Conjunctival swabs and corneoscleral rim cultures from corneal transplantation donors as possible early indicators for posttransplant endopthalmitis

Abstract

It is recommended that the corneoscleral buttons be removed as soon as possible. Cultures of conjunctival swabs collected from donors after disinfection may be useful in determining treatment for postoperative infections occurring after corneal transplantation.

Analysis of Intraocular Lens Biofilms and Fluids After Long-Term Uncomplicated Cataract Surgery

“…This approach also guaranteed our further molecular analyses of intraocular fluids by PCR. As previously reported (21), even though globes were kept in an antimicrobial maintenance solution, a high incidence of contamination on the surface of donor eyes was observed prior to decontamination. The microbial flora on the surface of ocular globes before decontamination was similar to that found on the conjunctivae and eyelids of healthy eyes (28).…”

“…Initial treatment with the antimicrobial maintenance solution eliminated the external microbiota in half of the globes (n = 10) selected for decontamination control efficacy. The microbial flora found on the ocular surface before treatment was similar to the microbial flora found in the conjunctiva and eyelids of healthy eyes (21). More than one species was isolated in most studied globes; commensal species predominated, with seven isolates of coagulase-negative staphylococci (33.3%), six isolates of Corynebacterium species (28.6%) and two isolates of Micrococcus spp.…”

Conjunctival and intraocular swabs for the microbiological assessment of donor corneas

“…; Price & Price ; Matsumoto et al. ). Consequently, we found a high positive rate of conjunctival swabs, when incubated at 22.5°C (22.8%), and even higher, when incubated at 32.5°C (32.5%), which agrees with the literature: Matsumoto et al.…”

“…Matsumoto et al. () could show an effect of death‐to‐harvest time on positivity rates of conjunctival swabs. Microorganisms were detected significantly more frequently when death‐to‐harvest time was larger than 10 hr, which suggests microbial growth after the death of the donor.…”

“…proved a contamination rate of cadaver conjunctiva of 36.7% after disinfection with gentamicin and incubation at 35°C (Matsumoto et al. ). Li et al.…”