Conjunctival Goblet Cell Densities in Ocular Surface Disease

Objective To investigate the effects of chronic smoking on ocular surface and tear functions. Methods Fifteen right eyes of 15 healthy chronic smokers (9 men, 6 women; age range: 36-47 years) who smoked 20 cigarettes per day for 20 years and 20 eyes of 20 control nonsmokers (12 men, 8 women; age range: 38-43 years) were included in this prospective study. All subjects underwent measurements of breath and haemoglobin CO concentration, tear lipid layer interferometry, evaporimetry, tear film break-up time (TBUT), Schirmer's I test, corneal fluorescein staining, conjunctival impression, and brush cytology. Results The mean Hb CO level was significantly higher in smokers compared to non-smokers. TBUT was also significantly shorter in smokers. Tear lipid layer showed significant slowing in spread over the tear film with a concomitant significant increase in tear evaporation rate. Conjunctival impression cytology revealed significant loss of goblet cells and squamous metaplasia in smokers. Brush cytology showed significant conjunctival neutrophil infiltration in smoker subjects. Conclusion Chronic smoking induced distinctive quantitative and qualitative disturbances on the ocular surface health.

show abstract

“…The specimens were also assigned a grade of conjunctival epithelial squamous metaplasia according to Nelson's grading scheme. 37 …”

Section: Impression Cytology Parameterssupporting

confidence: 44%

Alterations of the tear film and ocular surface health in chronic smokers

Matsumoto

Doğru

Goto

et al. 2008

Eye

View full text Add to dashboard Cite

show abstract

“…8,9 Mucins are typically assessed on the basis of TFBUT, biochemical analysis of tears, and cytologic or histologic evaluation of goblet cell density. 8,[10][11][12] Tear assays are constantly evolving, and a diversity of new techniques has recently been described in people, including wavefront aberrometry, corneal topography, advanced imaging (optical coherence tomography and confocal microscopy), and visual acuity testing. 13 Use of these techniques has been increasingly described for people and, to a lesser extent, dogs.…”

mentioning

confidence: 44%

Reference values, intertest correlations, and test-retest repeatability of selected tear film tests in healthy cats

Sebbag

Kass

Maggs

2015

javma

View full text Add to dashboard Cite

Objective—To determine reference values, intertest correlations, and test-retest repeatability of Schirmer tear test 1 (STT-1), phenol red thread test (PRTT), tear film breakup time (TFBUT), tear osmolarity, and meibometry in healthy cats. Design—Evaluation study. Animals—135 healthy domestic cats aged 0.5 to 12.8 years. Procedures—Each test was performed once in 120 cats and repeated in 40. Pearson correlation was used to assess correlation among tests. Intraclass correlation coefficients (ICCs) and 95% limits of agreement (LOA) were used to evaluate test-retest repeatability. Results—Median (95% central range) values were 18 mm/min (9 to 34 mm/min) for STT-1, 29 mm/15 s (15 to 37 mm/15 s) for PRTT, 12.4 seconds (9.1 to 17.7 seconds) for TFBUT, 322 mOsm/L (297 to 364 mOsm/L) for osmolarity, and 32 meibometry units (MU; 11 to 114 MU) for peak meibometry value. The STT-1 and PRTT values were positively correlated. Age was weakly associated with TFBUT and osmolarity. Meibometry measurements were higher for strips that contacted the tear film (285 MU) than for those that touched the eyelid margin only (32 MU). All ICCs were < 0.75, and 95% LOA were wide. Conclusions and Clinical Relevance—Tear deficiency should be suspected in cats with STT-1 < 9 mm/min, PRTT < 15 mm/15 s, or TFBUT < 9 to 10 seconds. Generally poor correlation among tests suggested that thorough tear film analysis requires performance of multiple tests in concert. Relatively poor test-retest repeatability should be considered when repeated tests are used to monitor tear film dysfunction and response to treatment.

show abstract

“…Nelson JD et al have counted the goblet cell but counting of goblet cells were not done in this study. 8,9 12 cases have refractory error and 2 cases of watering eyes had a normal cytology. 18 cases of conjunctivitis had inflammation on cytology.…”

Section: Resultsmentioning

confidence: 43%

Role of conjunctival impression cytology in various diseases of eye

et al. 2016

View full text Add to dashboard Cite

INTRODUCTIONConjunctival impression cytology is a recently introduced method for study and diagnosis of eye diseases affecting conjunctiva. A cellulose acetate filter paper is applied on the conjunctival surface to remove the superficial layer of the conjunctiva. The sample is then stained by PAS and Haematoxylin and cytological analysis is done.This method was first used by Egbert PR et al to study conjunctival goblet cells.1 This method can be used to detect dry eye syndrome, vitamin A deficiency and trachoma, metaplastic and dysplastic changes can also be diagnosed.2 Early diagnosis of neoplasm of eye can be done by this method. This technique can also be applied for documenting sequential changes in conjunctiva over the time, monitoring effect of treatment and staging conjunctival squamous metaplasia, as investigational tool for analyzing ocular surface disease with immunostaining and DNA analysis. Aim of our study is to observe the cellular changes in conjunctival superficial layer in various diseases of eye and establishing the diagnosis and helping in management of eye diseases. METHODSCellulose acetate filter paper is used to collect specimen. Cellulose acetate paper is cut in size of 3 x 10mm with diagonal end. Eye is anesthetized by one drop of paracain. Palpabral fissure is widened by retracting eye lid with finger and thumb of one hand, with other hand filter paper applied over the bulbar conjunctiva and left for 4-6 seconds. 4 Then filter paper is removed with forceps in a peeling motion.The specimen is then fixed by dipping in a fixative containing glacial acetic acid, formaldehyde and ethyl alcohol in ratio 1:1:20 for 10 minutes.5 Then it is rinsed in tap water for two minutes. The strip is dipped in 0.05% periodic Schiff reagent for 8 minutes sodium metabisulfite for 2 minutes and in Haematoxylin for 30 seconds. The strip is rinsed for 2 minutes with tap water after each step above. Now 95% ethyl alcohol is applied over strip for 2 minutes. The strip is dehydrated with ABSTRACT Background: Conjunctival impression cytology is a recent method to diagnose various eye diseases. Aim of this study is to observe the cellular changes in conjunctival superficial layer in various diseases of eye and establishing the diagnosis and helping in the management of the disease Methods: Cellulose acetate filter paper is used to take impression from the bulbar conjunctiva. Results: 80 cases were studied for a span of 3 years. Conclusions: Conjunctival impression cytology is a useful method of screening, diagnosing and managing eye disease.

show abstract

Conjunctival Goblet Cell Densities in Ocular Surface Disease

Cited by 290 publications

References 8 publications

Alterations of the tear film and ocular surface health in chronic smokers

Alterations of the tear film and ocular surface health in chronic smokers

Reference values, intertest correlations, and test-retest repeatability of selected tear film tests in healthy cats

Role of conjunctival impression cytology in various diseases of eye

Contact Info

Product

Resources

About