Consensus Receptor-Binding Domain-Targeted Aptamer Selection and Designing of a Photonic Crystal-Decorated Aptasensor for SARS-CoV-2

Murtaza, G.; Rizvi, Aysha Sarfraz; Xue, Min; Qiu, Lili; Meng, Zihui

doi:10.1021/acs.analchem.2c00937

Cited by 24 publications

(23 citation statements)

References 36 publications

(53 reference statements)

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“…Aptamers, as recognition molecules, are key components of hydrogel biosensors, and the degree of their modification will undoubtedly influence the detection performance of the aptasensors 13,22 . Thus, the 2DPCH films prepared from the optimized polymerizable precursor solutions were modified with different concentrations of aptamers (C Aptamer ).…”

Section: Optimization Of the S1/s2-2dpch-110 Aptasensorsmentioning

confidence: 99%

“…Presently, a variety of sensors based on smart 2DPC hydrogels have been developed for detecting different target analytes, such as H 2 O 2 12 , Ag +13, , urea 14 , glucose 15,16 , amino acids 13,17 , proteins 18,19 and microorganisms [20][21][22] . For example, Cai et al 12 .…”

Section: Introductionmentioning

confidence: 99%

“…A urea and urease inhibitor sensor 14 was constructed by introducing urease into a 2DPCH as a recognition element. Recently, aptamer-crosslinked 2DPCH have been reported to detect Ag + , Cys and SARS-Corona viruses 13,22 . Inspired by these works, the design and construction of simple, highly sensitive and selective 2DPCHsensors for detecting proteases in body fluids is promising.…”

Section: Introductionmentioning

confidence: 99%

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Aptamer-functionalized smart photonic hydrogels: application for the detection of thrombin in human serum

Shen

et al. 2022

NPG Asia Mater

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Smart photonic hydrogels based on two-dimensional photonic crystals (2DPC) provide a promising sensing platform for constructing novel chemical and biological sensors due to their facile optical signal readout and highly sensitive responsivity toward target analytes. Aptamers, as recognition elements with high selectivity and affinity, are extensively used to construct a variety of sensors. Herein, we developed two partially base complementary aptamer-functionalized 2DPC hydrogels as aptasensors for the detection of thrombin (TB) in human serum. The photonic hydrogel aptasensors swelled upon exposure to TB solution, leading to an increase in the particle spacing of the 2DPCs. The particle spacing changes were acquired by simply measuring the diameters of the Debye ring diffracted by the 2DPCs without the requirement of sophisticated instruments. The aptasensor swelling resulted from the decrease in the hydrogel cross-linking density induced by the specific binding between one of the aptamers and TB and the increase in hydrogel mixing free energy induced by the introduction of TB. The particle spacing increase of the optimized aptasensor was linear over the TB concentration range of 1–500 nM, and the limit of detection was 0.64 nM. The constructed 2DPC hydrogel aptasensor was used to detect TB in human serum and achieved recoveries of 95.74–104.21% and a relative standard deviation of 2.52–6.58%, showing the practicability and accuracy of the sensor. The aptamer-actuated 2DPC hydrogel biosensor provides a new strategy for designing other target molecule-sensitive aptasensors, showing great potential for development into home kits.

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Section: Optimization Of the S1/s2-2dpch-110 Aptasensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aptamer-functionalized smart photonic hydrogels: application for the detection of thrombin in human serum

Shen

et al. 2022

NPG Asia Mater

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“…For example, Gu’s group [ 27 ] first constructed a Hg 2+ -sensitive three-dimensional inverse opal photonic crystal hydrogel functionalized by an aptamer for colorimetric detection of Hg 2+ . Recently, several aptasensors based on 2DPC hydrogels have been used to achieve the detection of cysteine [ 28 , 29 ], adenosine [ 30 ] and SARS-CoV-2 [ 31 ]. Therefore, it is a promising strategy to construct various sensors via combining aptamers with 2DPC hydrogels, based on the specific binding between aptamers and analytes, Debye diffraction of 2DPC and the volume phase transition of hydrogel, achieving simple, low-cost and selective detection of target analytes.…”

