Conjugative transfer and autonomous replication of a promiscuous IncQ plasmid in the cyanobacterium Synechocystis PCC 6803

Kreps, Sabine; Ferino, F.; Mosrin, Christine; Gerits, J; Mergeay, Max; Thuriaux, Pierre

doi:10.1007/bf00280378

Cited by 50 publications

(22 citation statements)

References 31 publications

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“…By using helper plasmids, the IncQ plasmids have been successfully mobilized to a large number of hosts including a wide range of gram-negative bacteria, several gram-positive bacteria including Arthrobacter spp. (52), Streptomyces lividans, and Mycobacterium smegmatis (29), cyanobacteria such as Synechococcus (43), and plant (13) and animal (99) cells. Likewise pTF-FC2 has been mobilized from E. coli to Pseudomonas fluorescens (37) and from Agrobacterium tumefaciens to plant cells (Rawlings, unpublished).…”

Section: Mobilization Regionsmentioning

confidence: 99%

Comparative Biology of IncQ and IncQ-Like Plasmids

Rawlings

Tietze

2001

Microbiol Mol Biol Rev

136

124

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Plasmids belonging to Escherichia coli incompatibility group Q are relatively small (approximately 5 to 15 kb) and able to replicate in a remarkably broad range of bacterial hosts. These include gram-positive bacteria such as Brevibacterium and Mycobacterium and gram-negative bacteria such as Agrobacterium, Desulfovibrio, and cyanobacteria. These plasmids are mobilized by several self-transmissible plasmids into an even more diverse range of organisms including yeasts, plants, and animal cells. IncQ plasmids are thus highly promiscuous. Recently, several IncQ-like plasmids have been isolated from bacteria found in environments as diverse as piggery manure and highly acidic commercial mineral biooxidation plants. These IncQ-like plasmids belong to different incompatibility groups but have similar broad-host-range replicons and mobilization properties to the IncQ plasmids. This review covers the ecology, classification, and evolution of IncQ and IncQ-like plasmids

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Section: Mobilization Regionsmentioning

confidence: 99%

Comparative Biology of IncQ and IncQ-Like Plasmids

Rawlings

Tietze

2001

Microbiol Mol Biol Rev

136

124

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“…R1162 is a member of a closely related group (Grinter and Barth, 1976) of small, multicopy plasmids that can be promiscuously transferred between unrelated species of bacteria by conjugation (Kreps et al, 1990;Gormley and Davies, 1991;Zinchenko et al, 1984). Three plasmidencoded proteins are required for conjugal mobilization; two of these, MobA and MobC, assemble at the origin of transfer (oriT ) to form a complex called the relaxosome (Scherzinger et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer

Zhang

Meyer

1997

Molecular Microbiology

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SummaryThe frequency of conjugal mobilization of plasmid R1162 is decreased approximately 50-fold if donor cells lack MobC, one of the plasmid-encoded proteins making up the relaxosome at the origin of transfer (oriT ). The absence of MobC has several different effects on oriT DNA. Site-and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA.

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“…The small (8,684-bp), broad-host-range plasmid R1162, and other members of the IncQ group, can be conjugally mobilized into a diverse group of organisms (7,18). Three R1162-encoded genes (mobA, mobB, and mobC) and an origin of transfer (oriT), all compactly organized within a 4,400-bp region of the plasmid (Fig.…”

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confidence: 99%

The primase of broad-host-range plasmid R1162 is active in conjugal transfer

Henderson

Meyer

1996

J Bacteriol

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The broad-host-range plasmid R1162 is conjugally mobilized at high frequency by the IncP-1 plasmid R751 but is poorly mobilized by pOX38, a derivative of the F factor. In both cases, the origin of transfer (oriT) and the Mob proteins of R1162 are required, indicating that these plasmids are mobilized by similar mechanisms. R1162 encodes a primase, essential for vegetative replication of the plasmid, that is made both as a separate protein and as the carboxy-terminal domain of MobA, one of the R1162 mobilization proteins (P. Scholz, V. Haring, B. Wittman-Liebold, K. Ashman, M. Bagdasarian, and E. Scherzinger, Gene 75:271-288, 1989). When R751 is the mobilizing vector, the primase is not required for mobilization of plasmids containing cloned mob-oriT R1162 DNA. However, detectable mobilization of such plasmids by pOX38 requires both the primase and its cognate initiation site, oriented for synthesis of the complement to the transferred strand. The long form of the primase is required for optimal transfer: R1162 replicons lacking this form also are not transferred detectably by pOX38 and are less well mobilized by R751. The distance between oriT and the primase initiation site affects the frequency of mobilization, and this effect is polar in the direction of transfer. Our results indicate that the R1162 primase is active in mobilization of R1162 and suggest that the MobA-linked form is an adaptation increasing its effectiveness during transfer.

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Conjugative transfer and autonomous replication of a promiscuous IncQ plasmid in the cyanobacterium Synechocystis PCC 6803

Cited by 50 publications

References 31 publications

Comparative Biology of IncQ and IncQ-Like Plasmids

Comparative Biology of IncQ and IncQ-Like Plasmids

The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer

The primase of broad-host-range plasmid R1162 is active in conjugal transfer

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