Conjugative multiple-antibiotic resistance plasmids in <i>Escherichia coli</i>
 isolated from environmental waters contaminated by human faecal wastes

Laroche-Ajzenberg, E.; Ribeiro, Angela Flores; Bodilis, Josselin; Riah, W.; Buquet, Sylvaine; Chaftar, Naouel; Pawlak, Barbara

doi:10.1111/jam.12691

Cited by 14 publications

(7 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, to the extent that non-ExPEC antimicrobial-resistant isolates contain transmissible resistance elements, they also may pose a threat, since if they were to be acquired during restroom use, they conceivably could transfer their resistance elements to a restroom user's (antibiotic-susceptible) endogenous intestinal Ex-PEC strains (42,43).…”

Section: Discussionmentioning

confidence: 99%

Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area

Mohamed

Owens

Gajewski

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

How extraintestinal pathogenic Escherichia coli (ExPEC) and antimicrobial-resistant E. coli disseminate through the population is undefined. We studied public restrooms for contamination with E. coli and ExPEC in relation to source and extensively characterized the E. coli isolates. For this, we cultured 1,120 environmental samples from 56 public restrooms in 33 establishments (obtained from 10 cities in the greater Minneapolis-St. Paul, MN, metropolitan area in 2003) for E. coli and compared ecological data with culture results. Isolates underwent virulence genotyping, phylotyping, clonal typing, pulsed-field gel electrophoresis (PFGE), and disk diffusion antimicrobial susceptibility testing. Overall, 168 samples (15% from 89% of restrooms) fluoresced, indicating presumptive E. coli: 25 samples (2.2% from 32% of restrooms) yielded E. coli isolates, and 10 samples (0.9% from 16% of restrooms) contained ExPEC. Restroom category and cleanliness level significantly predicted only fluorescence, gender predicted fluorescence and E. coli, and feces-like material and toilet-associated sites predicted all three endpoints. Of the 25 E. coli isolates, 7 (28%) were from phylogenetic group B2(virulence-associated), and 8 (32%) were ExPEC. ExPEC isolates more commonly represented group B2 (50% versus 18%) and had significantly higher virulence gene scores than non-ExPEC isolates. Six isolates (24%) exhibited >3-class antibiotic resistance, 10 (40%) represented classic human-associated sequence types, and one closely resembled reference human clinical isolates by pulsed-field gel electrophoresis. Thus, E. coli, ExPEC, and antimicrobial-resistant E. coli sporadically contaminate public restrooms, in ways corresponding with restroom characteristics and within-restroom sites. Such restroom-source E. coli strains likely reflect human fecal contamination, may pose a health threat, and may contribute to population-wide dissemination of such strains. E scherichia coli is a major cause of urinary tract and other extraintestinal infections, resulting in considerable morbidity, mortality, and costs (1). Most such infections are caused by distinctive E. coli strains called extraintestinal pathogenic E. coli (Ex-PEC) because of their enhanced ability to invade and cause disease at extraintestinal sites (2). Such strains' main reservoir is the human intestinal tract, where they usually reside harmlessly as longterm colonizers (3). ExPEC strains can be distinguished from other E. coli strains by their extensive virulence gene repertoire and primarily group B2 phylogenetic background (2).Dissemination of virulent and antimicrobial-resistant E. coli clones through the human population has been recognized recently as an important contributor to the overall burden of Ex-PEC-associated disease (4-7). However, the mechanisms of such clonal dissemination remain undefined. Flush toilets create microdroplets containing viable bacteria (8, 9), and both public and private restrooms have been shown to be variably contaminated with human-source bacteri...

show abstract

Section: Discussionmentioning

confidence: 99%

Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area

Mohamed

Owens

Gajewski

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Similarly, strA-strB , aphA1 and aacC 2 genes were also found to be plasmid-mediated and transferrable. Previous studies have indicated that conjugative plasmids were highly transferable and played a key role in conferring a multi-resistance phenotype to waterborne E. coli [14, 20, 52, 53].…”

