Conjugative transfer of tet(S) between strains of Enterococcus faecalis is associated with the exchange of large fragments of chromosomal DIMA

Francois, Bénédicte; Charles, Murielle; Courvalin, Patrice

doi:10.1099/00221287-143-7-2145

Cited by 30 publications

(12 citation statements)

References 31 publications

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“…The identification of conjugatively mobile tetM determinants in these organisms is a cause for concern, as resistance might transfer both to and from other gut bacteria, including enterococci and streptococci [21]. The transfer of tetracycline resistance between L. monocytogenes and E. faecalis confirms that enterococci might act as a reservoir of genes for L. monocytogenes, a finding consistent with other recent data on inducible transfer of resistance in the gastrointestinal tract [13,[22][23][24].…”

Section: à7supporting

confidence: 84%

Transferable tetracycline resistance in Listeria monocytogenes from food in Italy

Pourshaban¹,

Ferrini²,

Mannoni³

et al. 2002

Journal of Medical Microbiology

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Mechanisms of tetracycline resistance were investigated in two recent Listeria monocytogenes isolates from food, with L. innocua 52P tet r as a control. Tetracycline resistance was transferred conjugatively from all three strains to L. ivanovii and from one isolate and the control to Enterococcus faecalis. Molecular analysis demonstrated a chromosomal location for the tet determinant, which was identified as tetM in all cases. These studies are the first to show that L. monocytogenes from food could be a source of tetracycline resistance genes able to spread to other micro-organisms.

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Section: à7supporting

confidence: 84%

Transferable tetracycline resistance in Listeria monocytogenes from food in Italy

Pourshaban¹,

Ferrini²,

Mannoni³

et al. 2002

Journal of Medical Microbiology

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“…Tet(S) was first detected in Listeria monocytogenes BM4210, where it is carried by self-transferable plasmids (6). It has also been found in Enterococcus faecalis, where it is integrated into the chromosomal DNA (7,10), and in Lactococcus spp., where it is located on a conjugative plasmid (27).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Tetracycline Resistance Genes in Oral Bacteria

Villedieu

Diaz-Torres

Hunt

et al. 2003

Antimicrob Agents Chemother

120

105

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Tetracycline is a broad-spectrum antibiotic used in humans, animals, and aquaculture; therefore, many bacteria from different ecosystems are exposed to this antibiotic. In order to determine the genetic basis for resistance to tetracycline in bacteria from the oral cavity, saliva and dental plaque samples were obtained from 20 healthy adults who had not taken antibiotics during the previous 3 months. The samples were screened for the presence of bacteria resistant to tetracycline, and the tetracycline resistance genes in these isolates were identified by multiplex PCR and DNA sequencing. Tetracycline-resistant bacteria constituted an average of 11% of the total cultivable oral microflora. A representative 105 tetracycline-resistant isolates from the 20 samples were investigated; most of the isolates carried tetracycline resistance genes encoding a ribosomal protection protein. The most common tet gene identified was tet(M), which was found in 79% of all the isolates. The second most common gene identified was tet(W), which was found in 21% of all the isolates, followed by tet(O) and tet(Q) (10.5 and 9.5% of the isolates, respectively) and then tet(S) (2.8% of the isolates). Tetracycline resistance genes encoding an efflux protein were detected in 4.8% of all the tetracycline-resistant isolates; 2.8% of the isolates had tet(L) and 1% carried tet(A) and tet(K) each. The results have shown that a variety of tetracycline resistance genes are present in the oral microflora of healthy adults. This is the first report of tet(W) in oral bacteria and the first report to show that tet(O), tet(Q), tet(A), and tet(S) can be found in some oral species.

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“…Plasmid-associated transfer of chromosomal DNA between E. faecalis strains was inferred in 1978 by Franke and colleagues (27), who observed transfer of selectable markers in the presence of coresident transmissible plasmids, but not from a plasmid-free donor. Francois et al (28) observed transfer of tetS from E. faecalis BM4210 to JH2-2 and OG1RF only in the presence of conjugative plasmid, pIP825, and acquisition of the 40-kb tetS element was associated with movement of large pieces of the chromosome. ICE have also been implicated in transfer of chromosomal DNA in E. faecalis (29).…”

Section: Discussionmentioning

confidence: 99%

Mechanism of chromosomal transfer of Enterococcus faecalis pathogenicity island, capsule, antimicrobial resistance, and other traits

Manson

Hancock²,

Gilmore³

2010

Proc. Natl. Acad. Sci. U.S.A.

178

144

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The Enterococcus faecalis pathogenicity island (PAI) encodes known virulence traits and >100 additional genes with unknown roles in enterococcal biology. Phage-related integration and excision genes, and direct repeats flanking the island, suggest it moves as an integrative conjugative element (ICE). However, transfer was observed not to require these genes. Transfer only occurred from donors possessing a pheromone responsive-type of conjugative plasmid, and was invariably accompanied by transfer of flanking donor chromosome sequences. Deletion of plasmid transfer functions, including the cis -acting origin of transfer ( oriT ), abolished movement. In addition to demonstrating PAI movement by a mechanism involving plasmid integration, we observed transfer of a selectable marker placed virtually anywhere on the chromosome. Transfer of this selectable marker was observed to be accompanied by chromosome-chromosome transfer of vancomycin resistance, MLST markers, and capsule genes as well. Plasmid mobilization therefore appears to be a major mechanism for horizontal gene transfer in the evolution of antibiotic resistant E. faecalis strains capable of causing human infection.

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Conjugative transfer of tet(S) between strains of Enterococcus faecalis is associated with the exchange of large fragments of chromosomal DIMA

Cited by 30 publications

References 31 publications

Transferable tetracycline resistance in Listeria monocytogenes from food in Italy

Transferable tetracycline resistance in Listeria monocytogenes from food in Italy

Prevalence of Tetracycline Resistance Genes in Oral Bacteria

Mechanism of chromosomal transfer of Enterococcus faecalis pathogenicity island, capsule, antimicrobial resistance, and other traits

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