Conjugal Transfer of Broad-Host-Range Plasmid pAMβ1 into Enteric Species of Lactic Acid Bacteria

Vescovo, M.; Morelli, L.; Bottazzi, V.; Gasson, M. J.

doi:10.1128/aem.46.3.753-755.1983

Cited by 78 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extrachromosomal DNA was extracted for plasmid screening by alkaline lysis according to Vescovo et al [12] and separated by agarose gel electrophoresis (0.6^0.8% agarose) in 1UTAE bu¡er. Plasmid sizes were determined by comparing the bands with the Supercoiled DNA molecular weight marker (Gibco-BRL).…”

Section: Plasmid Analysismentioning

confidence: 99%

Strain typing among enterococci isolated from home-made Pecorino Sardo cheese

Mannu

1999

FEMS Microbiology Letters

View full text Add to dashboard Cite

Three molecular techniques (RAPD-polymerase chain reaction analysis, plasmid profile and pulsed-field gel electrophoresis) were used for a preliminary approach to type, at strain level, enterococci isolated from a 24-h-old home-made Pecorino Sardo (protected designation of origin) cheese. A high genetic polymorphism was found. Clusters obtained by the RAPD technique and plasmid profile analysis contained different strains. Pulsed-field gel electrophoresis proved to be an efficient and highly reproducible typing method. In addition, by combining the results from plasmid profile analysis and pulsed-field gel electrophoresis, it was possible to identify closely related strains probably belonging to the same clonal lineage. z 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

show abstract

Section: Plasmid Analysismentioning

confidence: 99%

Strain typing among enterococci isolated from home-made Pecorino Sardo cheese

Mannu

1999

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…The introduction of broad host range transmissible plasmids was one of the earliest approaches used to establish basic genetic systems in the lactic acid bacteria. There are many reports of the conjugal transfer of the enterocoocal plasmid pAM/H into different strains, species and genera [64][65][66][67][68][69][70] although in some cases better results were obtained when alternative plasmids were used as in the transfer of piP501 in Pediococcus [71]. In matings between L, iactis and Bacillus subtilis a specific 10.5-kb deletion of pAMOl was observed and this prevented subsequent conjugal transfer [72].…”

Section: Heterologous Broad Host Range Transmissible Plasmidsmentioning

confidence: 99%

In vivo genetic systems in lactic acid bacteria

Gasson

1990

FEMS Microbiology Letters

View full text Add to dashboard Cite

SUMMARYA review of in vivo genetic systems covers the key features of transduction and conjugation but emphasises the intramolecular and intermolecular DNA interactions that are often associated with these processes. As well as the transfer of many lactose plasmids, conjugal transfer of nisin genes and the use of conjugation to construct bacteriophage-resistant dairy starter cultures are discussed. The discovery and characterization of insertion sequences in Lactobacillus and Lactococcus and the exploitation of heterologous conjugation and transposition systems in the lactic acid bacteria are described.

show abstract

“…There is evidence that the broad host range plasmids PAM81 and/or pIP501 can undergo conjugal transfer from streptococci into enteric lactobacilli (Vescovo et al 1983), L p t . casei (Gibson et al 1979), Pediococcus spd(Gonza1ez & Kunka 1983) and Lact.…”

mentioning

confidence: 99%

Evidence for the conjugal transfer of the broad host range plasmid pIP501 into strains of Lactobacillus helveticus

Thompson¹,

Collins

1988

Journal of Applied Bacteriology

View full text Add to dashboard Cite

The conjugative broad host range plasmid pIP501 was transferred from Streptococcus faecalis to a series of strains of lactic streptococci used commercially as dairy starter cultures. With these transconjugants as donors the plasmid was exconjugated to two strains of Lactobacillus helveticus and a commercially used strain of Strep. thermophilus. There was evidence that the plasmid could transfer between isogenic derivatives of one of the strains of Lact. helveticus. Transfer from Lact. helveticus to Strep. faecalis was also detected but at a low frequency. There was no evidence for the conjugal transfer of plasmid pIP501 into a strain of Lact. bulgaricus by exconjugation from either lactic streptococci or Lactobacillus sp.

show abstract

Conjugal Transfer of Broad-Host-Range Plasmid pAMβ1 into Enteric Species of Lactic Acid Bacteria

Cited by 78 publications

References 15 publications

Strain typing among enterococci isolated from home-made Pecorino Sardo cheese

Strain typing among enterococci isolated from home-made Pecorino Sardo cheese

In vivo genetic systems in lactic acid bacteria

Evidence for the conjugal transfer of the broad host range plasmid pIP501 into strains of Lactobacillus helveticus

Contact Info

Product

Resources

About