Connective Tissue XII. Stimulating Effects of Estrogens on Collagen Synthesis in Rat Uterine Slices.

Kao, Kung-Ying Tang; Kao, Tang; Hitt, William E.; Bush, Abner T.; McGavack, Thomas H.

doi:10.3181/00379727-117-29504

Cited by 20 publications

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“…The influence of sex hormones on collagen content is also well demonstrated by the fact that the collagen content is approx- imated in both sexes after gonadectomy. It has been said that estrogen accelerates collagen synthesis in the rat uterus (Kao et al 1964) and in rat bone (Orimo et al 1971), whereas it inhibits collagen synthesis in the rat tail tendon (Fischer 1973). It is made clear by the present study that the collagen content of both hip and knee joint capsule is significantly decreased by estrogen or estrogen combined with progesterone.…”

Section: Collagen Contentsupporting

confidence: 66%

Experimental Studies on Connective Tissue of the Capsular Ligament:Influences of Aging and Sex Hormones

Hama

Yamamuro

Takeda

1976

Acta Orthopaedica Scandinavica

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Influences of aging and sex hormones on connective tissue metabolism in the hip joint capsule of Wistar rats were analyzed both biochemically and morphologically. As age advanced, collagen content and collagen fihril diameter of the hip joint capsule tended to increase gradually in both sexes until sexual maturation was reached. Collagen content was significantly greater in males than in females after sexual maturation. The collagen content and fibril diameter were considerably increased by ovariectomy and significantly decreased by the administration of estrogen, o r estrogen combined with progesterone, whereas they were significantly increased by the administration of testosterone in orchiectomized male rats. K e { j uiords: hip joint capsule; aging; sex hormones; colagen content; collagen fibril diameter Accepted 12.vii. 76The most prominent clinical sign of congenital dislocation of the hip in the perinatal period is relaxation of the hip joint capsule. It has been suggested by Andr6n & Borglin (1961 a, b) that female sex hormones participate in the relaxation.The aim of the present experiment is to study the influences of sex hormones on collagen metabolism in the joint capsule by analyzing the collagen content and collagen fibrif diameter. This analysis is an approach to clarifying the causative factors of the relaxation. MATERIALS AND M E T H O D S Treatment of animalsFifty-two Wistar rats, 26 females and 26 males, 3-36 weeks of age, were subjected to an analysis of the influences of aging and 180 Wistar rats, 105 females and 75 males, were subjected to an analysis of the influences of sex hormones.The rats were kept in individual cages and fed on Nihon CLEA Rat Chow and given water ad libitum. They underwent ovariectomy, orchiectomy or sham operation at 3 weeks of age with o r without subsequent hormone treatment.The hormones administered to each animal group were estradiol benzoate (20 pg, per rat), progesterone (10 mg, per rat) and testosterone propionate (2.5 rng, per rat) dissoIved in 0.1 ml of sesame oil. Control groups received sesame oil only. The injections were given twice a week subcutaneously.At 10 weeks of age, the animals were first stunned with a blow and then killed by cervical dislocation. The joint capsules of the hip and knee were dissected free of fatty tissue; the right capsules were prepared for electron microscopic examination and the left ones were hydrolyzed and assayed for hydroxyproline. ACTA ORTHOP. 47, 5 474HIROMICHI HAMA Er AL. Measurement of collagen fibril diameterAfter dissection, the specimens were fixed in 1 per cent osmium tetroxide solution in 0.2 M veronal acetate buffer, pH 7.4, dehydrated through a graded series of ethanol and embedded in Epon 812. Ultrathin sections were cut with glass-knives on an ultramicrotome (Porter-Blum MT-2). The sections were stained with uranyl acetate and examined under the electron microscope (Hitachi HS-7D type) with an accelerated voltage of 50 KV and a direct magnification of 30,000.Four areas in every section were selected at random ...

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Section: Collagen Contentsupporting

confidence: 66%

Experimental Studies on Connective Tissue of the Capsular Ligament:Influences of Aging and Sex Hormones

Hama

Yamamuro

Takeda

1976

Acta Orthopaedica Scandinavica

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“…These sex-hormone receptors are thought to be involved in regulation of the changes in extracellular matrix proteins during decidualization and following parturition (Wewer et al, 1986;Salvador and Tsai, 1973). Indeed, considerable evidence supports the idea that proteins of the ECM are regulated directly or indirectly by ovarian hormones (Salvador and Tsai, 1973;Woessner, 1969;Jeffrey et al, 1975;Komm et al, 1987;Kao et al, 1964Kao et al, , 1969Dyer et al, 1980; …”

Section: Discussionmentioning

confidence: 98%

“…Mandell and Sodek, 1982;Munakata et al, 1984Munakata et al, ,1985Mochizuki and Tojo, 1978;Ozasa et al, 1981;Sakyo et al, 1986). Estrogens increase collagen content in the uterus (Komm et al, 1987;Kao et al, 1964Kao et al, ,1969Dyer et al, 1980), and progesterone regulates uterine collagenase activity and thus uterine collagen content (Gross, 1976;Jeffrey et al, 1975).…”

Section: Des Dosage (Pg)mentioning

confidence: 99%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Brody

Cunha

1989

Am. J. Anat.

