Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1)

Basiricò, Loredana; Morera, P.; Dipasquale, D.; Tröscher, A.; Serra, Andrea; Mele, Marcello; Bernabucci, U.

doi:10.3168/jds.2015-9787

Cited by 39 publications

(33 citation statements)

References 40 publications

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“…In our study, and in accordance with that previously reported by Szczechowiak et al , the increase in concentration of vaccenic acid could be favoured by the action of bioactive compounds present in the DGP which tend to inhibit the terminal steps of ruminal biohydrogenation, thus avoiding the formation of stearic acid (C18: 0) . The importance of vaccenic acid lies in the fact that this compound represents the substrate of the mammary gland Δ9‐desaturase, which is responsible for the production of at least 90% of rumenic acid, which is reported to have a relevant role in mammary gland functionality, because of its antioxidant activity that preserve bovine mammary epithelial cells from lipoperoxidation . Moreover, CLA are compounds with high biological value for humans, due to their potential activity in slowing the development of atherosclerosis, modulating the immune system and influencing glucose and lipid metabolism .…”

Section: Discussionsupporting

confidence: 91%

“…7 The importance of vaccenic acid lies in the fact that this compound represents the substrate of the mammary gland Δ9-desaturase, which is responsible for the production of at least 90% of rumenic acid, which is reported to have a relevant role in mammary gland functionality, because of its antioxidant activity that preserve bovine mammary epithelial cells from lipoperoxidation. 41 Moreover, CLA are compounds with high biological value for humans, due to their potential activity in slowing the development of atherosclerosis, 42 modulating the immune system 43 and influencing glucose and lipid metabolism. 44 The increased amount of PUFA and a parallel decrease of SFA content in GP+ samples of milk induced a reduction of the SFA/UFA ratio and, as a consequence, of the thrombogenic index.…”

Section: Discussionmentioning

confidence: 99%

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Chemical–nutritional quality and oxidative stability of milk and dairy products obtained from Friesian cows fed with a dietary supplementation of dried grape pomace

et al. 2019

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BACKGROUND The aim of the study was to evaluate the nutritional properties of milk and cheese obtained from Friesian cows fed with a diet supplemented with dried grape pomace, a by‐product of the oenological industry, which is very rich in polyphenols. This approach is inspired by the increasing interest in foods containing functional ingredients that may have beneficial effects on human health. During the testing period, analyses of the chemical and nutritional properties of milk and dairy products derived from it were performed; particular attention was given to the effect of cheese ripening on the oxidative stability and fermentation process, evaluating respectively the presence of malondialdehyde and γ‐aminobutyric acid. RESULTS Dietary enrichment with grape pomace did not affect the milk composition but induced modifications in the fatty acid profiles in both milk and cheese with an increase in concentration of linoleic acid, trans‐vaccenic acid, rumenic acid and total n‐6 fatty acids. Moreover, after 30 days of cheese ripening, an increased oxidative stability and an increased concentration of γ‐aminobutyric acid were found. CONCLUSIONS Our results indicated a general improvement in nutritional parameters of milk and related cheese obtained from Friesian cows that received the feeding enrichment with dried grape pomace. Further analysis should be performed to improve knowledge of the chemical and microbiological mechanisms at the source of these findings. © 2019 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Chemical–nutritional quality and oxidative stability of milk and dairy products obtained from Friesian cows fed with a dietary supplementation of dried grape pomace

et al. 2019

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“…Recently, Basiricò et al. (, ) showed an improved antioxidative status in bovine mammary epithelial cells. During the intramammary LPS challenge, plasma concentration of glucose increased in both treatment groups in agreement with earlier studies (Vernay et al., ; Waldron et al., ; Zarrin et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Not much research was carried out regarding CLA effects on the immune system of transition dairy cows. Supplementation of bovine mammary epithelial cells with CLA improved their oxidative status (Basiricò, Morera, Dipasquale, Tröscher, & Bernabucci, ; Basiricò et al., ). Dänicke et al.…”

Section: Introductionmentioning

confidence: 99%

Inflammatory and metabolic responses to an intramammary lipopolysaccharide challenge in early lactating cows supplemented with conjugated linoleic acid