Section: Resultsmentioning

confidence: 99%

Public health implications of plasmid-mediated quinolone and aminoglycoside resistance genes in Escherichia coli inhabiting a major anthropogenic river of India

et al. 2022

View full text Add to dashboard Cite

Presence of antimicrobial resistance (AMR) genes in Escherichia coli inhabiting anthropogenic rivers is an important public health concern because plasmid-mediated AMR genes can easily spread to other pathogens by horizontal gene transfer. Besides β-lactams, quinolones and aminoglycosides are the major antibiotics against E. coli. In the present study, we have investigated the presence of plasmid-mediated quinolone resistance (PMQR) and aminoglycoside resistance genes in E. coli isolated from a major river of northern India. Our results revealed that majority of the strains were phenotypically susceptible for fluoroquinolones and some aminoglycosides like amikacin, netilmicin, tobramycin and gentamicin. However, 16.39% of the strains were resistant for streptomycin, 8.19% for kanamycin and 3.30% for gentamicin. Of the various PMQR genes investigated, only qnrS1 was present in 24.59% of the strains along with ISEcl2. Aminoglycoside-resistance genes like strA-strB were found to be present in 16.39%, aphA1 in 8.19% and aacC2 in only 3.30% of the strains. Though, no co-relation was observed between phenotypic resistance for fluorquinolones and presence of PMQR genes, phenotypic resistance for streptomycin, kanamycin and gentamicin exactly co-related with the presence of the genes strA-strB, aphA1 and aacC2, respectively. Moreover, all the AMR genes discerned in aquatic E. coli were found to be situated on conjugative plasmids and, thus easily transferrable. Our study accentuates the importance of routine surveillance of urban rivers to curtail the spread of AMR genes in aquatic pathogens.

show abstract

“…A study conducted by Stoll et al [ 39 ] in Germany and Australia revealed a high resistance rate in E. coli isolates recovered from surface water samples that were resistant against ampicillin and tetracycline, and our result is in accordance with their report. A high percentage of the phenotypic resistance observed in the E. coli isolated could either be from the origin of WWTP or agricultural waste (poultry droppings), as most of the final effluents have been discharged into water bodies [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Pathogenic Escherichia coli Strains Recovered from Selected Aquatic Resources in the Eastern Cape, South Africa, and Its Significance to Public Health

Ebomah

Adefisoye

Okoh

2018

IJERPH

View full text Add to dashboard Cite

The prevalence of pathogenic microorganisms, as well as the proliferation of antimicrobial resistance, pose a significant threat to public health. However, the magnitude of the impact of aquatic environs concerning the advent and propagation of resistance genes remains vague. Escherichia coli (E. coli) are widespread and encompass a variety of strains, ranging from non-pathogenic to highly pathogenic. This study reports on the incidence and antibiotic susceptibility profiles of E. coli isolates recovered from the Nahoon beach and its canal waters in South Africa. A total of 73 out of 107 (68.2%) Polymerase chain reaction confirmed E. coli isolates were found to be affirmative for at least one virulence factor. These comprised of enteropathogenic E. coli 11 (10.3%), enteroinvasive E. coli 14 (13.1%), and neonatal meningitis E. coli 48 (44.9%). The phenotypic antibiogram profiles of the confirmed isolates revealed that all 73 (100%) were resistant to ampicillin, whereas 67 (91.8%) of the pathotypes were resistant to amikacin, gentamicin, and ceftazidime. About 61 (83.6%) and 51 (69.9%) were resistant to tetracycline and ciprofloxacin, respectively, and about 21.9% (16) demonstrated multiple instances of antibiotic resistance, with 100% exhibiting resistance to eight antibiotics. The conclusion from our findings is that the Nahoon beach and its canal waters are reservoirs of potentially virulent and antibiotic-resistant E. coli strains, which thus constitute a potent public health risk.

show abstract

Conjugative multiple-antibiotic resistance plasmids in Escherichia coli isolated from environmental waters contaminated by human faecal wastes

Abstract: These results highlight the key role played by plasmids in the multi-resistance phenotype of faecal bacteria and the diversity of these genetic structures. Contaminated water, especially accidentally contaminated drinking water, could be a path back to humans for these plasmids.

Cited by 14 publications

References 99 publications

Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area

Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area

Public health implications of plasmid-mediated quinolone and aminoglycoside resistance genes in Escherichia coli inhabiting a major anthropogenic river of India

Pathogenic Escherichia coli Strains Recovered from Selected Aquatic Resources in the Eastern Cape, South Africa, and Its Significance to Public Health

Contact Info

Product

Resources

About