Consensus Guidelines for CSF and Blood Biobanking for CNS Biomarker Studies

Teunissen, Charlotte E.; Tumani, Hayrettin; Bennett, Jeffrey L.; Berven, Frode S.; Brundin, Lou; Comabella, Manuel; Franciotta, Diego; Federiksen, Jette L.; Fleming, John O.; Furlan, Roberto; Hintzen, Rogier Q.; Hughes, Steve; Jiménez, Connie R.; Johnson, Michael H.; Killestein, Joep; Krasulová, Eva; Kühle, Jens; Magnone, Maria Chiara; Petzold, Axel; Rajda, Cecília; Rejdak, Konrad; Schmidt, Hollie; Pesch, Vincent Van; Waubant, Emmanuelle; Wolf, Christian; Deisenhammer, Florian; Giovannoni, Gavin; Hemmer, Bernhard

doi:10.1155/2011/246412

Cited by 50 publications

(81 citation statements)

References 40 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[20] Our data show that it is possible to collect up to 20ml of CSF with every type of needle without increasing the risk of PDPH and the discomfort for the patient.…”

Section: Q and 22s (Tab 1)mentioning

confidence: 67%

The use of the 25 Sprotte needle markedly reduces post-dural puncture headache in routine neurological practice

Bertolotto¹,

Malentacchi²,

Capobianco³

et al. 2015

Cephalalgia

View full text Add to dashboard Cite

Objectives: to test the feasibility of lumbar puncture (LP) using 25 gauge (G) needles in daily neurological practice and to compare the risk of post-dural puncture headache (PDPH) with 4 types of needles. Methods:In a prospective rater-blind study, pros and cons of 4 different LP needles, 20G Quincke (20Q), 22G Sprotte (22S), 25G Whitacre (25W), and 25G Sprotte (25S), were evaluated in 394 LPs performed by 7 neurologists. The neurologist performing the LP recorded type and size of needle, intensity of pain, safety, time of the procedure and failure or success.Between 5 and 15 days later another neurologist, blind for the type of needle used, completed an ad-hoc questionnaire for PDPH.Results: PDPH developed in 35.9% patients when using a 20Q needle, in 12.9%, 6.8% and 1.6% respectively when using a 22S, 25W or 25S needle. The difference in incidence of PDPH following LP performed with 20Q needle and 25S or 22S was statistically significant (p<0.001 and p=0.008 respectively) and it approached significance when comparing 25S and 25W (p=0.06). As 25W and 25S needles need CSF aspiration LP requires more time and skill. Pain caused by LP was similar with the 4 needles. Conclusion:The use of 25S needle in diagnostic LP reduces the frequency and severity of PDPH.

show abstract

“…[20] Our data show that it is possible to collect up to 20ml of CSF with every type of needle without increasing the risk of PDPH and the discomfort for the patient.…”

Section: Q and 22s (Tab 1)mentioning

confidence: 67%

The use of the 25 Sprotte needle markedly reduces post-dural puncture headache in routine neurological practice

Bertolotto¹,

Malentacchi²,

Capobianco³

et al. 2015

Cephalalgia

View full text Add to dashboard Cite

show abstract

“…Plasma was purchased from Bioreclamation, Inc (Westbury, NY) (individual and pooled control human plasma), and PrecisionMed, Inc. (San Diego, CA) (individual controls) and also collected from St. Peter's Hospital (Troy, NY) (individual controls) following consensus guidelines for blood biobanking (Teunissen et al 2011). All participants were recruited with informed consent and approval by the Institutional Review Board.…”

Section: Sample Sourcementioning

confidence: 99%

Increased levels of hyper-stable protein aggregates in plasma of older adults

Xia

Trasatti

Wymer

et al. 2016

AGE

View full text Add to dashboard Cite

Proteins that misfold into hyper-stable/degradation-resistant species during aging may accumulate and disrupt protein homeostasis (i.e., proteostasis), thereby posing a survival risk to any organism. Using the method diagonal two-dimensional (D2D) SDS-PAGE, which separates hyper-stable SDS-resistant proteins at a proteomics level, we analyzed the plasma of healthy young (<30 years) and older (60-80 years) adults. We discovered the presence of soluble SDSresistant protein aggregates in the plasma of older adults, but found significantly lower levels in the plasma of young adults. We identified the inflammation-related chaperone protein haptoglobin as the main component of the hyper-stable aggregates. This observation is consistent with the growing link between accumulations of protein aggregates and aging across many organisms. It is plausible higher amounts of SDS-resistant protein aggregates in the plasma of older adults may reflect a compromise in proteostasis that may potentially indicate cellular aging and/or disease risk. The results of this study have implications for further understanding the link between aging and the accumulation of protein aggregates, as well as potential for the development of aging-related biomarkers. More broadly, this novel application of D2D SDS-PAGE may be used to identify, quantify, and characterize the degradation-resistant protein aggregates in human plasma or any biological system.

show abstract

“…However, it is likely that depletion of high abundance proteins may lead to depletion of other lower abundance proteins through meaningful protein-protein interactions. A series of guidelines have been proposed by Teunissen and colleagues for the collection, handling and storage of CSF for biomarker studies [213]. Contamination of CSF with blood complicates the proteomic analysis since CSF has a lower protein concentration (~1/200) compared to blood.…”

Section: Future Directions In Quantitative Neuroproteomicsmentioning

confidence: 99%

Recent advances in quantitative neuroproteomics

Craft

Chen

Nairn

2013

Methods

122

View full text Add to dashboard Cite

The field of proteomics is undergoing rapid development in a number of different areas including improvements in mass spectrometric platforms, peptide identification algorithms and bioinformatics. In particular, new and/or improved approaches have established robust methods that not only allow for in-depth and accurate peptide and protein identification and modification, but also allow for sensitive measurement of relative or absolute quantitation. These methods are beginning to be applied to the area of neuroproteomics, but the central nervous system poses many specific challenges in terms of quantitative proteomics, given the large number of different neuronal cell types that are intermixed and that exhibit distinct patterns of gene and protein expression. This review highlights the recent advances that have been made in quantitative neuroproteomics, with a focus on work published over the last five years that applies emerging methods to normal brain function as well as to various neuropsychiatric disorders including schizophrenia and drug addiction as well as of neurodegenerative diseases including Parkinson’s disease and Alzheimer’s disease. While older methods such as two-dimensional polyacrylamide electrophoresis continued to be used, a variety of more in-depth MS-based approaches including both label (ICAT, iTRAQ, TMT, SILAC, SILAM), label-free (label-free, MRM, SWATH) and absolute quantification methods, are rapidly being applied to neurobiological investigations of normal and diseased brain tissue as well as of cerebrospinal fluid (CSF). While the biological implications of many of these studies remain to be clearly established, that there is a clear need for standardization of experimental design and data analysis, and that the analysis of protein changes in specific neuronal cell types in the central nervous system remains a serious challenge, it appears that the quality and depth of the more recent quantitative proteomics studies is beginning to shed light on a number of aspects of neuroscience that relates to normal brain function as well as of the changes in protein expression and regulation that occurs in neuropsychiatric and neurodegenerative disorders.

show abstract

Consensus Guidelines for CSF and Blood Biobanking for CNS Biomarker Studies

Cited by 50 publications

References 40 publications

The use of the 25 Sprotte needle markedly reduces post-dural puncture headache in routine neurological practice

The use of the 25 Sprotte needle markedly reduces post-dural puncture headache in routine neurological practice

Increased levels of hyper-stable protein aggregates in plasma of older adults

Recent advances in quantitative neuroproteomics

Contact Info

Product

Resources

About