Consensus genes of the literature to predict breast cancer recurrence

Lauss, Martin; Kriegner, Albert; Vierlinger, Klemens; Visne, Ilhami; Yıldız, Ahmet; Dilaveroglu, Erkan; Noehammer, Christa

doi:10.1007/s10549-007-9716-3

Cited by 27 publications

(21 citation statements)

References 61 publications

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“…Consistent with this, NuSAP was detected in four independent studies aiming to identify genes related to clinical outcome of breast cancer (Lauss et al, 2008). Thus, apart from being a reliable proliferation marker, NuSAP holds the potential of becoming a diagnostic marker for breast cancer and potentially also other tumor types.…”

Section: Discussionsupporting

confidence: 53%

NuSAP is essential for chromatin-induced spindle formation during early embryogenesis

et al. 2010

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SummaryMitotic spindle assembly is mediated by two processes: a centrosomal and a chromosomal pathway. RanGTP regulates the latter process by releasing microtubule-associated proteins from inhibitory complexes. NuSAP, a microtubule-and DNA-binding protein, is a target of RanGTP and promotes the formation of microtubules near chromosomes. However, the contribution of NuSAP to cell proliferation in vivo is unknown. Here, we demonstrate that the expression of NuSAP highly correlates with cell proliferation during embryogenesis and adult life, making it a reliable marker of proliferating cells. Additionally, we show that NuSAP deficiency in mice leads to early embryonic lethality. Spindle assembly in NuSAP-deficient cells is highly inefficient and chromosomes remain dispersed in the mitotic cytoplasm. As a result of sustained spindle checkpoint activity, the cells are unable to progress through mitosis, eventually leading to caspase activation and apoptotic cell death. Together, our findings demonstrate that NuSAP is essential for proliferation of embryonic cells and, simultaneously, they underscore the importance of chromatin-induced spindle assembly.

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Section: Discussionsupporting

confidence: 53%

NuSAP is essential for chromatin-induced spindle formation during early embryogenesis

et al. 2010

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“…High levels of B-MYB can predict the probability of breast cancer recurrence (33,35). B-MYB is also included in a set of 374 genes that were extracted from 44 published gene lists and are significantly associated with breast cancer recurrence (36). Furthermore, B-MYB is one of the marker genes of a commercially marketed 21-gene assay that can predict recurrence in estrogen receptorpositive, node-negative patients (37).…”

Section: Resultsmentioning

confidence: 99%

B-MYB Is Required for Recovery from the DNA Damage–Induced G2 Checkpoint in p53 Mutant Cells

2009

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In response to DNA damage, several signaling pathways that arrest the cell cycle in G(1) and G(2) are activated. The down-regulation of mitotic genes contributes to the stable maintenance of the G(2) arrest. The human LINC or DREAM complex, together with the B-MYB transcription factor, plays an essential role in the expression of G(2)-M genes. Here, we show that DNA damage results in the p53-dependent binding of p130 and E2F4 to LINC and the dissociation of B-MYB from LINC. We find that B-MYB fails to dissociate from LINC in p53 mutant cells, that this contributes to increased G(2)-M gene expression in response to DNA damage in these cells, and, importantly, that B-MYB is required for recovery from the G(2) DNA damage checkpoint in p53-negative cells. Reanalysis of microarray expression data sets revealed that high levels of B-MYB correlate with a p53 mutant status and an advanced tumor stage in primary human breast cancer. Taken together, these data suggest that B-MYB/LINC plays an important role in the DNA damage response downstream of p53.

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“…A total of 28 bladder cancer gene signatures from 14 studies (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), as well as 11 nonbladder cancer-derived gene signatures devised for various purposes (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32) were extracted from the literature (Supplementary Table S1). GeneSymbols in the signatures were updated using the official HGNC GeneSymbols downloaded from the HGNC Web site (33).…”

Section: Gene Signaturesmentioning

confidence: 99%

Prediction of Stage, Grade, and Survival in Bladder Cancer Using Genome-wide Expression Data: A Validation Study

Lauss

Ringnér

Höglund

2010

Clinical Cancer Research

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Purpose: To evaluate performances of published gene signatures for the assessment of urothelial carcinoma.Experimental Design: We evaluated 28 published gene signatures designed for diagnostic and prognostic purposes of urothelial cancer. The investigated signatures include eight signatures for stage, five for grade, four for progression, and six for survival. We used two algorithms for classification, nearest centroid classification and support vector machine, and Cox regression to evaluate signature performance in four independent data sets.Results: The overlap of genes among the signatures was low, ranging from 11% among stage signatures to 0.6% among survival signatures. The published signatures predicted muscle-invasive and high-grade tumors with accuracies in the range of 70% to 90%. The performance for a given signature varied considerably with the validation data set used, and interestingly, some of the best performing signatures were not designed for the tested classification problem. In addition, several nonbladder-derived gene signatures performed equally well. Large randomly selected gene signatures performed better than the published signatures, and by systematically increasing signature size, we show that signatures with >150 genes are needed to obtain robust performance in independent validation data sets. None of the published survival signatures performed better than random assignments when applied to independent validation data.Conclusion: We conclude that gene expression signatures with >150 genes predict muscle-invasive growth and high-grade tumors with robust accuracies. Special considerations have to be taken when designing gene signatures for outcome in bladder cancer. Clin Cancer Res; 16(17); 4421-33. ©2010 AACR.

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Consensus genes of the literature to predict breast cancer recurrence

Abstract: The '374 Gene Set' comprises a molecular basis of metastatic breast cancer progression. Starting from this gene set it might be possible to construct a clinically relevant classifier, which then again needs to be validated.

Cited by 27 publications

References 61 publications

NuSAP is essential for chromatin-induced spindle formation during early embryogenesis

NuSAP is essential for chromatin-induced spindle formation during early embryogenesis

B-MYB Is Required for Recovery from the DNA Damage–Induced G2 Checkpoint in p53 Mutant Cells

Prediction of Stage, Grade, and Survival in Bladder Cancer Using Genome-wide Expression Data: A Validation Study

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