population of the blue mussel (Mytilus edulis platensis) to a moderate thermal stress

Impacts of the combined exposure to seawater acidification and arsenic on the proteome of Crassostrea angulata and Crassostrea gigas

Circadian and circatidal rhythms of protein abundance in the California mussel (Mytilus californianus)

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