Conjugation and chromosome transfer in Rhodopseudomonas sphaeroides mediated by W and P group plasmids

Tucker, William T.

doi:10.1016/0378-1097(79)90027-2

Cited by 4 publications

(7 citation statements)

References 11 publications

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“…Plasmids of the P and W incompatibility groups are well known for their ability to transfer to many bacterial genera and have been used to mediate genetic exchange in various bacteria (Dreyfus & Iglewski, 1985;Tatra & Goodwin, 1983;Tucker & Pemberton, 1979). Our results show that the conjugation genes of a P plasmid (RK2) can transfer pRKTV5 and pUW964 to P .…”

Section: Discussionsupporting

confidence: 51%

Transfer and Properties of Some Natural and Suicide Replicons in Pasteurella multocida

Nnalue

Stocker²

1989

Microbiology

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3345We tested the transfer of several plasmids and transposons from Escherichia coli to Pasteurella multocida by filter mating. Two plasmids, pRKTV5 (pRK2013 : :Tn7) and pUW964 (pRKTV5 : : TnS), were derived from pRK2013 -a narrow-host-range plasmid with the broadhost-range IncP conjugation genes. Most P. multocida transconjugants obtained with pRKTV5 had Tn7 insertions in the chromosome but some had insertions of the whole plasmid. By contrast, all the transconjugants obtained with pUW964 had insertions of this plasmid or a deleted variant. pU W964 mediated low-frequency transfer of Tn7 or chromosomal markers between P . multocida strains. Broad-host-range IncP plasmid RP4 (RK2) did not yield selectable transconjugants in P . multocida but two plasmids derived by Tn5 insertion into a kanamycinsensitive derivative of RP4 did yield transconjugants. pSUPlOl1, a narrow-host-range pl5A replicon with the RP4 mob region allowing mobilization by the IncP conjugation genes also yielded transconjugants while several other plasmids tested did not transfer markers to P . multocida.

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Section: Discussionsupporting

confidence: 51%

Transfer and Properties of Some Natural and Suicide Replicons in Pasteurella multocida

Nnalue

Stocker²

1989

Microbiology

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“…Bacterial and bacteriophage strains. R. sphaeroides RS6143 (ade-3 car-i) has been described previously (20). Strain RS6143(R409) was isolated by picking from the center of a turbid R49 plaque grown on RS6143 and purified twice for single colonies.…”

Section: Methodsmentioning

confidence: 99%

“…The photosynthetic bacterium Rhodopseudomonas sphaeroides is now being used in attempts to analyze the genetic aspects of bacterial photosynthesis. A number of workers have reported that Inc P-1 (10,15,20) and Inc W (20) group R plasmids are stably maintained in this organism and have been used as cloning vectors (S. Kaplan, personal communication). A transformation system for R. sphaeroides would eliminate some of the problems arising from the need to transform cloned R. sphaeroides DNA into E. coli before its reintroduction into R. sphae-roides with conjugative plasmids.…”

mentioning

confidence: 99%

Transformation of Rhodopseudomonas sphaeroides with deoxyribonucleic acid isolated from bacteriophage R phi 6P

Tucker¹,

Pemberton²

1980

J Bacteriol

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The transfornation of the photosynthetic bacterium Rhodopseudomonas sphaeroides with the circular genome of the penicillinase-encoding, temperate bacteriophage R46P was demonstrated. The transformation was dependent on the infection of the recipient by another, apparently closely related, temperate bacteriophage, R49. Optimum transformation occurred in the recipient cells already lysogenic for R49 when superinfected with that bacteriophage at multiplicities of infection between 1 and 10 R49 particles per recipient cell.

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“…Although specialized transduction and transformation have been reported for R. sphaeroides (20,27,29) and a type of generalized transduction has been discovered in the closely related Rhodopseudomonas capsulata (15,24,31), naturally occurring conjugation and chromosome transfer systems have not been detected among members of the family Rhodospirillaceae (21). Fortunately, a number of broad-host-range R plasmids are freely transmissible to R. sphaeroides from E. coli (17,18,23,28). With the exception ofR68.45 (23), these plasmids promote chromosome transfer at low frequencies (28) and thus are of limited value in mapping the genome of this bacterium.…”

mentioning

confidence: 99%

“…Fortunately, a number of broad-host-range R plasmids are freely transmissible to R. sphaeroides from E. coli (17,18,23,28). With the exception ofR68.45 (23), these plasmids promote chromosome transfer at low frequencies (28) and thus are of limited value in mapping the genome of this bacterium.…”

mentioning

confidence: 99%

High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501

Pemberton¹,

Bowen²

1981

J Bacteriol

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Insertion of the mercury resistance transposon Tn501 into broad-host-range plasmid RP1 greatly enhanced the ability of this plasmid to promote chromosome transfer in the photosynthetic bacterium Rhodopseudomonas sphaeroides. Compared with the wild-type RP1, which produced less than 10(-8) recombinants per donor cell, RP1::Tn501 produced between 10(-3) and 10(-7) recombinants per donor cell depending upon the marker selected. Plasmid RP1::Tn501 promoted polarized transfer of the chromosome from one or perhaps two origins on the chromosome, giving rise to two linkage groups. All of the biosynthetic and antibiotic resistance genes that have been mapped, including those involved in photosynthesis, occur on one or another of these linkage groups.

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Conjugation and chromosome transfer in Rhodopseudomonas sphaeroides mediated by W and P group plasmids

Cited by 4 publications

References 11 publications

Transfer and Properties of Some Natural and Suicide Replicons in Pasteurella multocida

Transfer and Properties of Some Natural and Suicide Replicons in Pasteurella multocida

Transformation of Rhodopseudomonas sphaeroides with deoxyribonucleic acid isolated from bacteriophage R phi 6P

High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501

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