Connexin 32 induces pro-tumorigenic features in MCF10A normal breast cells and MDA-MB-231 metastatic breast cancer cells

Adak, Aslı; Unal, Yagmur Ceren; Yucel, Simge; Vural, Zehra; Turan, Fatma Basak; Yalcin-Ozuysal, Özden; Özçivici, Engin; Meşe, Gülistan

doi:10.1016/j.bbamcr.2020.118851

Cited by 13 publications

(10 citation statements)

References 55 publications

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“…On the contrary, Cnx32 overexpression in malignant breast cell line MDA-MB-231 did not alter viability, proliferation rate or migration activity, however, altered the ratio of EMT markers: along with the persistent high expression of vimentin and slug, the expression of E-cadherin decreased. This indicates that Cnx32 overexpression may act as a transformationpromoting event which introduces the element of motility to normal tissue [153]. Another example of the morphogenic field disturbance due to the induction of cell motility was reported for healthy skin and heart tissue subjected to injury under hypoxic conditions.…”

Section: Connexins and Cancer Initiationmentioning

confidence: 91%

“…Thus, overexpression of Cnx32 in normal breast epithelium cell line MCF10A decreased cell viability, turned cell morphology towards mesenchymal phenotype and increased migratory activity of the cells with no changes in their proliferative activity [153]. This signs of epithelial-mesenchymal transition (EMT) were accompanied by simultaneous elevation of protein levels of E-cadherin and vimentin, and of note, Cnx32 overexpression, obtained by lentiviral transfection, directly promoted E-cadherin level elevation.…”

Section: Connexins and Cancer Initiationmentioning

confidence: 92%

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The Multifaceted Role of Connexins in Tumor Microenvironment Initiation and Maintaining

Kutova¹,

Pospelov²,

Balalaeva³

2021

Preprint

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The modern paradigm of studying the processes of carcinogenesis and vital activity of tumor tissues implies increased attention to constituents of tumor microenvironment (TME) and their interactions. These interactions between the cells in TME can be mediated via protein junctions of different types. Connexins (Cnxs) are one of the major contributors to intercellular communication. They form gap junctions responsible for the transfer of ions, metabolites, peptides, miRNA, etc. between neighboring tumor cells as well as between tumor and stromal cells. Cnx hemichannels mediate purinergic signaling and bidirectional molecular transport with the extracellular environment. Additionally, Cnxs were reported to localize in tumor-derived exosomes and facilitate the release of their cargo. A large body of evidence implies that the role of connexins in cancer is multifaceted. Pro- or anti-tumorigenic properties of connexins are determined by their abundance, localization and functionality as well as channel assembly and non-channel functions. In this review we have summarized the data on the Cnxs contribution in TME and to the cancer initiation and progression.

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Section: Connexins and Cancer Initiationmentioning

confidence: 91%

Section: Connexins and Cancer Initiationmentioning

confidence: 92%

The Multifaceted Role of Connexins in Tumor Microenvironment Initiation and Maintaining

Kutova¹,

Pospelov²,

Balalaeva³

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Overexpression of Cx32 proteins in normal and metastatic breast cancer cells led to a more mesenchymal-like phenotype [ 80 ]. Adak and co-workers reported an increased migratory capacity of healthy breast cells, while mesenchymal markers, including vimentin, were further upregulated in the metastatic counterpart, thus presenting, for the first time, the metastasis-stimulating properties of the Cx32 protein in breast cancer [ 80 ]. Heterologous Cx43 protein-composed GJs (Cx43-GJs) have also been linked to the initiation of brain metastatic lesions from both melanoma and breast cancer.…”

Section: Pro-tumorigenic Properties Of Cxs and Gjsmentioning

confidence: 99%

The pro- and anti-tumoral properties of gap junctions in cancer and their role in therapeutic strategies

Oliveira¹,

Verswyvel²,

Smits³

et al. 2022

Redox Biology

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“…Breast cancer (BC) is one of the most frequent malignancy in women, and is associated with a high mortality rate and economic burden [ 1 , 2 ]. Owing to its complicated etiology, poor response and severe side-effects of chemotherapy, both safety and effectiveness are considered as the key challenges to prevent deaths [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Hierarchical drug release designed Au @PDA-PEG-MTX NPs for targeted delivery to breast cancer with combined photothermal-chemotherapy

Cao

et al. 2021

J Nanobiotechnol

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Breast cancer (BC) is the most frequently diagnosed cancer with a low survival rate and one of the major causes of cancer-related death. Methotrexate (MTX) is an anti-tumor drug used in the treatment of BC. Poor dispersion in water and toxic side effects limit its clinical application. Gold nanoparticles (AuNPs), owing to their specific structures and unique biological and physiochemical properties, have emerged as potential vehicles for tumor targeting, bioimaging and cancer therapy. An innovative nano drug-loading system (Au @PDA-PEG-MTX NPs) was prepared for targeted treatment of BC. Au @PDA-PEG-MTX NPs under near infra-red region (NIR) irradiation showed effective photothermal therapy against MDA-MB-231 human BC cells growth in vitro by inducing apoptosis through triggering reactive oxygen species (ROS) overproduction and generating excessive heat. In vivo studies revealed deep penetration ability of Au @PDA-PEG-MTX NPs under NIR irradiation to find application in cancer-targeted fluorescence imaging, and exhibited effective photothermal therapy against BC xenograft growth by inducing apoptosis. Histopathological analysis, cellular uptake, cytotoxicity assay, and apoptosis experiments indicated that Au @PDA-PEG-MTX NPs possessed a good therapeutic effect with high biocompatibility and fewer side effects. This Au NPs drug-loading system achieved specific targeting of MTX to BC cells by surface functionalisation, fluorescence imaging under laser irradiation, combined photothermal-chemotherapy, and pH- and NIR- triggered hierarchical drug release.

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Connexin 32 induces pro-tumorigenic features in MCF10A normal breast cells and MDA-MB-231 metastatic breast cancer cells

Cited by 13 publications

References 55 publications

The Multifaceted Role of Connexins in Tumor Microenvironment Initiation and Maintaining

The Multifaceted Role of Connexins in Tumor Microenvironment Initiation and Maintaining

The pro- and anti-tumoral properties of gap junctions in cancer and their role in therapeutic strategies

Hierarchical drug release designed Au @PDA-PEG-MTX NPs for targeted delivery to breast cancer with combined photothermal-chemotherapy

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