Connecting tubules develop from the tip of the ureteric bud in the human kidney

Sarkany, Beatrix; Kovács, Gyula

doi:10.1007/s00418-021-02033-5

Cited by 5 publications

(8 citation statements)

References 30 publications

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“…After the separation of the primitive renal vesicle, a further important step of the installation takes place at the section border between the head and upper conus of the related CD ampulla. Up to date, it is unclear why exactly at this site and by which molecular mechanisms first the adhesion of the distal pole at the primitive renal vesicle takes place, which leads then to an attachment and finally to the lasting link via the future connecting tubule [ 44 – 47 ]. Unknown is also why at the same time and by which kind of molecular linking the proximal pole of the renal vesicle is mounted next to the connecting tubule of an underlying but previously developed nephron.…”

Section: Discussionmentioning

confidence: 99%

Installation of the developing nephron in the fetal human kidney during advanced pregnancy

Minuth

2023

Mol Cell Pediatr

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Background The kidneys of preterm and low birth weight babies reflect vulnerability, since several noxae can evoke the termination of nephron formation. This again leads to oligonephropathy with severe consequences for health in the later life. While the clinical parameters have been intensely investigated, only little is known about the initial traces left by the noxae. For the fetal human kidney, solely the lack of basophilic S-shaped bodies and the reduction in width of the nephrogenic zone were registered. It is not known in how far also the involved progenitor cells, the earlier nephron stages, the collecting duct (CD) ampullae, and the local interstitium are collaterally harmed. Aim The interstitium at the forming nephron is heterogeneously structured. Thereby, it fulfills quite different mastering and integrative tasks. Since data dealing with the installation of a nephron is not available, the microanatomical features were recorded. Results The microscopic specimens show that the installation of the transient stages of nephron anlage is not synchronized. Instead, it is controlled within a nephrogenic compartment of the nephrogenic zone. It starts near the renal capsule by positioning the nephrogenic niche so that the nephrogenic progenitor cells face the epithelial progenitor cell at the tip of a CD ampulla. Then, the induced nephrogenic progenitor cells assimilate in the pretubular aggregate. While its medial part remains opposite the head of the CD ampulla, at its proximal end, the primitive renal vesicle is formed. Only a part of it separates to stick to the section border between the head and conus of the CD ampulla. This marks the link with the future connecting tubule at the distal pole of the extending renal vesicle. Meanwhile, the proximal pole is mounted next to the connecting tubule of an earlier developed nephron. The resulting two-point mounting serves a common elongation of the conus at the CD ampulla and the medial aspect of the comma-shaped body. In the S-shaped body, it supports to defoliate the arising glomerulus and to link it with the perforating radiate artery at its deep lateral aspect. Conclusions The investigation depicts that the installation is an interactive process between the stages of nephron anlage and its structural neighbors. A special meaning has the interjacent interstitium. It is vital for the positioning, shaping, and physiological integration. Due to its special location, this is mainly exposed to noxae.

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Section: Discussionmentioning

confidence: 99%

Installation of the developing nephron in the fetal human kidney during advanced pregnancy

Minuth

2023

Mol Cell Pediatr

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“…While the primitive renal vesicle develops, the interface is interrupted by its adhesion at the section border between the head and conus of the CD ampulla. Recent findings from different groups have shown that this serves the primary linking with the future connecting tubule [45][46][47]. During the ongoing development, the mature, extending, and extended renal vesicles (Figs.…”

Section: Narrow Interstitial Cleft At the Medial Aspect Of The Develo...mentioning

confidence: 98%

The interstitium at the developing nephron in the fetal kidney during advanced pregnancy — a microanatomical inventory

Minuth

2022

Mol Cell Pediatr

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Background A series of noxae can evoke the termination of nephron formation in preterm and low birth weight babies. This results in oligonephropathy with severe consequences for health in the later life. Although the clinical parameters have been extensively investigated, little is known about the initial damage. Previous pathological findings indicate the reduction in width of the nephrogenic zone and the lack of S-shaped bodies. Current morphological investigations suggest that due to the mutual patterning beside the forming nephron, also its structural neighbors, particularly the interjacent interstitium, must be affected. However, beside the findings on integrative and mastering functions, systematic microanatomical data explaining the configuration of the interstitium at the developing nephron in the fetal kidney during advanced pregnancy is not available. Therefore, this work explains the typical features. Results The generated data depicts that the progenitor cells, nephrogenic niche, pretubular aggregate, and mesenchymal-to-epithelial transition are restricted to the subcapsular interstitium. During the proceeding development, only the distal pole of the renal vesicles and comma- and S-shaped bodies stays in further contact with it. The respective proximal pole is positioned opposite the peritubular interstitium at the connecting tubule of an underlying but previously formed nephron. The related medial aspect faces the narrow peritubular interstitium of a collecting duct (CD) ampulla first only at its tip, then at its head, conus, and neck, and finally at the differentiating CD tubule. The lateral aspect starts at the subcapsular interstitium, but then it is positioned along the wide perivascular interstitium of the neighboring ascending perforating radiate artery. When the nephron matures, the interstitial configuration changes again. Conclusions The present investigation illustrates that the interstitium at the forming nephron in the fetal kidney consists of existing, transient, stage-specific, and differently far matured compartments. According to the developmental needs, it changes its shape by formation, degradation, fusion, and rebuilding.

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“…Tissue sections were counterstained with Mayer's hematoxylin (Lillie's modification, DAKO), and after 10 s of bluing in ammoniumhydroxide solution, they were mounted by Glycergel (DAKO). Each antibody was validated for specificity by immunohistochemistry of normal adult kidneys [12]. For negative control, the primary antibody was omitted.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…First, we searched for UB-derived tubular structures by RET, ROBO1, and SLIT2 immunohistochemistry in NR and WT. Subsequently, we have analyzed the expression of CA2 marking progenitors of intercalated (IC) and principal (PC) cells, ATP6V1B1, ATP6V0D2, SLC4A1, and SLC26A4, which are specific for immature and mature aand b-IC cells and AQP2 specific for PCs [12].…”

Section: Introductionmentioning

confidence: 99%

Novel concept of Wilms’ tumor development: involvement of pluripotential cells of ureteric bud

Sarkany,

Kuthi,

Kovacs

2023

Human Pathology

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Connecting tubules develop from the tip of the ureteric bud in the human kidney

Cited by 5 publications

References 30 publications

Installation of the developing nephron in the fetal human kidney during advanced pregnancy

Installation of the developing nephron in the fetal human kidney during advanced pregnancy

The interstitium at the developing nephron in the fetal kidney during advanced pregnancy — a microanatomical inventory

Novel concept of Wilms’ tumor development: involvement of pluripotential cells of ureteric bud

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