Conjugation polymer nanobelts: a novel fluorescent sensing platform for nucleic acid detection †

Abstract

In this article, we report on the facile and rapid synthesis of conjugation polymer poly(p-phenylenediamine) nanobelts (PNs) via room temperature chemical oxidation polymerization of p-phenylenediamine monomers by ammonium persulfate in aqueous medium. We further demonstrate the proof-of-concept that PNs can be used as an effective fluorescent sensing platform for nucleic acid detection for the first time. The general concept used in this approach lies in the facts that the adsorption of the fluorescently labeled single-stranded DNA probe by PN leads to substantial fluorescence quenching, followed by specific hybridization with the complementary region of the target DNA sequence. This results in desorption of the hybridized complex from PN surface and subsequent recovery of fluorescence. We also show that the sensing platform described herein can be used for multiplexing detection of nucleic acid sequences.

Candidate gene mapping identifies genomic variations in the fire blight susceptibility genes HIPM and DIPM across the Malus germplasm

“…The variants were annotated using the coding and protein sequences of HIPM and DIPM genes from the GDDH13 v1.1 43 genome. In addition, the gene annotations were obtained from the GDDH13 v1.1 43 genome to annotate variants using the ANNOVAR program 46 .…”

Biomimetic Assembly of a Polydopamine Layer on Graphene as an Electron Gate for Fluorescent MicroRNA Detection in Living Cells

“…Recently, a number of fluorescence‐based miRNA detection systems have been developed, among which fluorescence resonance energy transfer (FRET) or the quenching mechanism have attracted much attention. FRET or the quenching mechanism have been used in DNA detection for decades and, to date, many nanostructures have been developed as efficient nanoquenchers for fluorometric DNA detection, Similarly, these structures can also be applied to miRNA detection in solution and modified to improve cellular uptake or other properties.…”

Diaminium salt as reactive amphiphile for the synthesis of poly(m-phenylenediamine) and paraffin microencapsulation

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