Conjugated linoleic acid improves glucose utilization in the soleus muscle of rats fed linoleic acid–enriched and linoleic acid–deprived diets

Fariña, Ana Clara; Hirabara, Sandro Massao; Saín, Juliana; Latorre, María Emilia; González, Marcela Hebe; Curi, Rui; Bernal, Claudio

doi:10.1016/j.nutres.2014.09.008

Cited by 8 publications

(13 citation statements)

References 45 publications

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“…We cannot deny that some of these mechanisms could be involved in our experiment. Furthermore, in a previous work we studied glucose utilization in isolated skeletal muscle and demonstrated that CLA lowered the insulin response capacity for glucose utilization at both dietary LA levels, effect that could be mediated by leptin and adiponectin …”

Section: Discussionmentioning

confidence: 99%

Effect of Conjugated Linoleic Acids on Nutritional Status and Lipid Metabolism in Rats Fed Linoleic‐Acid‐Deprived Diets

Fariña

Lavandera

González

et al. 2019

Euro J Lipid Sci & Tech

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This study aims to investigate the effect of conjugated linoleic acid (CLA) on nutritional parameters and triacylglycerol (TAG) regulation in male Wistar rats fed linoleic acid (LA)‐deprived (−LA) diets compared to LA‐enriched (+LA) diets. In both +LA and −LA groups, CLA are incorporated into the tissues, showing higher levels in the adipose tissue. However, different metabolic and nutritional effects are observed depending on the LA status. CLA markedly reduces fat depots in the −LA group, associated with an increased lipoprotein lipase (LPL) and lipogenic enzyme activities as compensatory mechanisms. Moreover, CLA restores the hepatic TAG levels in −LA animals, associated with a normalized triacylglycerol‐secretion rate (TAG‐SR), an increased lipogenic enzyme activity and higher mRNA levels of fatty acid synthase. Serum TAG levels are not affected by CLA in the +LA group. However, in the −LA group, CLA decreases the TAG levels associated with a reduced TAG‐SR and a higher adipose tissue LPL activity. Thus, the CLA effects on the nutritional parameters and TAG metabolism differs depending on the LA status. CLA causes certain beneficial biological and nutritional effects in LA‐deprived but not in LA‐enriched animals. Practical Applications: The approach by the authors involve growing animals in healthy physiological conditions fed with diets containing recommended levels of dietary fats, moderate amounts of commercial CLA mixture obtained from industrial synthesis (equimolecular amounts of 9c,11t‐ and 10t,12c‐isomers), and unbalanced LA levels. These variables constitute a situation observed in the human population. The present study might contribute to understanding the role of CLA on nutritional parameters and TAG metabolism depending on the nutritional milieu. Conjugated linoleic acid effects on nutritional parameters and triacylglycerol metabolism in rats fed linoleic‐acid‐enriched and linoleic‐acid‐deprived diets are determined.

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Section: Discussionmentioning

confidence: 99%

Effect of Conjugated Linoleic Acids on Nutritional Status and Lipid Metabolism in Rats Fed Linoleic‐Acid‐Deprived Diets

Fariña

Lavandera

González

et al. 2019

Euro J Lipid Sci & Tech

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“…The composition of fatty acids in gastrocnemius muscle (100 mg) was determined by gas chromatography, according methods previously described (Feldsine et al 2002;Ratnayake et al 2002). A similar procedure has been used in our studies (Afonso et al 2013;Hirabara et al 2013;Fariña et al 2014;de Santana et al 2015). The results are presented as the mean ± SD of four animals per group.…”

Section: Determination Of Gastrocnemius Muscle Fatty Acid Compositionmentioning

confidence: 99%

Contractile function recovery in severely injured gastrocnemius muscle of rats treated with either oleic or linoleic acid