Gross

Grossen-Rösti

Héritier

et al. 2017

Animal Physiology Nutrition

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Supplementation of dairy cows with trans-10, cis-12 conjugated linoleic acid (CLA) allows nutrient repartitioning despite an energy deficiency in early lactation, which might be a benefit for the immune system, too. In this study, we investigated potential nutrient sparing effects of CLA in early lactating cows with low plasma glucose concentrations exposed to an intramammary lipopolysaccharide (LPS) challenge. Fifteen multiparous Holstein cows were exposed to an intramammary LPS challenge in week 4 p.p. Eight cows (CLA) were supplemented daily with 70 g of lipid-encapsulated CLA (6.8 g trans-10, cis-12 and 6.6 g of the cis-9, trans-11 CLA isomer; CLA) and seven cows with 56 g of control fat (CON). Blood samples were obtained every 30 min along with rectal temperature, heart and respiratory rate, and milk samples were taken hourly until 10 hr after the LPS application. Plasma was analysed for concentrations of glucose, free fatty acids, beta-hydroxybutyrate (BHB), cortisol, insulin and glucagon. In milk, somatic cell count and activity of lactate dehydrogenase were determined. Initial plasma glucose concentration was lower in CLA than in CON. During the immunostimulation, CLA had higher glucose concentrations than CON, and BHB decreased distinctly in CLA, whereas CON cows maintained BHB concentration at a lower level. Body temperature in CLA increased earlier, the difference between peak and basal temperature was higher, and the decline thereafter occurred earlier. In conclusion, CLA supplementation of early lactating cows exposed to an intramammary LPS challenge affected local and systemic immune responses. We assume that CLA supplementation triggered glycogen storage. Cows supplemented with CLA provided more glucose and preferentially used BHB as an energy source during the immune response. The more intense metabolic and more concentrated endocrine responses support an immunomodulatory effect of CLA supplementation.

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“…Due to the high metabolic rate of the mammary epithelial cells during lactation, it can produce large amounts of ROS and lipid peroxides in vivo, especially in high-yielding dairy cows. If these excessive oxygen free radicals are doi: 10.17221/8851-CJAS not cleared in time, oxidative stress occurs when there is an imbalance between the production of ROS and the host antioxidant capabilities (Basirico et al 2015), and contributes to compromised immunity and increases incidence of diseases (Sordillo and Aitken 2009). Selenoprotein can scavenge hydrogen peroxide, lipid peroxide radicals and phospholipids, and protect tissues against oxidative damage (Bruzelius et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Effects of retinoic acid on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells in vitro

Jin¹,

Yan²,

Shi³

et al. 2016

CZECH J ANIM SCI

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The present study was conducted to examine the effects of retinoic acid (RA) on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells (BMEC) in vitro and to explore the antioxidative mechanisms of RA in the BMEC. The subconfluenced BMEC were divided into six treatments with six replicates per treatment and cultured in a Dulbecco's Modified Eagle's Medium/F12 media (10% fetal bovine serum, 5 µg/ml ovine prolactin, 10 ng/ml epidermal growth factor, 1 g/ml hydrocortisone, 0.5% insulin-transferrin-selenium) containing different levels of RA (0 (control), 0.05, 0.1, 0.2, 1 or 2 mg/ml) for 24 h. Addition of RA promoted the proliferation of BMEC, increased the activities of catalase, superoxide dismutase, total antioxidant capacity, glutathione peroxidase (GPX), thioredoxin reductase (TRXR), and the content of selenoprotein P (SELP) in a dose-dependent manner (P < 0.05). The optimal RA dose was 1 μg/ml. However, positive effect of RA tended to be suppressed when RA was increased to 2 μg/ml. The expressions of mRNA and protein of GPX in BMEC were up-regulated by RA in a quadratic dose-response relationship (P < 0.01), and the addition of 1 μg/ml RA showed the best effect. The mRNA expressions of TRXR1 and SELP as well as the protein expression of TRXR1 were higher at 1-2 μg/ml RA. These results suggested that RA promoted antioxidant function of BMEC by regulating the synthesis of selenoprotein including GPX, TRXR, and SELP in vitro. Keywords: vitamin A; antioxidant function; dairy cowsList of abbreviations: BMEC = bovine mammary epithelial cells, NO = nitric oxide, VA = vitamin A, GPX = glutathione peroxidase, TRXR = thioredoxin reductase, ROS = reactive oxygen species, MDA = malondialdehyde, SELP = selenoprotein P, RA = retinoic acid, ID = iodothyronine deiodinases, BW = body weight, DMEM = Dulbecco's Modified Eagle's Medium, DMSO = dimethyl sulfoxide, MTT = methyl thiazolyl tetrazolium, DTNB = dithio-bis-nitrobenzoic acid, GAPDH = glyceraldehyde phosphate dehydrogenase, SOD = superoxide dismutase, CAT = catalase, T-AOC = total antioxidant capacity, iNOS = inducible nitric oxide synthase, MAPK = mitogen-activated protein kinase, RT = reverse transcriptase, RT-PCR = real-time polymerase chain reaction, PBS = phosphate buffered solution

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Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1)

Cited by 39 publications

References 40 publications

Chemical–nutritional quality and oxidative stability of milk and dairy products obtained from Friesian cows fed with a dietary supplementation of dried grape pomace

Chemical–nutritional quality and oxidative stability of milk and dairy products obtained from Friesian cows fed with a dietary supplementation of dried grape pomace

Inflammatory and metabolic responses to an intramammary lipopolysaccharide challenge in early lactating cows supplemented with conjugated linoleic acid

Effects of retinoic acid on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells in vitro

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