Coniferyl and sinapyl alcohols: major phenylpropanoids released in hot water extracts of tobacco and alfalfa

Characterization of cDNAs associated with lignification and their expression profiles in loquat fruit with different lignin accumulation

“…Extraction and HPLC analysis of free coniferyl and sinapyl alcohols in the Xesh tissues were performed using modiWcations of the method of Andersen et al (1980). Three grams of tissue was ground and extracted twice, with 30 ml and then 10 ml of ethyl acetate.…”

Characterization of Poplar Lignins Acidolysis Products: Capillary Gas-Liquid and Liquid-Liquid Chromatography of Monomeric Compounds

“…On the contrary, their presence in acid solvolytic medium seemed to be quite unlikely, owing to their high tendency to condensation reaction, (Freudenberg et al 1964). However sinapyl alcohol, also observed in the alkaline hydrolysate of poplar wood (unpublished results), could be identified after derivatization, mainly äs TMS trans isomer (peak 26) and with reference to literature data (Andersen et al 1980) and authentic TMS compound; it could be seen that TMS coniferyl alcohol had same retention time than TMS ß-oxyconiferyl alcohol (ketonic form, peak 20); the resolution of the corresponding peak by Biemann analysis could not be performed, owing to the high number of common fragments and close overlapping. Thus, coniferyl alcohol could not be characterized without ambiguity, although it was very likely present.…”

Effect of plant growth and air curing on amounts of trans-coniferyl and trans-sinapyl alcohols in midveins of burley tobacco

“…p-Coumaric acid, ferulic acid, sinapic acid, and caffeic acid were obtained from Aldrich Chemical Co., Milwaukee, WI. p-Coumaraldehyde, coniferaldehyde, sinapaldehyde, and their alcohol congeners were synthesized by reduction of their acetylated acid chlorides with metal hydrides (Andersen et al, 1980). Caffeic acid n-propyl ester was used as the internal standard for most GC analyses and was prepared by the reaction of caffeic acid and n-propyl alcohol saturated with HC1 gas (Andersen and Vaughn, 1970).…”

“…Coniferyl alcohol [3-(4-hydroxy-3-methoxyphenyl)-2propen-l-ol] and sinapyl alcohol [3-(-hydroxy-3,5-dimethoxyphenyl)-2-propen-l-ol] are considered to be precursors of the monolignol building blocks of plant lignin (Freudenberg, 1966), and they were recently identified as phenolic phenylpropanoids in hot-water extracts of hurley tobacco stalk (Andersen et al, 1980). These alcohols were thought to be weakly bound to non-cross-lined regions of Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, and Department of Agronomy, University of Kentucky, Lexington, Kentucky 40546. lignin or polysaccharide and capable of release by hydrolysis with hot water.…”