Abreu

Pinheiro

Vitzel

et al. 2016

Experimental Physiology

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What is the central question of this study? Oleic and linoleic acids modulate fibroblast proliferation and myogenic differentiation in vitro. However, their in vivo effects on muscle regeneration have not yet been examined. We investigated the effects of either oleic or linoleic acid on a well-established model of muscle regeneration after severe laceration. What is the main finding and its importance? We found that linoleic acid increases fibrous tissue deposition and impairs muscle regeneration and recovery of contractile function, whereas oleic acid has the opposite effects in severely injured gastrocnemius muscle, suggesting that linoleic acid has a harmful effect and oleic acid a potential therapeutic effect on muscle regeneration. Oleic and linoleic acids control fibroblast proliferation and myogenic differentiation in vitro; however, there was no study in skeletal muscle in vivo. The aim of this study was to evaluate the effects of either oleic or linoleic acid on the fibrous tissue content (collagen deposition) of muscle and recovery of contractile function in rat gastrocnemius muscle after being severely injured by laceration. Rats were supplemented with either oleic or linoleic acid for 4 weeks after laceration [0.44 g (kg body weight) day ]. Muscle injury led to an increase in oleic-to-stearic acid and palmitoleic-to-palmitic acid ratios, suggesting an increase in Δ desaturase activity. Increased fibrous tissue deposition and reduced isotonic and tetanic specific forces and resistance to fatigue were observed in the injured muscle. Supplementation with linoleic acid increased the content of eicosadienoic (20:2, n-6) and arachidonic (20:4, n-6) acids, reduced muscle mass and fibre cross-sectional areas, increased fibrous tissue deposition and further reduced the isotonic and tetanic specific forces and resistance to fatigue induced by laceration. Supplementation with oleic acid increased the content of docosahexaenoic acid (22:6, n-3) and abolished the increase in fibrous tissue area and the decrease in isotonic and tetanic specific forces and resistance to fatigue induced by muscle injury. We concluded that supplementation with linoleic acid impairs muscle regeneration and increases fibrous tissue deposition, resulting in impaired recovery of contractile function. Oleic acid supplementation reduced fibrous tissue deposition and improved recovery of contractile function, attenuating the tissue damage caused by muscle injury.

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“…The insulin used was Humulin R (U-100), which was acquired from Eli Lilly (Indianapolis, IN, USA). Amersham International (Bucks, UK) provided the 2-deoxy-[2,6-3 H]d-glucose and [U 14 C]d-glucose.…”

Section: Methodsmentioning

confidence: 99%

“…The detection limit for the main FAME identified ranged from 0.01 to 0.03%. Similar procedure was used in our previous studies [9,14,33].…”

Section: Fatty Acid Composition In Serum Muscle and Dietary Lipidsmentioning

confidence: 96%

“…Nevertheless, the dietary FA composition and the different TFA isomers present in the experimental diets and/or tissues have been shown to be the determining factors. The 11t-18:1, in opposition to other t-18:1 isomers, has a beneficial effect on glucose metabolism in rats probably through its bioconversion to 9c,11t-CLA [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Influence of trans fatty acids on glucose metabolism in soleus muscle of rats fed diets enriched in or deprived of linoleic acid

et al. 2017

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Dietary TFA enhanced glucose utilization in incubated soleus muscle under basal conditions and prevented the palmitate-induced inhibition in insulin-stimulated conditions. However, TFA reduced the insulin response to glucose uptake and metabolism. The effects mentioned above were influenced by the FA profile modifications induced by the dietary LA levels, suggesting that lipid metabolization and incorporation into plasma membrane are important determining factors of glucose metabolism and insulin sensitivity.

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Conjugated linoleic acid improves glucose utilization in the soleus muscle of rats fed linoleic acid–enriched and linoleic acid–deprived diets

Cited by 8 publications

References 45 publications

Effect of Conjugated Linoleic Acids on Nutritional Status and Lipid Metabolism in Rats Fed Linoleic‐Acid‐Deprived Diets

Effect of Conjugated Linoleic Acids on Nutritional Status and Lipid Metabolism in Rats Fed Linoleic‐Acid‐Deprived Diets

Contractile function recovery in severely injured gastrocnemius muscle of rats treated with either oleic or linoleic acid

Influence of trans fatty acids on glucose metabolism in soleus muscle of rats fed diets enriched in or deprived of linoleic acid

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