2015
DOI: 10.4238/2015.october.29.2
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Conjunctiva reconstruction by induced differentiation of human amniotic epithelial cells

Abstract: ABSTRACT. In this study, we aimed to investigate the feasibility of directed differentiation of human amniotic epithelial cells into conjunctival epithelium under specific conditions as well as of constructing tissueengineered conjunctiva for ocular surface reconstruction. Human amniotic epithelial cells were cultured with induced denuded conjunctival matrix and conjunctival homogenate. Immunohistochemistry of cytokeratin-4, cytokeratin-13, and muc5ac as well as PAS staining were performed. The concentration o… Show more

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Cited by 17 publications
(11 citation statements)
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“…These methods may be suitable for the reconstruction of the corneal epithelium. Additionally, hAECs were able to differentiate into conjunctival epithelium-like cells with partial physiological function upon culture with induced-denuded conjunctival matrix and conjunctival homogenate [ 49 ].…”
Section: Methodsmentioning
confidence: 99%
“…These methods may be suitable for the reconstruction of the corneal epithelium. Additionally, hAECs were able to differentiate into conjunctival epithelium-like cells with partial physiological function upon culture with induced-denuded conjunctival matrix and conjunctival homogenate [ 49 ].…”
Section: Methodsmentioning
confidence: 99%
“…Conjunctival goblet cells secrete mucins that are glycoproteins that play an important role in the ocular surface barrier function [43]. Numerous studies have revealed that ECM from decellularized tissues may provide a vital and functional microenvironment to support the growth and differentiation of goblet cells [44]. Comparable goblet cells embedded in the epithelium were observed on top of DPP-Asp-hEGF, revealing no significant difference in the average count compared with NRC at day 28 (NRC: 6:80 ± 2:17, 9 Stem Cells International DPP-Asp-hEGF: 6:20 ± 1:92, counts/per vision, p > 0:05).…”
Section: Discussionmentioning
confidence: 99%
“…An early attempt was published by Yang et al in 2015, in which the feasibility of the directed differentiation of human amniotic epithelial cells into the conjunctival epithelium was tested [ 80 ]. The transformation of conjunctival epithelial cells after AM transplantation to repair conjunctival damage due to burns had previously been reported [ 81 , 82 ].…”
Section: Potential Of Advanced Therapy Medicinal Products (Atmps) To Improve Conjunctival Treatmentmentioning
confidence: 99%