Consecutive ultrafiltration and silica adsorption for recovery of extracellular antibiotic resistance genes from an urban river

Liu, Miaomiao; Hata, Akihiko; Katayama, Hiroyuki; Kasuga, Ikuro

doi:10.1016/j.envpol.2020.114062

Cited by 34 publications

(34 citation statements)

References 41 publications

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“…The significantly positive correlation between intI1 and crAssphage in the studied rivers suggests that class 1 integrons and the associated ARGs and MGEs could be originated from human feces (Chen et al, 2019a;Karkman et al, 2019;Agramont et al, 2020;Nguyen et al, 2021). Clear shifts in the relative abundances and profiles were observed after the entry of treated effluent in Tamagawa River, Iruma River, and Arakawa River, which is consistent with the qPCR results in Tamagawa River (Liu et al, 2020). Drastic changes in the microbial community were also observed from upstream to downstream in Iruma River and Arakawa River, suggesting that the microbial community in the effluent could mostly determine the resistome in these rivers.…”

Section: Discussionsupporting

confidence: 86%

“…In Japan, several studies have reported the presence of antimicrobial-resistant E. coli in rivers (Ham et al, 2012;Urase and Sato, 2016;Gomi et al, 2017;Yamashita et al, 2017;Suzuki et al, 2019;Tsutsui and Urase, 2019). Although some studies investigated specific ARGs in rivers (Nguyen et al, 2019;Liu et al, 2020), to the best of our knowledge, no comprehensive profiles of ARGs and MGEs in urban rivers have been reported in Japan. In the present study, HT-qPCR was performed to determine the prevalence of 67 ARGs and MGEs in 24 urban rivers in Tokyo and surrounding prefectures.…”

Section: Introductionmentioning

confidence: 99%

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High-Throughput Screening of Antimicrobial Resistance Genes and Their Association With Class 1 Integrons in Urban Rivers in Japan

Kasuga

Nagasawa

Suzuki

et al. 2022

Front. Environ. Sci.

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Antimicrobial resistance (AMR) is a serious public health concern. Many countries have implemented AMR surveillance programs for humans and animals, but a scheme for monitoring AMR in the environment has not yet been established. Class 1 integrons, which can acquire antimicrobial resistance genes (ARGs) to gene cassettes, were proposed as a candidate to evaluate the anthropogenic impacts on AMR. However, the association between class 1 integrons and ARGs in aquatic environments is less studied and requires further elucidation. This study used high-throughput quantitative polymerase chain reaction (HT-qPCR) to characterize the pollution profiles of ARGs and mobile gene elements (MGEs) in 24 urban rivers in Tokyo and its surrounding area. The abundance of class 1 integron-integrase gene (intI1) and the array of class 1 integron gene cassettes were also determined. In total, 9–53 target genes were detected per sample, and their abundances increased following effluent discharge from wastewater treatment plants. The river and wastewater samples were categorized based on their HT-qPCR profiles, indicating that this method was useful for characterizing the pollution status in aquatic environments. The prevalence of intI1 in the rivers was observed. Some ARGs and MGEs were positively correlated with intI1, indicating that intI1 could be used as a proxy for monitoring these ARGs and MGEs in urban rivers. Long-read sequencing of class 1 integron gene cassettes revealed that one to three ARGs were present in the gene cassettes. Regardless of the sample type, blaGES-24, aadA2, and qacH were dominant in the gene cassettes. The source and spread of class 1 integrons carrying these ARGs in aquatic environments should be further monitored.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

High-Throughput Screening of Antimicrobial Resistance Genes and Their Association With Class 1 Integrons in Urban Rivers in Japan

Kasuga

Nagasawa

Suzuki

et al. 2022

Front. Environ. Sci.

