Conifer somatic embryogenesis for studies of plant cell biology

Fowke, Larry C.; Attree, S. M.; Binarová, Pavla; Galway, Moira E.; Wang, H.

doi:10.1007/bf02632218

Cited by 7 publications

(4 citation statements)

References 44 publications

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“…Somatic embryo production and cryopreservation from embryogenic cells of several important species have been conducted (Sutton, 2002). Furthermore, SE is an ideal system for synthetic seed technology (Reddy et al, 2012), genetic engineering (Henderson and Walter, 2006), and plant cell biology studies (Fowke et al, 1995). Therefore, numerous studies on SE concentrate on the development and improvement of methods for mass propagation in conifers (Tautorus et al, 1991; Jain et al, 1995b; Klimaszewska and Cyr, 2002; Stasolla et al, 2002; Jain and Gupta, 2005; Klimaszewska et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines (Pinus thunbergii, Pinus densiflora, Pinus armandii var. amamiana, and Pinus luchuensis). Induction of embryogenic tissues (ET), embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in Pinus species through to SE.

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Section: Introductionmentioning

confidence: 99%

Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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“…Somatic embryogenesis (SE) has been reported for many commercially important gymnosperms (Fowke et al 1993;Park 2002;Sutton 2002;Tautorus et al 1991). SE proceeds through a sequence of steps in vitro including initiation, multiplication, maturation, and germination.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in loblolly pine ( Pinus taeda L.): improving culture initiation with abscisic acid and silver nitrate

Pullman¹,

Namjoshi²,

Zhang³

2003

Plant Cell Reports

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Loblolly pine ( Pinus taeda L.) culture initiation was improved by the addition of abscisic acid (ABA) (3.7 micro M), silver nitrate (20 micro M), and guanosine 3',5'-cyclic monophosphate, 8-bromo-, sodium salt (10 micro M) to the medium and by raising cytokinin levels in the presence of 50 mg/l activated carbon (AC). Basal medium contained modified 1/2-P6 salts, 50 mg/l AC, Cu and Zn added to compensate for adsorption by AC, 1.5% maltose, 2% myo-inositol, 500 mg/l casamino acids, 450 mg/l glutamine, 2 mg/l alpha-naphthaleneacetic acid (NAA), 0.55 mg/l 6-benzylaminopurine (BA), 0.53 mg/l kinetin, and 2 g/l Gelrite. Across 32 open-pollinated families initiation ranged from 0 to 53.4%, with an average of 17.9%. Further optimization of cytokinins to 0.63 mg/l BA and 0.61 mg/l kinetin along with the removal of ABA maintained initiation at 18.2% across 19 families. Survival of 2001 new initiations was tracked for 4-6 months. Survival averaged 28.8%. A test of 68 new initiations tracked closely for 4 months demonstrated that at least 80% of the cultures lost did not grow after transfer to the multiplication media, suggesting that many new initiations abort during the initiation process.

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“…Conifer somatic embryogenesis (SE) has been demonstrated for many genera [7,14,20,21]. SE proceeds through initiation, multiplication, maturation, and germination.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in loblolly pine (Pinus taeda L.): improving culture initiation rates

Pullman¹,

Johnson²

2002

Ann. For. Sci.

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-Loblolly pine (Pinus taeda L.) is one of the most important commercial trees in the U.S. To be successful for commercial use, somatic embryogenesis technology must work with a variety of genetically diverse seeds. Initiation rates of loblolly pine were improved through a combination of modified 1/2 P6 salts, activated carbon at 50-100 mgL -1 , Cu and Zn added to compensate for adsorption by activated carbon, 1.5% maltose, 2% myo-inositol, (to raise osmotic level partially simulating the ovule environment), 500 mg L -1 case amino acids, 450 mg L -1 glutamine, 2 mg L -1 NAA, 0.45 mg L -1 BAP, 0.43 mg L -1 kinetin, and 1.6-2 g L -1 Gelrite. Across 10 open-pollinated families, initiation rates ranged from 3-33%, averaging 16%. Loblolly pine / somatic embryogenesis / initiation / conifer / Pinus taeda Résumé

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Conifer somatic embryogenesis for studies of plant cell biology

Cited by 7 publications

References 44 publications

Progress in Somatic Embryogenesis of Japanese Pines

Progress in Somatic Embryogenesis of Japanese Pines

Somatic embryogenesis in loblolly pine ( Pinus taeda L.): improving culture initiation with abscisic acid and silver nitrate

Somatic embryogenesis in loblolly pine (Pinus taeda L.): improving culture initiation rates

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