Conjugative transfer of the transposon Tn919 to lactic acid bacteria

Hill, Colin

doi:10.1016/0378-1097(85)90376-3

Cited by 13 publications

(23 citation statements)

References 6 publications

(19 reference statements)

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“…Another strategy which has considerable potential is the use of transposonal mutagenesis with the related tetracycline-resistant transposons Tn919 and Tn916 [82], which may be precisely excised from E. coli with high efficiency [831. Both transposons have been transferred from S. faecalis into lactic streptococci by various conjugation methods [48,84,85] and appeared to integrate at random in several strains [48,85]. In this way genetically labelled mutants have been obtained by insertional inactivation, as has been reported for Tn916 mutation of the S. lactis gene(s) encoding malolactic fermentation [86].…”

Section: Gene Cloning Strategiesmentioning

confidence: 99%

Gene cloning and expression in lactic streptococci

Vos¹

1987

FEMS Microbiology Letters

266

157

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SUMMARYRecent developments have made the mesophilic lactic streptococci, widely used in dairy fermentations, accessible to genetic manipulation. Several host-vector systems have been described which currently are used in the cloning and expression of homologous and heterologous genes. The essential elements of these systems, the various cloning strategies and the first successful cloning experiments are described with emphasis on the molecular organization of proteinase genes. In addition, the organization and nucleotide sequence of signals which are involved in gene expression in lactic streptococci are summarized. 0168-6445/87/$03.50 © 1987 Federation of European Microbiological Societies PF = plasmid-free; ND-not described; b NCDO strain; lac-= lactose-deficient; rec-= recombination-deficient.

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Section: Gene Cloning Strategiesmentioning

confidence: 99%

Gene cloning and expression in lactic streptococci

Vos¹

1987

FEMS Microbiology Letters

266

157

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“…Heterologous transposons introduced into Lc. laetis include Tn916 (originally isolated from E. faecalis D16) and Tn919 from Streptococcus sanguis (Hill et al, 1985). Insertial inactivation of genes for maltose metabolism, citrate utilization and malolactic 196 THE GENERA OF LACTIC ACID BACTERIA Gasson and Fitzgerald (1994).…”

Section: ) Protoplast Fusion -Of Historical Interest Only (5) Transfomentioning

confidence: 99%

The Genera of Lactic Acid Bacteria

Wood¹,

Holzapfel²

1995

186

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“…sanguis FC1 [78] respectively. They have been introduced into a variety of L lactis strains [79][80][81][82] and Tng19 has also been transferred to Lactobacillus plantarum and Leuconostoc rnesenteroides [79]. They have been introduced into a variety of L lactis strains [79][80][81][82] and Tng19 has also been transferred to Lactobacillus plantarum and Leuconostoc rnesenteroides [79].…”

Section: Use Of Heterologous Transposonsmentioning

confidence: 99%

In vivo genetic systems in lactic acid bacteria

Gasson

1990

FEMS Microbiology Letters

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A review of in vivo genetic systems covers the key features of transduction and conjugation but emphasises the intramolecular and intermolecular DNA interactions that are often associated with these processes. As well as the transfer of many lactose plasmids, conjugal transfer of nisin genes and the use of conjugation to construct bacteriophage‐resistant dairy starter cultures are discussed. The discovery and characterization of insertion sequences in Lactobacillus and Lactococcus and the exploitation of heterologous conjugation and transposition systems in the lactic acid bacteria are described.

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Conjugative transfer of the transposon Tn919 to lactic acid bacteria

Cited by 13 publications

References 6 publications

Gene cloning and expression in lactic streptococci

Gene cloning and expression in lactic streptococci

The Genera of Lactic Acid Bacteria

In vivo genetic systems in lactic acid bacteria

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