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The effects of diethylstilbestrol (DES) treatment on myometrial development from the prenatal to adult period were examined in rats and mice by histologic and immunocytochemical methods using anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells, and by morphometric procedures to assess quantitatively the effect of DES on the expression of cellular orientation in the emerging inner circular myometrial layer. Neonatal rats and mice were treated with DES from day 0 (day of birth) to day 2 with dosages known to perturb myometrial development. Neonatal treatment with DES increased the degree of circular orientation within the uterine mesenchyme, an effect detectable following as little as 24 hr of DES treatment. This effect on spatial organization of the mesenchyme was followed by an increase in the thickness of the actin-positive middle layer (prospective circular myometrium) of uterine mesenchyme during days 3-15; from day 15 onward, however, the circular myometrial layer began to fragment into irregular bundles of smooth muscle, and the longitudinal myometrial layer became thinner and more irregularly organized than controls. Vimentin localization in rats treated with DES neonatally was more intense than in controls within the circularly orientated uterine mesenchyme at 5 days. By 60 days the circular and longitudinal myometrial layers of DES-treated animals showed strands and bundles of vimentin-positive cells, which were not present in controls. Both rats and mice show comparable effects of DES treatment.

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“…It is unclear if metabolism of ECM is regulated hormonally in neonatal rodents because the neonatal ovary is endocrinologically inactive (Csernus, 1986) and because maternal estrogen present at birth is probably bound to alpha-fetoprotein (Henry and Miller, 1986;Raynaud, 1973). In mature uteri, ECM proteins are regulated directly or indirectly by ovarian hormones (Salvador and Tsai, 1973;Woessner, 1969;Jeffrey and Koob, 1973;Jeffrey et al, 1975;Komm et al, 1987;Kao et al, 1964Kao et al, ,1969Dyer et al, 1980;Mandell and Sodek, 1982;Munakata et al, 1984Munakata et al, ,1985Mochizuki and Tojo, 1978;Ozasa et al, 1981;Sakyo et al, 1986). In this regard estrogens have been shown to increase collagen content in the uterus (Komm et al, 1987;Kao et al, 1964Kao et al, , 1969Dyer et al, 1980), and progesterone regulates uterine collagenase activity IGreas, 1976;Jeffrey et al, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…In mature uteri, ECM proteins are regulated directly or indirectly by ovarian hormones (Salvador and Tsai, 1973;Woessner, 1969;Jeffrey and Koob, 1973;Jeffrey et al, 1975;Komm et al, 1987;Kao et al, 1964Kao et al, ,1969Dyer et al, 1980;Mandell and Sodek, 1982;Munakata et al, 1984Munakata et al, ,1985Mochizuki and Tojo, 1978;Ozasa et al, 1981;Sakyo et al, 1986). In this regard estrogens have been shown to increase collagen content in the uterus (Komm et al, 1987;Kao et al, 1964Kao et al, , 1969Dyer et al, 1980), and progesterone regulates uterine collagenase activity IGreas, 1976;Jeffrey et al, 1975). Moreover, during decidualizakon endometrial stromal cells produce an extracellular matrix of laminin, entactin, fibronectin, type IV collagen, and heparan sulfate proteoglycan Faber et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: I. Normal development

Brody¹,

Cunha²

1989

Am. J. Anat.

139

115

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Myometrial development from the prenatal to adult period was examined in rats and mice 1) by histologic and immunocytochemical methods with anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells; and 2) by morphometric procedures to assess quantitatively the expression of cellular orientation in the emerging inner circular myometrial layer. Uterine mesenchymal cells initially were uniformly vimentin-positive, undifferentiated, and randomly oriented during the late fetal period. By the early neonatal period, three mesenchymal layers became recognizable histologically, the middle one of which (prospective circular myometrium) developed distinct circular orientation and differentiated into a layer composed of actin-positive smooth muscle cells. The cells of the inner mesenchymal layer initially exhibited radial orientation. By 10 days postpartum, the outer longitudinal mesenchymal layer differentiated into bundles of smooth muscle cells representing the longitudinal myometrium. The inner mesenchymal layer remained vimentin-positive and differentiated into the randomly ordered endometrial stroma. The cells of the middle and outer mesenchymal layers that were destined to form myometrium initially expressed vimentin throughout and then coexpressed vimentin and actin, but with time vimentin staining disappeared in the maturing smooth muscle cells as they expressed actin.

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Connective Tissue XII. Stimulating Effects of Estrogens on Collagen Synthesis in Rat Uterine Slices.

Cited by 20 publications

References 0 publications

Experimental Studies on Connective Tissue of the Capsular Ligament:Influences of Aging and Sex Hormones

Experimental Studies on Connective Tissue of the Capsular Ligament:Influences of Aging and Sex Hormones

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: I. Normal development

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