Conjunctival dysbiosis in mucosa-associated lymphoid tissue lymphoma

Asao, Kazunobu; Hashida, Noriyasu; Ando, Satoru; Motooka, Daisuke; Kurakami, Hiroyuki; Nakamura, Shota; Yamashita, Daisuke; Maruyama, Kouichi; Kawasaki, Satoshi; Yamada, Tomomi; Iida, Tetsuya; Nishida, Kohji

doi:10.1038/s41598-019-44861-5

Cited by 20 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1,[21][22][23] Since 2001, and especially over the past decade, a number of articles have been published regarding the analysis of the conjunctival microbiome via the use of 16S rRNA gene sequencing. 14,15,[24][25][26][27][28][29][30][31] However, and to the best of our knowledge, no previous studies have reported analyzing the detailed microbiome of meibum via 16S rRNA gene sequencing, 32,33 so it has yet to be elucidated. Although one published study did investigate the meibomian gland microbiome via 16S rRNA gene sequencing for the strain once isolated from the culture of the meibum, some of the dominant anaerobic commensal bacteria, such as P. acnes, were not detected in that meibum.…”

mentioning

confidence: 99%

The Microbiome of the Meibum and Ocular Surface in Healthy Subjects

Suzuki

Sutani

Nakai

et al. 2020

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

The purpose of this study was to investigate the microbiome in the meibum, conjunctival sac, and eyelid skin in young and elderly healthy subjects, and analyze the effect that age, sex, and region have on microbiome composition. METHODS. This study involved 36 healthy subjects (young-age subjects: 9 men/9 women, age range: 20-35 years; elderly age subjects: 9 men/9 women, age range: 60-70 years). In all subjects, lower-eyelid meibum, lower conjunctival sac, and lower-eyelid skin specimens were collected from one eye, and then stored at-20°C. Taxonomic composition of the microbiome was obtained via 16S rRNA gene sequencing, and then analyzed. RESULTS. The meibum microbiome showed a high α-diversity (within-community diversity), particularly in the young subjects. However, in approximately 30% of the elderly subjects, a low-diversity microbiome dominated by Corynebacterium sp. or Neisseriaceae was observed. In the young subjects, the microbiome of the meibum resembled that of the conjunctival-sac, yet in the elderly subjects, the microbiome of the conjunctival-sac became more similar to that of the eyelid skin. The eyelid-skin microbiome was relatively simple, and was typically dominated by Propionibacterium acnes in the young subjects, or by Corynebacterium sp. or Neisseriaceae in the elderly subjects. In both age groups, no significant difference was seen between the men and women in regard to the meibum, conjunctival-sac, and eyelid-skin microbiome. CONCLUSIONS. Our findings confirmed that the meibum of healthy adult-age subjects harbors highly diverse microbiota, and revealed that the meibum microbiome, especially the decrease of its diversity, alters with aging and may affect the homeostasis of the ocular surface.

show abstract

mentioning

confidence: 99%

The Microbiome of the Meibum and Ocular Surface in Healthy Subjects

Suzuki

Sutani

Nakai

et al. 2020

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…This study found no significant difference in diversity between the TAO and control groups, which was similar to allergic rhinoconjunctivitis ( Yau et al., 2019 ), Sjögren’s syndrome ( de Paiva et al., 2016 ), and conjunctival mucosa-associated lymphoid tissue lymphoma ( Asao et al., 2019 ). The reason for this result may be related to the small sample size of the control group in this study, which reduced the statistical power.…”

Section: Discussionmentioning

confidence: 63%

Comparison of the ocular surface microbiota between thyroid-associated ophthalmopathy patients and healthy subjects

Dong

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

PurposeThyroid-associated ophthalmopathy (TAO) is a chronic autoimmune disease. In this study, high-throughput sequencing was used to investigate the diversity and composition of the ocular microbiota in patients with TAO.MethodsPatients with TAO did not receive treatment for the disease and did not have exposed keratitis. Patients with TAO (TAO group) and healthy individuals (control group) were compared. All samples were swabbed at the conjunctival vault of the lower eyelid. The V3 to V4 region of the 16S rDNA was amplified using polymerase chain reaction and sequenced on the Illumina HiSeq 2500 Sequencing Platform. Statistical analysis was performed to analyze the differences between the groups and the correlation between ocular surface microbiota and the disease. The ocular surface microbiota of patients and healthy individuals were cultured.ResultsThe ocular surface microbiota structure of TAO patients changed significantly. The average relative abundance of Bacillus and Brevundimonas increased significantly in the TAO group. Corynebacterium had a significantly decreased relative abundance (P<0.05). Paracoccus, Haemophilus, Lactobacillus, and Bifidobacterium were positively correlated with the severity of clinical manifestations or disease activity (P<0.05). Bacillus cereus and other opportunistic pathogens were obtained by culture from TAO patients.ConclusionsThis study found that the composition of ocular microbiota in patients with TAO was significantly different from that in healthy individuals. The ocular surface opportunistic pathogens, such as Bacillus, Brevundimonas, Paracoccus, and Haemophilus in TAO patients, increase the potential risk of ocular surface infection. The findings of this study provide a new avenue of research into the mechanism of ocular surface in TAO patients.

show abstract

“…Of note, two anophthalmic sockets had detectable Clostridium sequences while none was seen in contralateral control eyes. Clostridium sequences have been detected occasionally in previous studies as a component of normal conjunctiva 3 , 19 .…”

Section: Resultsmentioning

confidence: 83%

Elevated levels of Merkel cell polyoma virus in the anophthalmic conjunctiva

Siegal

Gutowski

Akileswaran

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The human ocular surface hosts a paucibacterial resident microbiome and virome. The factors contributing to homeostasis of this mucosal community are presently unknown. To determine the impact of ocular enucleation and prosthesis placement on the ocular surface microbiome, we sampled conjunctival swabs from 20 anophthalmic and 20 fellow-eye intact conjunctiva. DNA was extracted and subjected to quantitative 16S rDNA PCR, biome representational karyotyping (BRiSK), and quantitative PCR (qPCR) confirmation of specific organisms. 16S ribosomal qPCR revealed equivalent bacterial loads between conditions. Biome representational in silico karyotyping (BRiSK) demonstrated comparable bacterial fauna between anophthalmic and intact conjunctiva. Both torque teno virus and Merkel cell polyoma virus (MCPyV) were detected frequently in healthy and anophthalmic conjunctiva. By qPCR, MCPyV was detected in 19/20 anophthalmic samples compared with 5/20 fellow eyes. MCPyV copy number averaged 891 copies/ng in anophthalmic conjunctiva compared with 193 copies/ng in fellow eyes (p < 0.001). These results suggest that enucleation and prosthesis placement affect the ocular surface flora, particularly for the resident virome. As MCPyV has been shown to be the etiologic cause of Merkel cell carcinoma, understanding the mechanisms by which the ocular surface regulates this virus may have clinical importance.

show abstract

Conjunctival dysbiosis in mucosa-associated lymphoid tissue lymphoma

Cited by 20 publications

References 42 publications

The Microbiome of the Meibum and Ocular Surface in Healthy Subjects

The Microbiome of the Meibum and Ocular Surface in Healthy Subjects

Comparison of the ocular surface microbiota between thyroid-associated ophthalmopathy patients and healthy subjects

Elevated levels of Merkel cell polyoma virus in the anophthalmic conjunctiva

Contact Info

Product

Resources

About