Connexin 30.2 is expressed in mouse pancreatic beta cells

Coronel-Cruz, Cristina; Hernández-Téllez, Beatriz; López-Vancell, R.; López-Vidal, Yolanda; Berumen, Jaime; Castell, Anne-Laure; Pérez-Armendáriz, E. Martha

doi:10.1016/j.bbrc.2013.06.100

Cited by 16 publications

(10 citation statements)

References 30 publications

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“…In the mouse brain, the expression profiles of Cx36 and Cx30.2 are similar, with a high degree of co-expression in striatum, hippocampus and cortex (Kreuzberg et al, 2008 ). Both connexins are also found in β-cells of the pancreas (Coronel-Cruz et al, 2013 ) and chromaffin cells of the adrenal medulla (Kreuzberg et al, 2008 ). Despite the extensive co-distribution of the two connexins, none of the previous studies examined whether they could form heteromeric Cx30.2/Cx36 gap junction channels.…”

Section: Discussionmentioning

confidence: 99%

Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

Meyer

Tetenborg

Greb

et al. 2016

Front. Mol. Neurosci.

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Electrical coupling via gap junctions is an abundant phenomenon in the mammalian retina and occurs in all major cell types. Gap junction channels are assembled from different connexin subunits, and the connexin composition of the channel confers specific properties to the electrical synapse. In the mouse retina, gap junctions were demonstrated between intrinsically photosensitive ganglion cells and displaced amacrine cells but the underlying connexin remained undetermined. In the primary rod pathway, gap junctions play a crucial role, coupling AII amacrine cells among each other and to ON cone bipolar cells. Although it has long been known that connexin36 and connexin45 are necessary for the proper functioning of this most sensitive rod pathway, differences between homocellular AII/AII gap junctions and AII/ON bipolar cell gap junctions suggested the presence of an additional connexin in AII amacrine cells. Here, we used a connexin30.2-lacZ mouse line to study the expression of connexin30.2 in the retina. We show that connexin30.2 is expressed in intrinsically photosensitive ganglion cells and AII amacrine cells. Moreover, we tested whether connexin30.2 and connexin36—both expressed in AII amacrine cells—are able to interact with each other and are deposited in the same gap junctional plaques. Using newly generated anti-connexin30.2 antibodies, we show in HeLa cells that both connexins are indeed able to interact and may form heteromeric channels: both connexins were co-immunoprecipitated from transiently transfected HeLa cells and connexin30.2 gap junction plaques became significantly larger when co-expressed with connexin36. These data suggest that connexin36 is able to form heteromeric gap junctions with another connexin. We hypothesize that co-expression of connexin30.2 and connexin36 may endow AII amacrine cells with the means to differentially regulate its electrical coupling to different synaptic partners.

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Section: Discussionmentioning

confidence: 99%

Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

Meyer

Tetenborg

Greb

et al. 2016

Front. Mol. Neurosci.

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“…Connexin 30.2 (Cx30.2) was found to be highly expressed in mouse β‐cells and vascular endothelial cells and was spatially associated with Cx36. This suggests that these connexin species may form heteromeric connexon hemi‐channels and gap junctions [68]. These findings could explain the heterogeneity of Cx36 gap junction coupling across the islet, as mouse Cx30.2 gap junctions have a lower unitary conductance than Cx36 channels in connexin expressing cell lines [69].…”

Section: Connexin36 Regulation Of Insulin Secretionmentioning

confidence: 97%

New insights into the role of connexins in pancreatic islet function and diabetes

Farnsworth

Benninger

2014

FEBS Letters

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Multi-cellular systems require complex signaling mechanisms for proper tissue function, to mediate signaling between cells in close proximity and at distances. This holds true for the islets of Langerhans, which are multicellular micro-organs located in the pancreas responsible for glycemic control, through secretion of insulin and other hormones. Coupling of electrical and metabolic signaling between islet β-cells is required for proper insulin secretion and effective glycemic control. β-cell specific coupling is established through gap junctions composed of connexin36, which results in coordinated insulin release across the islet. Islet connexins have been implicated in both Type-1 and Type-2 diabetes; however a clear link remains to be determined. The goal of this review is to discuss recent discoveries regarding the role of connexins in regulating insulin secretion, the regulation of connexins within the islet, and recent studies which support a role for connexins in diabetes. Further studies which investigate the regulation of connexins in the islet and their role in diabetes may lead to novel diabetes therapies which regulate islet function and β-cell survival through modulation of gap junction coupling.

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“…Connexins, ephrins, and cadherins, members of the transmembrane family of proteins are expressed in pancreatic islets. The major β-cell connexin is Cx36[171], Cx43, and Cx45 are specifically expressed on intra-islet ECs[172] whereas Cx30.2, recently identified, is expressed at cell-cell junctions in both cell types[173]. …”

Section: Implications Of β-Cell and Endothelial Crosstalk On Islet Trmentioning

confidence: 99%

Intra-islet endothelial cell and β-cell crosstalk: Implication for islet cell transplantation

Narayanan¹,

Loganathan²,

Dhanasekaran³

et al. 2017

WJT

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The intra-islet microvasculature is a critical interface between the blood and islet endocrine cells governing a number of cellular and pathophysiological processes associated with the pancreatic tissue. A growing body of evidence indicates a strong functional and physical interdependency of β-cells with endothelial cells (ECs), the building blocks of islet microvasculature. Intra-islet ECs, actively regulate vascular permeability and appear to play a role in fine-tuning blood glucose sensing and regulation. These cells also tend to behave as “guardians”, controlling the expression and movement of a number of important immune mediators, thereby strongly contributing to the physiology of islets. This review will focus on the molecular signalling and crosstalk between the intra-islet ECs and β-cells and how their relationship can be a potential target for intervention strategies in islet pathology and islet transplantation.

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Connexin 30.2 is expressed in mouse pancreatic beta cells

Cited by 16 publications

References 30 publications

Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

New insights into the role of connexins in pancreatic islet function and diabetes

Intra-islet endothelial cell and β-cell crosstalk: Implication for islet cell transplantation

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