Conjugative DNA-transfer in Streptomyces , a mycelial organism

Thoma, Lina; Muth, Günther

doi:10.1016/j.plasmid.2016.09.004

Cited by 27 publications

(26 citation statements)

References 77 publications

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“…The first one, which is widely distributed, is similar to that present in Gram-negative bacteria, where a single stranded DNA is transferred through a T4SS (Goessweiner-Mohr et al, 2014;Li et al, 2013;Wisniewski and Rood, 2017). In the second one, present in Streptomyces, the double stranded plasmid molecule is transferred through the walls of the hyphae, suggesting that conventional Mpf genes are not required, indicating important differences in the transfer mechanisms (Thoma and Muth, 2016). In a recent review by Thoma et al (2016), the conjugative transfer in Streptomyces was shown to require a DNA-translocase, namely TraB.…”

Section: Introductionmentioning

confidence: 98%

“…In the second one, present in Streptomyces, the double stranded plasmid molecule is transferred through the walls of the hyphae, suggesting that conventional Mpf genes are not required, indicating important differences in the transfer mechanisms (Thoma and Muth, 2016). In a recent review by Thoma et al (2016), the conjugative transfer in Streptomyces was shown to require a DNA-translocase, namely TraB. TraB resembles a protein involved in chromosome segregation during cell division, FtsK (Sepulveda et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of conjugative transfer of plasmids and integrative conjugative elements

Bañuelos-Vazquez

Tejerizo

Brom

2017

Plasmid

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Bañuelos-Vazquez

Tejerizo

Brom

2017

Plasmid

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“…and closely related relatives, but clc loci were not evident in the genomes of less‐related Actinobacteria such as Mycobacterium or Corynebacterium spp. (Thoma and Muth, ). The occurrence of clc sequences in Actinobacterial chromosomes seems to be correlated with the host range of Streptomyces ‐like plasmids (Thoma and Muth, ).…”

Section: Interaction Between Trab and Cltmentioning

confidence: 99%

“…(Thoma and Muth, ). The occurrence of clc sequences in Actinobacterial chromosomes seems to be correlated with the host range of Streptomyces ‐like plasmids (Thoma and Muth, ).…”

Section: Interaction Between Trab and Cltmentioning

confidence: 99%

“…Streptomyces conjugative plasmids typically encode several spd genes, which contribute to intramycelial spreading and thus pock size. Spd proteins do not show sequence similarity to functionally characterized proteins in other bacteria; moreover, most Spd proteins are not even conserved among Streptomyces plasmids (Thoma and Muth, ). An exception is the conserved SpdB2 protein (Tiffert et al ., ; Thoma and Muth, ).…”

Section: Intramycelial Spread In the Recipientmentioning

confidence: 99%

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Spreading the news about the novel conjugation mechanism in Streptomyces bacteria

Pettis

2018

Environ Microbiol Rep

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The hallmark of mycelial spore-forming bacteria of the genus Streptomyces is their prolific production of antibiotics and other bioactive secondary metabolites as part of a complex morphological and physiological developmental program. They are further distinguished by a conjugation mechanism that differs substantially from the single-strand mode of DNA transfer via Type IV secretion, which is exhibited by numerous unicellular Gram-negative and Gram-positive bacteria. At the crux of the novel intermycelial transfer event in Streptomyces spp. is a membrane pore composed of a single plasmid protein (TraB), which also functions as an FtsK-like DNA pump driven by the energy of ATP hydrolysis. TraB binds to specific 8-mer repeats within the non-coding clt plasmid transfer locus and the DNA is then translocated intercellularly in double-strand form. TraB also translocates chromosomal DNA most likely by binding to 8-mer clc sequences (clt-like chromosomal sequences) distributed throughout streptomycete chromosomes. In the recipient, plasmids are dispersed through septal crosswalls apparently by a multiprotein complex comprising TraB and plasmid Spd proteins. Continued rounds of such intramycelial spreading distribute plasmids well beyond the initial entrance point during the time prior to cell differentiation and sporulation.

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