Conjugated Polymers for Two‐Photon Live Cell Imaging

Li, Shuang; Jiang, Xiaofang; Xu, Qing‐Hua

doi:10.1002/9783527342747.ch6

Cited by 4 publications

(3 citation statements)

References 144 publications

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“…In general, CPs are highly hydrophobic, and thus, to realize these materials for in vivo biomedical applications requires significant improvement of their aqueous solubility. In this direction, the following two strategies have been considered [ 45 ]. The first one is formulating CPs into nanoparticles (CPNs) by using nanoengineering techniques or integrating it into amphiphilic block copolymer nanoassemblies using appropriate methods, such as the physical encapsulation method discussed above.…”

Section: Polymeric Nanoplatforms For Two-photon-assisted Imagingmentioning

confidence: 99%

Recent Advances in Biomedical Applications of Polymeric Nanoplatform Assisted with Two-Photon Absorption Process

et al. 2022

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Polymers are well-recognized carriers useful for delivering therapeutic drug and imaging probes to the target specified in the defined pathophysiological site. The functional drug molecules and imaging agents were chemically attached or physically loaded in the carrier polymer matrix via cleavable spacers. Using appropriate targeting moieties, these polymeric carriers (PCs) loaded with functional molecules were designed to realize target-specific delivery at the cellular level. The biodistribution of these carriers can be tracked using imaging agents with suitable imaging techniques. The drug molecules can be released by cleaving the spacers either by endogenous stimuli (e.g., pH, redox species, glucose level and enzymes) at the targeted physiological site or exogenous stimuli (e.g., light, electrical pulses, ultrasound and magnetism). Recently, two-photon absorption (2PA)-mediated drug delivery and imaging has gained significant attention because TPA from near-infrared light (700–950 nm, NIR) renders light energy similar to the one-photon absorption from ultraviolet (UV) light. NIR has been considered biologically safe unlike UV, which is harmful to soft tissues, cells and blood vessels. In addition to the heat and reactive oxygen species generating capability of 2PA molecules, 2PA-functionalized PCs were also found to be useful for treating diseases such as cancer by photothermal and photodynamic therapies. Herein, insights attained towards the design, synthesis and biomedical applications of 2PA-activated PCs are reviewed. In particular, specific focus is provided to the imaging and drug delivery applications with a special emphasis on multi-responsive platforms.

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Section: Polymeric Nanoplatforms For Two-photon-assisted Imagingmentioning

confidence: 99%

Recent Advances in Biomedical Applications of Polymeric Nanoplatform Assisted with Two-Photon Absorption Process

et al. 2022

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“…They usually have the advantages of a large Stokes shift, high quantum yield (Φ), excellent optical stability, and reasonable application prospects in the field of fluorescence imaging [13,14]. However, their targeting has been questioned, limiting further application [15,16,17], whereas many small organic molecules have exhibited a high performance at recognizing the targets [18,19]. Thus, we think it is possible to improve the TP properties of dyes by combining small molecules with CPs.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer

Zhai

Wang

et al. 2019

Polymers

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The calcium ion (Ca2+) isa highly versatile intracellular signal messenger regulating many different cellular functions. It is important to design probes with good fluorescence and two-photon (TP) active cross-sections (Φδ) to explore the concentration distribution of Ca2+. In this manuscript, a novel TP fluorescence calcium probe (BAPTAVP) with positive charges, based on the classical Ca2+ indicator of BAPTA (1,2-bis(2-aminophenoxy)-ethane-N,N,N’,N’-tetra acetic acid), and a conjugated polymer (PCBMB) with negative charges were designed and synthesized. The results from transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), and the zeta potential (ZP) showed that nanoparticles were obtained by the self-assembly of PCBMB and BAPTAVP. Moreover, the fluorescence properties of BAPTAVP were effectively improved by fluorescence resonance energy transfer (FRET) with PCBMB and attenuating the intramolecular charge transfer (ICT) after the addition of Ca2+. The quantum yield and Φδ of PCBMB-BAPTAVP increased by about four and six times in comparison to those of BAPTAVP, respectively. The TP fluorescence imaging experiments indicated that the PCBMB-BAPTAVP system could effectively detect Ca2+ in living cells with high sensitivity.

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“…Until now, the most explored PTAs mainly include inorganic nanomaterials (such as gold nanostructures, metal nanostructures, and carbon-based materials) and organic polymers (e.g., organic fluorophores and semiconductor polymers). − Compared with inorganic nanomaterials, organic PTAs exhibit superiority both in biodegradability and biocompatibility. Recently, conjugated polymers (CPs) and their corresponding nanoparticles (CPNs) with large π-conjugated backbones and versatile optical properties have emerged as outstanding candidates especially in biosensing, fluorescence imaging, antimicrobial, and cancer therapy because of high molar absorption coefficients and preferable photostability. − Over the past few years, enormous efforts have been made to fabricate new CPNs with NIR absorption via combination of multifarious donor and acceptor structures. , The most typical CPs used for PTT mainly include polypyrrole, polyaniline, and polythiophene derivatives. − However, the limited surface functional groups and difficult-to-adjust absorption wavelengths of these CPNs motivate researchers to develop new CPs.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanomaterials of Conjugated Polymers and Albumin for Precise Photothermal Therapy

Zhang

Lin

Wang

et al. 2018

ACS Appl. Mater. Interfaces

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Heretofore, conjugated polymers (CPs) attract considerable attention in photothermal therapy (PTT). Although various CPs with different structures have been reported, the suboptimal circulation persistence and biodistribution limit their efficacy in tumor treatment. Human serum albumin (HSA), an endogenous plasma protein, has been widely functioned as a carrier for therapeutic agents. Herein, we construct nanocomplex C16 pBDP@HSA nanoparticles (NPs) from hydrophobic 4,4-difluoro-4-bora-3a,4adiaza-s-indacene (BODIPY)-containing CPs and HSA, which exhibit robust stability in physiological conditions and excellent photothermal activity upon irradiation. The high photothermal conversion efficiency of 37.5%, higher than that of other reported PTT agents such as gold nanorods, phosphorus quantum dots, and 2D materials, results in the potent photocytotoxicity toward cancer cells. Simultaneously, C16 pBDP@HSA NPs' capabilities of near-infrared fluorescence and photoacoustic imaging can provide guidance to the PTT. The outstanding inhibition of tumor growth results from great photothermal activity, the benefited accumulation in tumor, and optimal timing of treatment. To the best of our knowledge, this is the first study which combines the BODIPY-based CPs and HSA in one nanostructure and finds application in cancer treatment. Moreover, this article also offers a new strategy for other insoluble macromolecules to explore more biomedical applications.

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Conjugated Polymers for Two‐Photon Live Cell Imaging

Cited by 4 publications

References 144 publications

Recent Advances in Biomedical Applications of Polymeric Nanoplatform Assisted with Two-Photon Absorption Process

Recent Advances in Biomedical Applications of Polymeric Nanoplatform Assisted with Two-Photon Absorption Process

Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer

Hybrid Nanomaterials of Conjugated Polymers and Albumin for Precise Photothermal Therapy

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