Conjugations With Glutathione. The Enzymic Conjugation of Some Chlorocyclohexenes

Abstract

1. alpha-3,4,5,6-Tetrachlorocyclohex-1-ene and gamma-2,3,4,5,6-pentachlorocyclohex-1-ene are conjugated with glutathione in vitro by a rat-liver enzyme that is probably glutathione S-aryltransferase. 2. Chlorocyclohexane and the alpha-, beta-, gamma- and delta-isomers of hexachlorocyclohexane were not substrates for rat-liver glutathione S-aryltransferase. 3. Glutathione-S-aryltransferase activity was present in tissue preparations of houseflies of insecticide-resistant and -susceptible strains. More activity was found in a dieldrin-resistant strain of houseflies fed on dieldrin than in either a dieldrin-resistant strain not fed on dieldrin or a control strain of dieldrin-susceptible houseflies. 4. Housefly soluble supernatant preparations converted S-(2-chloro-4-nitrophenyl)glutathione into the corresponding cysteine and mercapturic acid derivatives.

Degradation of lindane by cell-free preparations of Clostridium sphenoides

“…This requirement for GSH suggests that a glutathione conjugation may be involved in y-HCH degradation by C. sphenoides. Sims and Grover (8) and Ishida and Dahm (5) have reported similar glutathionedependent, chlorinated hydrocarbon-degrading systems in rat liver and housefly homogenates, respectively.…”

Residues of Pesticides and Other Foreign Chemicals in Foods and Feeds / Rückstände von Pesticiden und anderen Fremdstoffen in Nahrungs- und Futtermitteln

“…Rate of metabolism of BHC under in vitro conditions does not correlate with levels of resistance (SIMS andGROVER 1965, ISHIDA andDAHM 1965 a), thus metabolism of BHC might be a resistance mechanism of minor importance. Therefore, much more works should be done on biochemistry and physiology of BHCresistance to correlate to such a complicated genetic system.…”