Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay

Khramtsov, Pavel; Kropaneva, Maria; Бызов, И. В.; Minin, Artem S.; Мысик, А. А.; Тимганова, В. П.; Бочкова, М. С.; Уймин, М. А.; Заморина, С. А.; Yermakov, A. Ye.; Rayev, M. B.

doi:10.1016/j.colsurfb.2019.01.009

Cited by 21 publications

(9 citation statements)

References 40 publications

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“…Conjugates of magnetic nanoparticles with proteins were synthesized using the following strategy. Fe@C Powder with a mean size of approximately 6 nm [33] was aminated. The aminated iron-carbon nanoparticles (Fe@C-NH 2 ) were added to aqueous protein solutions.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, we demonstrated that variations in pH could be applied to tune the size of BSA-coated nanoclusters [33]. Here, we investigated the influence of coating conditions (pH, ionic strength, protein-to-nanoparticle mass ratio and sonication time) on the interaction between different proteins (casein, BSA, and gelatins) and Fe@C-NH 2 to obtain protein-coated nanoclusters of the desired size.…”

Section: Resultsmentioning

confidence: 99%

“…In our experiments, the size of nanoclusters resulted from a trade-off between the aggregation of Fe@C-NH 2 at pH > 4 and the protein stabilization of Fe@C-NH 2 aggregates. Fe@C-NH 2 is stable at pH 4 [33] (see the zeta potential of Fe@C-NH 2 in Figure S2) and aggregates slowly at pH 5 and 9, while at neutral pH, immediate aggregation occurs. Generally, a slight increase in size was observed when the coating was performed at pH 6–8; this effect was more pronounced for gelatin B (Figures S3–S6).…”

Section: Resultsmentioning

confidence: 99%

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Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

et al. 2019

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The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine. Natural polymers, including proteins, are prospective coatings capable of increasing the stability, biocompatibility, and transverse relaxivity (r2) of magnetic nanoparticles. In this work, we functionalized the nanoclusters of carbon-coated iron nanoparticles with four proteins: bovine serum albumin, casein, and gelatins A and B, and we conducted a comprehensive comparative study of their properties essential to applications in biosensing. First, we examined the influence of environmental parameters on the size of prepared nanoclusters and synthesized protein-coated nanoclusters with a tunable size. Second, we showed that protein coating does not significantly influence the r2 relaxivity of clustered nanoparticles; however, the uniform distribution of individual nanoparticles inside the protein coating facilitates increased relaxivity. Third, we demonstrated the applicability of the obtained nanoclusters in biosensing by the development of a nuclear-magnetic-resonance-based immunoassay for the quantification of antibodies against tetanus toxoid. Fourth, the protein coronas of nanoclusters were studied using SDS-PAGE and Bradford protein assay. Finally, we compared the colloidal stability at various pH values and ionic strengths and in relevant complex media (i.e., blood serum, plasma, milk, juice, beer, and red wine), as well as the heat stability, resistance to proteolytic digestion, and shelf-life of protein-coated nanoclusters.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

et al. 2019

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“…Because carbon-based shell containing few layers of graphene is now recognized as a novel platform for both drug and gene delivery systems (DGDS) in nanomedicine (Bamburowicz-Klimkowska et al 2019), the CEINS nanomaterials composed of the iron (Fe) core covered with carbon shells are now expected to be the perfect targeting magnetic core-shelltype negative contrast candidate when decorated with surface ligands in MRI. This feature has opened up some new exciting avenues for bioengineering carboncoated magnetic nanomaterials as potent theranostic agents in preclinical MRI studies (Bae et al 2012;Lee et al 2014;Khramtsov et al 2019;Park et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive magnetic resonance characteristics of carbon-encapsulated iron nanoparticles: a new frontier for the core-shell–type contrast agents

et al. 2020

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The development of carbon-encapsulated iron nanoparticles (CEINS) is of considerable interest in many areas of cancer nanotechnology, ranging from basic tumor biology to early detection and treatment of cancer. To meet these challenges, the present study was undertaken to determine the magnetic and relaxometric performance of CEINS used as a new contrast agent for magnetic resonance imaging (MRI) in preclinical phantom models. CEIN samples were synthesized using a carbon arc discharge route, and the as-synthesized nanoparticles were purified and functionalized with surface acidic groups. The presence of various Fe-bearing metallic phases reflecting the net magnetic properties of CEINS was characterized by powder X-ray diffraction (XRD), thermogravimetry (TGA), and vibrating sample magnetometry. The morphological and surface chemistry features were characterized by electron microscopy (transmission electron microscopy [TEM] and scanning electron microscopy [SEM]), Raman spectroscopy, and Fourier transform-infrared (FT-IR) spectroscopy. The textural properties of CEINS, including porosity, surface total charge density, and zeta potentials, were also measured. The as-synthesized different CEIN samples were finally examined as a potent MRI contrast drug candidate. Magnetic resonance relaxation measurements were performed in bovine gelatin-based phantom models by using a 1.5-T MRI scanner equipped with a standard radiofrequency "birdcage" type head coil. To obtain data, T1-and T2-weighted MR images were acquired using the inversion recovery spin echo (SE) and the SE protocol with multiple time of echo (TE), respectively. Chemical characterization showed similarity in morphology and textural properties between assynthesized CEINS, purified CEINS, and CEINS functionalized with acidic groups. The as-synthesized CEINS had significantly higher Fe content and higher saturation magnetization. The analysis of the

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“…They reported a successful synthesize and characterization of carbon-encapsulated Fe nanoparticles [10]. Khramtsov et al further developed carbon coated Fe nanoparticles to optimize its conjugation with streptavidin and monoclonal antibodies [11]. Apart from inorganic coatings, organic polymer coatings are feasible for Fe nanoparticles as well.…”

Section: Introductionmentioning

confidence: 99%

Investigations on Forming Ether Coated Iron Nanoparticle Materials by First-Principle Calculations and Molecular Dynamic Simulations

et al. 2019

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The mechanism of coating effects between ether molecules and iron (Fe) nanoparticles was generally estimated using first-principle calculations and molecular dynamic (MD) simulations coupling with Fe (110) crystal layers and sphere models. In the present work, the optimized adsorption site and its energy were confirmed. The single sphere model in MD simulations was studied for typical adsorption behaviors, and the double sphere model was built to be more focused on the gap impact between two particles. In those obtained results, it is demonstrated that ether molecules were prone to be adsorbed on the long bridge site of the Fe (110) crystal while comparing with other potential sites. Although the coating was not completely uniform at early stages, the formation of ether layer ended up being equilibrated finally. Accompanied with charge transfer, those coated ether molecules exerted much binding force on the shell Fe atoms. Additionally, when free ether molecules were close to the gap between two nanoparticles, they were found to come under double adsorption effects. Although this effect might not be sufficient to keep them adsorbed, the movement of these ether molecules were hindered to some extent.

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Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay

Cited by 21 publications

References 40 publications

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

Comprehensive magnetic resonance characteristics of carbon-encapsulated iron nanoparticles: a new frontier for the core-shell–type contrast agents

Investigations on Forming Ether Coated Iron Nanoparticle Materials by First-Principle Calculations and Molecular Dynamic Simulations

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