Connections between sister and non-sister telomeres of segregating chromatids maintain euploidy

Warecki, Brandt; Bast, Ian; Tajima, Matthew; Sullivan, William

doi:10.1016/j.cub.2022.11.038

Cited by 5 publications

(8 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the images we obtained correspond to what one expects of tethers, not of ultrafine DNA strands. Connections between separating anaphase chromosomes were described in Drosophila neuroblasts that seem to be different both from ultrafine DNA bridges and from elastic tethers (Warecki et al, 2023). The usual ultrafine DNA bridges do not stain with standard DNA dyes such as DAPI and do not contain histone (Ke et al, 2011;Gemble et al, 2015Gemble et al, , 2020Nielsen et al, 2015;Kong et al, 2023); they rather are identified by staining with associated proteins such as PICH (Nielsen et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The usual ultrafine DNA bridges do not stain with standard DNA dyes such as DAPI and do not contain histone (Ke et al, 2011;Gemble et al, 2015Gemble et al, , 2020Nielsen et al, 2015;Kong et al, 2023); they rather are identified by staining with associated proteins such as PICH (Nielsen et al, 2015). The bridges in Drosophila neuroblasts described by Warecki et al (2023) were identified as containing DNA by staining with DAPI, and some were recognized as containing histones (thought to arise from recruitment of histone to DNA bridges), so they do not seem to be ultrafine DNA strands. Nor do they seem to be the elastic tethers we have described because there are too few per cell.…”

Section: Discussionmentioning

confidence: 99%

“…Drosophila has 2n = 6 autosomes, so one would expect to regularly identify six DAPI-positive bridges in each cell if these structures were tethers and if tethers contained DNA. Warecki et al (2023) saw anaphase "bridges" between chromosomes, primarily at telomeres, but saw them in only 38% of the cells, and mostly in early anaphase. Thus, these are not the same as ultrafine DNA bridges, or the same as the tethers we described, or that were described by the laser experiments; if their results are found to be generally applicable, the bridges would seem to be yet another (third) kind of "tether" or "bridge.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes

Forer,

Otsuka

2023

Life Sci. Alliance

View full text Add to dashboard Cite

Different types of anaphase bridges are reported to form between segregating chromosomes during cell division. Previous studies using laser microsurgery suggested that elastic tethers connect the telomeres of separating anaphase chromosomes in many animal meiotic and mitotic cells. However, structural evidence is lacking for their existence. In this study, by correlating live imaging with electron tomography, we examined whether visible structures connect separating telomeres in meiosis I of crane-fly primary spermatocytes. We found structures extending between separating telomeres in all stages of anaphase. The structures consist of two components: one is darkly stained, looking somewhat like chromatin, whereas the other is more lightly stained, appearing filamentous. Although in early anaphase both structures extend between telomeres, in later anaphase, the darker structure extends shorter distances from the telomeres but the lighter structure still extends between the separating telomeres. From these observations, we deduced that these structures represent the “tethers” inferred from the laser-cutting experiments. Because elastic tethers have been detected in a variety of animal cells, they probably are present during anaphase in all animal cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes

Forer,

Otsuka

2023

Life Sci. Alliance

View full text Add to dashboard Cite

show abstract

“…Regarding the stage of mitosis when mitosis-related DDRs are activated, an interesting question for future study is the role of distinct phases of mitosis, as mitotic cells before or after the metaphase to anaphase transition are massively different in terms of the activity of key proteolytic events that drive mitotic progression. Along these lines, a recent study in Drosophila identified an early anaphase mechanism involving several components such as Polo, the ortholog of PLK1, whereby telomere–telomere connections between segregating sister chromatids are stabilized in neural stem cells with mitotic DNA breaks ( Warecki et al, 2023 ).…”

Section: Mitosis: Dna Damage On a Road Tripmentioning

confidence: 99%

Broken chromosomes heading into mitosis: More than one way to patch a flat tire

Messer,

Fox

2024

Journal of Cell Biology

View full text Add to dashboard Cite

A cell dealing with a broken chromosome in mitosis is like a driver dealing with a flat tire on the highway: damage repair must occur under non-ideal circumstances. Mitotic chromosome breaks encounter problems related to structures called micronuclei. These aberrant nuclei are linked to cell death, mutagenesis, and cancer. In the last few years, a flurry of studies illuminated two mechanisms that prevent mitotic problems related to micronuclei. One mechanism prevents micronuclei from forming during mitosis and involves DNA Polymerase Theta, a DNA repair regulator that patches up broken mitotic chromosomes. A second mechanism is activated after micronuclei form and then rupture, and involves CIP2A and TOPBP1 proteins, which patch micronuclear fragments to promote their subsequent mitotic segregation. Here, we review recent progress in this field of mitotic DNA damage and discuss why multiple mechanisms exist. Future studies in this exciting area will reveal new DNA break responses and inform therapeutic strategies.

show abstract

“…Possibly delays in resolving the terminal recombination events are responsible for the end-to-end linkages and ring configuration late in meiosis. Alternatively, there is evidence for extrachromosomal DNA threads connecting the telomeres of segregating mitotic chromosomes ( Warecki et al. , 2023 ).…”

Section: Chains and Ringsmentioning

confidence: 99%

Remarkable chromosomes and karyotypes: A top 10 list

Sullivan

2024

MBoC

Self Cite

View full text Add to dashboard Cite

Chromosomes and karyotypes are particularly rich in oddities and extremes. Described below are 10 remarkable chromosomes and karyotypes sprinkled throughout the tree of life. These include variants in chromosome number, structure, and dynamics both natural and engineered. This versatility highlights the robustness and tolerance of the mitotic and meiotic machinery to dramatic changes in chromosome and karyotype architecture. These examples also illustrate that the robustness comes at a cost, enabling the evolution of chromosomes that subvert mitosis and meiosis.

show abstract

Connections between sister and non-sister telomeres of segregating chromatids maintain euploidy

Cited by 5 publications

References 79 publications

Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes

Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes

Broken chromosomes heading into mitosis: More than one way to patch a flat tire

Remarkable chromosomes and karyotypes: A top 10 list

Contact Info

Product

Resources

About