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“…To collect the biomass from samples, the first 1 L of post-stagnation samples was filtered through 0.2-μm polycarbonate filters (Isopore; Merck Millipore). Regarding pre-stagnation samples, 10 L of samples were concentrated using a hollow fiber ultrafiltration (HFUF) unit with a surface area of 25 m 2 (APS-25SA; Asahi Kasei Medical), following a previously described method ( Liu et al , 2020 ). The recovery ratio of E. coli cells by HFUF was reported as 96.5±8.5% ( Liu et al , 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Regarding pre-stagnation samples, 10 L of samples were concentrated using a hollow fiber ultrafiltration (HFUF) unit with a surface area of 25 m 2 (APS-25SA; Asahi Kasei Medical), following a previously described method ( Liu et al , 2020 ). The recovery ratio of E. coli cells by HFUF was reported as 96.5±8.5% ( Liu et al , 2020 ). Prior to the filtration process using a HFUF unit, a blocking process was conducted by circulating 5% fetal bovine serum (Thermo Fisher Scientific) in the HFUF cartridge for 2‍ ‍min.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of the Microbial Community and Opportunistic Pathogens after Water Stagnation in the Premise Plumbing of a Building

et al. 2022

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In premise plumbing, microbial water quality may deteriorate under certain conditions, such as stagnation. Stagnation results in a loss of disinfectant residual, which may lead to the regrowth of microorganisms, including opportunistic pathogens. In the present study, microbial regrowth was investigated at eight faucets in a building over four seasons in one year. Water samples were obtained before and after 24 h of stagnation. In the first 100‍ ‍mL after stagnation, total cell counts measured by flow cytometry increased 14- to 220-fold with a simultaneous decrease in free chlorine from 0.17–0.36‍ ‍mg L –1 to <0.02‍ ‍mg L –1 . After stagnation, total cell counts were not significantly different among seasons; however, the composition of the microbial community varied seasonally. The relative abundance of Pseudomonas spp. was dominant in winter, whereas Sphingomonas spp. were dominant in most faucets after stagnation in other seasons. Opportunistic pathogens, such as Legionella pneumophila , Mycobacterium avium , Pseudomonas aeruginosa , and Acanthamoeba spp., were below the quantification limit for real-time quantitative PCR in all samples. However, sequences related to other opportunistic pathogens, including L. feeleii , L. maceachernii , L. micdadei , M. paragordonae , M. gordonae , and M. haemophilum , were detected. These results indicate that health risks may increase after stagnation due to the regrowth of opportunistic pathogens.

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“…Another recent study based on the metagenomic approach reported that eDNA retrieved from urban river water that encodes antibiotic resistance determinants included sul1 , tet(A) , ere(A) , bla TEM and qnrD [ 63 ]. eARGs was detected in all the downstream samples suggesting the impact of wastewater discharged into the river may reinforce the enrichment of eARGs [ 63 ]. The authors ascertained that adsorbed eDNA could play an important role in the spread and dissemination of ARGs in surface water.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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Over the past decades, the rising antibiotic resistance bacteria (ARB) are continuing to emerge as a global threat due to potential public health risk. Rapidly evolving antibiotic resistance and its persistence in the environment, have underpinned the need for more studies to identify the possible sources and limit the spread. In this context, not commonly studied and a neglected genetic material called extracellular DNA (eDNA) is gaining increased attention as it can be one of the significant drivers for transmission of extracellular ARGS (eARGs) via horizontal gene transfer (HGT) to competent environmental bacteria and diverse sources of antibiotic-resistance genes (ARGs) in the environment. Consequently, this review highlights the studies that address the environmental occurrence of eDNA and encoding eARGs and its impact on the environmental resistome. In this review, we also brief the recent dedicated technological advancements that are accelerating extraction of eDNA and the efficiency of treatment technologies in reducing eDNA that focuses on environmental antibiotic resistance and potential ecological health risk.

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Consecutive ultrafiltration and silica adsorption for recovery of extracellular antibiotic resistance genes from an urban river

Cited by 34 publications

References 41 publications

High-Throughput Screening of Antimicrobial Resistance Genes and Their Association With Class 1 Integrons in Urban Rivers in Japan

High-Throughput Screening of Antimicrobial Resistance Genes and Their Association With Class 1 Integrons in Urban Rivers in Japan

Dynamics of the Microbial Community and Opportunistic Pathogens after Water Stagnation in the Premise Plumbing of a Building

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

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