Conjugated and non-conjugated octadecaenoic acids affect differently intestinal acyl coenzyme A: Cholesterol acyltransferase activity

Lam, Cheuk Kai; Chen, Jingnan; Cao, Ying; Yang, Lin; Wong, Yin Mei; Yeung, Sai Ying Venus; Yao, Xiaoqiang; Huang, Yü; Chen, Zhenyu

doi:10.1016/j.atherosclerosis.2007.11.001

Cited by 35 publications

(39 citation statements)

References 28 publications

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“…Although, our first assumption for the role of L-arginine in the prevention of atherogenesis progress was based on the premise that arginine exerts its effect through facilitating increase of endothelial NO formation as reported by several investigators (Cooke et al, 1992;Brandes et al, 2000), however, our findings showed that supplementation of L-arginine at a concentration of 2% in drinking water did not significantly change the serum concentrations of TC and LDL-C in atherogenic rats. Lam et al, (2008) reported that unsaturated fatty acids inhibit the activity of intestinal acyl coenzyme A: cholesterol acyltransferase (ACAT), the rate-limiting step in cholesterol absorption, from this view, and as a rich source of poly unsaturated fatty acids, the effect of pumpkin seeds on the serum concentrations of TC in atherogenic rats could be explained .…”

Section: Discussionmentioning

confidence: 99%

The Effect of Pumpkin (<i>Cucurbita pepo</i> L) Seeds and L-Arginine Supplementation on Serum Lipid Concentrations in Atherogenic Rats

Abuelgassim

AL-showayman²

2011

Afr. J. Trad. Compl. Alt. Med.

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The present study aimed to examine the effect of pumpkin (Cucurbita pepo L.) seeds supplementation on androgenic diet-induced atherosclerosis. Rat were divided into two main groups , normal control and atherogenic control rats , each group composed of three subgroups one of them supplemented with 2% arginine in drinking water and the other supplemented with pumpkin seeds in diet at a concentration equivalent to 2% arginine. Supplementation continued for 37 days. Atherogenic rats supplemented with pumpkin seeds showed a significant decrease (p<0.001) in their serum concentrations of total cholesterol and LDL -C as they dropped from 4.89 mmol / L to 2.55 mmol /L and from 3.33 mmol / L to 0.70 mmol / L respectively. Serum concentrations of HDL-C were also significantly elevated in the same group. Although, atherogenic rats supplemented with 2% arginine showed significant increase in serum concentration of HDL-C, no significant changes were observed in their serum concentrations of total cholesterol and LDL-C. Our results showed that treatment of atherogenic rats with pumpkin seeds significantly decreased serum concentrations of TC and LDL-C. Our findings suggest that pumpkin seeds supplementation has a protective effect against atherogenic rats and this protective effect was not attributed to the high arginine concentrations in pumpkin seeds.

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Section: Discussionmentioning

confidence: 99%

The Effect of Pumpkin (<i>Cucurbita pepo</i> L) Seeds and L-Arginine Supplementation on Serum Lipid Concentrations in Atherogenic Rats

Abuelgassim

AL-showayman²

2011

Afr. J. Trad. Compl. Alt. Med.

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“…Protein concentration of two fractions was determined using a protein concentration assay kit obtained from BioRad (Hercules, CA, USA). LDL-R, CYP7A1, and HMG-CoA-R were quantified as we previously described [9]. The membrane protein was size-fractionated on a 7% SDS-PAGE gel.…”

Section: Western Blotting Analyses Of Liver Srebp-2mentioning

confidence: 99%

“…mRNA levels for liver SREBP-2, LDL-R, HMG-CoA-R, LXR-a, and CYP7A1 were quantified as previously described (9). In brief, total liver mRNA was extracted and isolated using Tizolm Reagent (Invitrogen, Carslbad, CA, USA).…”

Section: 5mentioning

confidence: 99%

Choosing hamsters but not rats as a model for studying plasma cholesterol‐lowering activity of functional foods

Zhang

Wang

Jiao

et al. 2009

Molecular Nutrition Food Res

Self Cite

105

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Rats and hamsters are commonly used rodents to test the efficacy of cholesterol-lowering functional foods. In general, a diet containing 1% cholesterol for rats whereas a diet containing 0.1% cholesterol for hamsters is used to induce the hypercholesterolemia. The present study was carried out to compare hamsters with rats as a hypercholesterolemia model. Golden Syrian hamsters and Sprague Dawley rats were randomly divided into four groups and fed one of the four diets containing 0-0.9% cholesterol. Results demonstrated that serum total cholesterol (TC) in hamsters was raised 73-81% higher than that in rats fed the same cholesterol diets. Unlike rats in which HDL-C accounted very little for serum TC, the lipoprotein profile in hamsters was closer to that in humans. We investigated interaction of higher cholesterol diets with 3-hydroxy-3-methylglutary-CoA (HMG-CoA) reductase, low-density lipoprotein receptor (LDL-R) and cholesterol-7alpha-hydroxylase (CYP7A1), sterol regulatory element binding protein-2 (SREBP-2), and liver X receptor (LXR-alpha). Results showed hamsters and rats metabolized cholesterol differently. In view that hamsters synthesize and excrete cholesterol and bile acids in a manner similar to that in humans, it is concluded that hamsters but not rats shall be chosen as a model to study efficacy of cholesterol-lowering functional foods.

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“…Information concerning the biological activity of CLNAs is scarce, except for some studies which have demonstrated that CLNAs have a cytotoxic effect on cultured human tumor cells (IGARASHI; MIYAZAWA, 2005;YASUI et al, 2005), inhibits carcinogenesis (KOHNO et al, 2004a, b;TSUZUKI et al, 2004a;TSUZUKI;KAWAKAMI, 2008), and alters lipid metabolism in animals (YANG et al, 2005;YAMASAKI et al, 2006;LAM et al, 2008). Some mechanism of the antitumor effects of CLNAs is thought to be the apoptosis in mouse liver.…”

Section: Health Effectsmentioning

confidence: 99%

“…In contrast, the weights of the perirenal and epididymal adipose tissues were significantly decreased while the liver TG level and weight was significantly increased in mice fed a diet supplemented with CLAs (YUAN et al, 2009e). Lam et al (2008) examined the relative hypocholesterolemic activity of CLAs, CLNAs, and their corresponding nonconjugated, LA and LNA. They found that LA, CLAs and LNA were hypocholesterolemic, while CLNAs had no effect on blood cholesterol level in hamsters fed a high cholesterol diet.…”

Section: Clas Versus Clnasmentioning

confidence: 99%

Chemical and physiological aspects of isomers of conjugated fatty acids

Carvalho¹,

Melo²,

Mancini‐Filho³

2010

Ciênc. Tecnol. Aliment.

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Conjugated and non-conjugated octadecaenoic acids affect differently intestinal acyl coenzyme A: Cholesterol acyltransferase activity

Cited by 35 publications

References 28 publications

The Effect of Pumpkin (<i>Cucurbita pepo</i> L) Seeds and L-Arginine Supplementation on Serum Lipid Concentrations in Atherogenic Rats

The Effect of Pumpkin (<i>Cucurbita pepo</i> L) Seeds and L-Arginine Supplementation on Serum Lipid Concentrations in Atherogenic Rats

Choosing hamsters but not rats as a model for studying plasma cholesterol‐lowering activity of functional foods

Chemical and physiological aspects of isomers of conjugated fatty acids

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