Conjugation In Saccharomyces Cerevisiae

Cross, Frederick R.

doi:10.1146/annurev.cellbio.4.1.429

Cited by 74 publications

(104 citation statements)

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“…In the remaining 15% of mutant cells, a spindle of short length similar to that normally seen early in G2 was aberrantly positioned entirely within the bud ( Figure 2C2). The bud was easily distinguished from the mother cell, which had retained the "shmoo" form acquired during mating pheromone arrest (Cross et al, 1988). A further indication of mitotic abnormality was signaled by the position of chromosomal DNA, as seen by DAPI staining.…”

Section: Cloning and Sequencing Of The Esp1 Locusmentioning

confidence: 93%

Requirement for ESP1 in the nuclear division of Saccharomyces cerevisiae.

McGrew

Goetsch

Byers

et al. 1992

MBoC

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Mutations in the ESP1 gene of Saccharomyces cerevisiae disrupt normal cell-cycle control and cause many cells in a mutant population to accumulate extra spindle pole bodies. To determine the stage at which the esp1 gene product becomes essential for normal cell-cycle progression, synchronous cultures of ESP1 mutant cells were exposed to the nonpermissive temperature for various periods of time. The mutant cells retained viability until the onset of mitosis, when their viability dropped markedly. Examination of these cells by fluorescence and electron microscopy showed the first detectable defect to be a structural failure in the spindle. Additionally, flow cytometric analysis of DNA content demonstrated that massive chromosome missegregation accompanied this failure of spindle function. Cytokinesis occurred despite the aberrant nuclear division, which often resulted in segregation of both spindle poles to the same cell. At later times, the missegregated spindle pole bodies entered a new cycle of duplication, thereby leading to the accumulation of extra spindle pole bodies within a single nucleus. The DNA sequence predicts a protein product similar to those of two other genes that are also required for nuclear division: the cut1 gene of Schizosaccharomyces pombe and the bimB gene of Aspergillus nidulans.

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Section: Cloning and Sequencing Of The Esp1 Locusmentioning

confidence: 93%

Requirement for ESP1 in the nuclear division of Saccharomyces cerevisiae.

McGrew

Goetsch

Byers

et al. 1992

MBoC

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“…On the basis of similarity to the S. cerevisiae MFα proteins, the P. chrysogenum Pcppg1 gene was predicted to produce a decapeptide pheromone of sequence KWCGHIGQGC, expected to bind to the cognate PcPRE2 receptor protein. And indeed, S. cerevisiae wild-type cells (ScSTE2p) or yeasts heterologously expressing PcPRE2 exhibited polarized growth, leading to pear-shaped forms (shmoos) of unconjugated haploid cells, in response to either the native S. cerevisiae α-factors or the synthetic decapeptide pheromone PcPPG1, respectively (35) (Fig. 4A).…”

Section: Functionality Of the P Chrysogenum Pheromone And Pheromonementioning

confidence: 95%

Sexual reproduction and mating-type–mediated strain development in the penicillin-producing fungus Penicillium chrysogenum

Böhm

Hoff

O’Gorman

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

119

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Penicillium chrysogenum is a filamentous fungus of major medical and historical importance, being the original and present-day industrial source of the antibiotic penicillin. The species has been considered asexual for more than 100 y, and despite concerted efforts, it has not been possible to induce sexual reproduction, which has prevented sexual crosses being used for strain improvement. However, using knowledge of mating-type (MAT) gene organization, we now describe conditions under which a sexual cycle can be induced leading to production of meiotic ascospores. Evidence of recombination was obtained using both molecular and phenotypic markers. The identified heterothallic sexual cycle was used for strain development purposes, generating offspring with novel combinations of traits relevant to penicillin production. Furthermore, the MAT1-1-1 mating-type gene, known primarily for a role in governing sexual identity, was also found to control transcription of a wide range of genes with biotechnological relevance including those regulating penicillin production, hyphal morphology, and conidial formation. These discoveries of a sexual cycle and MAT gene function are likely to be of broad relevance for manipulation of other asexual fungi of economic importance.sexual recombination | secondary metabolism | ascomycete

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“…For instance, before START, yeast are competent to respond to mating pheromone, initiating an alternative developmental program that will end with fusion to a partner to form a diploid cell (reviewed in ref. 15). After START, yeast will no longer respond to mating pheromone.…”

Section: Introductionmentioning

confidence: 99%