Section: Introductionmentioning

confidence: 99%

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Shen

Shi

et al. 2022

Biosensors

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Responsive two-dimensional photonic crystal (2DPC) hydrogels have been widely used as smart sensing materials for constructing various optical sensors to accurately detect different target analytes. Herein, we report photonic hydrogel aptasensors based on aptamer-functionalized 2DPC poly(acrylamide-acrylic acid-N-tert-butyl acrylamide) hydrogels for facile, label-free and colorimetric detection of lysozyme in human serum. The constructed photonic hydrogel aptasensors undergo shrinkage upon exposure to lysozyme solution through multi-factors cooperative actuation. Here, the specific binding between the aptamer and lysozyme, and the simultaneous interactions between carboxyl anions and N-tert-butyl groups with lysozyme, increase the cross-linking density of the hydrogel, leading to its shrinkage. The aptasensors’ shrinkage decreases the particle spacing of the 2DPC embedded in the hydrogel network. It can be simply monitored by measuring the Debye diffraction ring of the photonic hydrogel aptasensors using a laser pointer and a ruler without needing sophisticated apparatus. The significant shrinkage of the aptasensors can be observed by the naked eye via the hydrogel size and color change. The aptasensors show good sensitivity with a limit of detection of 1.8 nM, high selectivity and anti-interference for the detection of lysozyme. The photonic hydrogel aptasensors have been successfully used to accurately determine the concentration of lysozyme in human serum. Therefore, novel photonic hydrogel aptasensors can be constructed by designing functional monomers and aptamers that can specifically bind target analytes.

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“…Although these devices are portable and economical enough compared to professional laboratory equipment, their cost is not negligible. Color as a visual perception allows for an intuitive visualization method to provide information to users with the naked eye only and is seen as having great potential in the POCT scenarios. − In the previous work of our group, a pressure-based biosensor was proposed that integrated both a pressure sensor for electrical signal output and an electrochromic device for visual detection . While this strategy enabled dual-signal detection of biomolecules, it still relied on a power supply and complicated wiring connections, which limited the size and cost of the device.…”

mentioning

confidence: 99%

Point-of-Care Immunoassay Based on a Multipixel Dual-Channel Pressure Sensor Array with Visual Sensing Capability of Full-Color Switching and Reliable Electrical Signals

Huang

Zeng

et al. 2022

Anal. Chem.

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The point-of-care (POC) method with affordability and portability for the sensitive detection of biological substances is an emerging topic in rapid disease screening and personalized medicine. In this work, we demonstrated a diverse responsive platform based on a dual-channel pressure sensor (DCPS). The DCPS had a multilayer flexible architecture consisting of a photonic hydrogel with chromatic transitions and a piezoresistive pressure sensor as the electrical data transmission unit, both of which had the property of pressure-induced mechanical stimulus feedback. By incorporating a platinum nanoparticles-labeled detection antibody (PtNPs-dAb) into the sandwich-type immunoreaction for the target carcinoembryonic antigen (CEA, as a model analyte), gas decomposition could be triggered by the addition of hydrogen peroxide (H2O2) to induce a significant increase under pressure in a closed chamber. Meanwhile, the DCPS enabled an accurate electrical signal output, and the photonic hydrogel converted spatiotemporal stimuli into eye-readable colorations with string brilliance. In this way, the target concentration could be quantificationally related to the electrical response and intuitively perceived through visible color alterations. Under optimal conditions, a sensitive determination of CEA was performed in a detectable range of 0.3–60 ng/mL with a limit of detection (LOD) of 0.13 ng/mL. In addition, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Furthermore, an array-based immunoassay device was fabricated to conceptually validate its application potential in high-throughput biomedical detection and inspire a dual-signal POC diagnostic platform in a friendly way for resource-limited settings.

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Consensus Receptor-Binding Domain-Targeted Aptamer Selection and Designing of a Photonic Crystal-Decorated Aptasensor for SARS-CoV-2

Cited by 24 publications

References 36 publications

Aptamer-functionalized smart photonic hydrogels: application for the detection of thrombin in human serum

Aptamer-functionalized smart photonic hydrogels: application for the detection of thrombin in human serum

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Point-of-Care Immunoassay Based on a Multipixel Dual-Channel Pressure Sensor Array with Visual Sensing Capability of Full-Color Switching and Reliable Electrical Signals

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