Connective tissue of meat. II.—Determination of Hydroxyproline

Abstract

165other of oat and grass hays, corn silage and a grain mixture. Ewes given the former ration produced lambs that developed muscular dystrophy, but the disease did not occur in lambs on the latter ration. The production of dystrophy was associated with a low tocopherolcontent of the ewes' milk, and Whiting et al." thought this was due to the low proportion of a-tocopherol in the clover, lucerne hays and beans. The analyses presented in Table I1 suggest that the so-called deficient diet probably contained more a-tocopherol than the other, since clover and lucerne contain a-tocopherol only, whereas the pand y-compounds form a large proportion of the tocopherols present in oats and maize. The contention of Whiting et al. therefore appears to be incorrect. The results of their experiment, however, emphasize the important interrelationship between vitamin E and other dietary factors.

The effect of development on the composition of a long bone of the pig, rat and fowl

“…Just sufficient HCI was then added to dissolve the precipitated calcium phosphate. After making up to volume with distilled water, samples of the solution were taken for the estimation of Ca, P and N by the methods described by Dickerson & Widdowson (1960b) and for collagen by the Neuman & Logan (1950) procedure with the stronger solution of CuS04 as suggested by Baker, Lampitt & Brown (1953). Rat collagen was assumed to contain 13-4% (Neuman & Logan, 1950) and pig collagen 13-5 % of hydroxyproline (Eastoe, 1955).…”

Factors Affecting the Determination of Hydroxyproline.

“…Sydney, New South Wales, Australia AIDS FOR THE ANALYST Improved Calomel Reference Electrode for Nonaqueous Titration of Halogen Acid Salts of Organic Bases James R. Deily and Leon Donn, Jefferson Chemical Co., Inc., Austin, Texas A procedure whereby halogen acid salts of organic bases could be titrated with perchloric acid in a glacial acetic acid medium has been described by Pifer and Wollish (1). Their procedure involved the addition of mercuric acetate to tie up the halide as the undissociated mercuric halide thus freeing the organic base for titration.…”

Determination of L-hydroxyproline in meat protein by quantitative carbon-13 Fourier transform nuclear magnetic resonance spectrometry

“…Connective tissue imparts strength to tendon, skin, and muscle tissue but increases the toughness of meat as a consequence (2), In order to utilize a wider range of carcass meats, modern meat processing techniques reduce toughness (2) but thereby necessitate a method to detect connective tissue both as a labeling safeguard and as a guide to nutritional value. Collagen, the principal connective protein, is the only protein to contain significant levels of L-hydroxyproline, I, OH H I which is a non-essential amino acid (3). As the two present methods of L-hydroxyproline assay have distinct disadvantages in specificity or sensitivity, 13C Fourier transform nuclear magnetic resonance (FTNMR) spectroscopy was applied to this problem after some initial encouraging results (4).…”