Conidiation in Neurospora crassa

Turian, G.; Bianchi, D. E.

doi:10.1007/bf00408119

Cited by 24 publications

(10 citation statements)

References 20 publications

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“…We also report the identification and initial characterization of the developmental regulator, csp-1. Asexual development in Neurospora and other filamentous fungi is a complex and incompletely understood process, integrating signals from the circadian clock, metabolic state, and various types of external and internal stress (Turian and Bianchi 1971). This developmental process involves multiple intracellular signaling events including MAPK (Pandey et al 2004), cAMP (Banno et al 2005), and Ras-dependent pathways (Belden et al 2007).…”

Section: Discussionmentioning

confidence: 99%

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

Lambreghts¹,

Shi²,

Belden³

et al. 2009

Genetics

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We report the discovery and validation of a set of single nucleotide polymorphisms (SNPs) between the reference Neurospora crassa strain Oak Ridge and the Mauriceville strain (FGSC 2555), of sufficient density to allow fine mapping of most loci. Sequencing of Mauriceville cDNAs and alignment to the completed genomic sequence of the Oak Ridge strain identified 19,087 putative SNPs. Of these, a subset was validated by cleaved amplified polymorphic sequence (CAPS), a simple and robust PCR-based assay that reliably distinguishes between SNP alleles. Experimental confirmation resulted in the development of 250 CAPS markers distributed evenly over the genome. To demonstrate the applicability of this map, we used bulked segregant analysis followed by interval mapping to locate the csp-1 mutation to a narrow region on LGI. Subsequently, we refined mapping resolution to 74 kbp by developing additional markers, resequenced the candidate gene, NCU02713.3, in the mutant background, and phenocopied the mutation by gene replacement in the WT strain. Together, these techniques demonstrate a generally applicable and straightforward approach for the isolation of novel genes from existing mutants. Data on both putative and validated SNPs are deposited in a customized public database at the Broad Institute, which encourages augmentation by community users.

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Section: Discussionmentioning

confidence: 99%

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

Lambreghts¹,

Shi²,

Belden³

et al. 2009

Genetics

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“…Yoshida and K. Hasunuma, unpublished data). Furthermore, notably, sources of carbon such as glucose aVect hyphal growth and conidial formation (Turian and Bianchi 1971;Ebbole 1998). The sod-1 and bd mutants have no characteristics of excess-conidiation, although bd showed excess conidiation under nutrient-rich conditions (Supplemental Table 2).…”

Section: Roles Of Ros and Sod-1 On Conidiation Rhythmmentioning

confidence: 99%

Conidiation rhythm and light entrainment in superoxide dismutase mutant in Neurospora crassa

et al. 2007

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Conidial formation in the filamentous fungus Neurospora crassa is regulated by nutritional conditions, light, and the circadian clock. We found that a sod-1 mutant, with a defective superoxide dismutase catalyzing the conversion of superoxide to hydrogen peroxide, had a slightly shorter period length than the wild type and clear conidial banding similar to a mutant of band (bd). However, unlike the bd mutant, the sod-1 mutant could sustain conidial banding with light pulses on a nutrient-rich medium, which involved an enhancement of the light-induced transcription of frequency (frq). sod-1 was hypersensitive to entrainment of the conidiation rhythm by light in race tubes. Furthermore, a frq(10); sod-1 double mutant showed conidiation rhythm in darkness and could be synchronized to light/dark cycles by the masking effect of light. These genetic analyses suggested that intracellular reactive oxygen species (ROS) act on circadian conidiation via multiple circadian clocks and output pathways.

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“…Formation of macroconidia, known as macroconidiation or conidiation (as it will be referred to throughout this paper), has been extensively studied at the morphological level (32,40,53,59,64). Upon receipt of certain environmental cues, e.g., carbon starvation, vegetative tissue, the mycelium, initiates conidiation by producing aerial hyphae that grow away from the nutrient surface.…”

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confidence: 99%

Characterization of rco-1 of Neurospora crassa, a Pleiotropic Gene Affecting Growth and Development That Encodes a Homolog of Tup1 of Saccharomyces cerevisiae

Yamashiro

Ebbole

Lee

et al. 1996

Molecular and Cellular Biology

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The filamentous fungus Neurospora crassa undergoes a well-defined developmental program, conidiation, that culminates in the production of numerous asexual spores, conidia. Several cloned genes, including con-10, are expressed during conidiation but not during mycelial growth. Using a previously described selection strategy, we isolated mutants that express con-10 during mycelial growth. Selection was based on expression of an integrated DNA fragment containing the con-10 promoter-regulatory region followed by the initial segment of the con-10 open reading frame fused in frame with the bacterial hygromycin B phosphotransferase structural gene (con10-hph). Resistance to hygromycin results from mutational alterations that allow mycelial expression of the con-10-hph gene fusion. A set of drug-resistant mutants were isolated; several of these had abnormal conidiation phenotypes and were trans-acting, i.e., they allowed mycelial expression of the endogenous con-10 gene. Four of these had alterations at a single locus, designated rco-1 (regulation of conidiation). Strains with the rco-1 mutant alleles were aconidial, female sterile, had reduced growth rates, and formed hyphae that coiled in a counterclockwise direction, opposite that of the wild type. The four rco-1 mutants had distinct conidiation morphologies, suggesting that conidiation was blocked at different stages. Wild-type rco-1 was cloned by a novel procedure employing heterokaryon-assisted transformation and ligation-mediated PCR. The predicted RCO1 polypeptide is a homolog of Tup1 of Saccharomyces cerevisiae, a multidomain protein that mediates transcriptional repression of genes concerned with a variety of processes. Like tup1 mutants, null mutants of rco-1 are viable and pleiotropic. A promoter element was identified that could be responsible for RCO1-mediated vegetative repression of con-10 and other conidiation genes.Neurospora crassa, a filamentous fungus, has three distinct sporulation pathways that lead to the production of either of two types of asexual spores, macroconidia and microconidia, or sexual spores, ascospores. Formation of macroconidia, known as macroconidiation or conidiation (as it will be referred to throughout this paper), has been extensively studied at the morphological level (32,40,53,59,64). Upon receipt of certain environmental cues, e.g., carbon starvation, vegetative tissue, the mycelium, initiates conidiation by producing aerial hyphae that grow away from the nutrient surface. These hyphae undergo apical budding, generating what have been termed minor constriction chains. Subsequent growth by continued apical budding leads to hyphal segments with more pronounced, major constrictions. These proconidial chains continue to differentiate by completing septation at the sites of major constrictions. Just prior to disarticulation of mature conidia, a fragile structure, termed the connective (59), is evident. The connective joins adjacent proconidia and is ultimately cleaved. The entire process of conidiation takes 12 to 24 h. Microc...

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Conidiation in Neurospora crassa

Cited by 24 publications

References 20 publications

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

Conidiation rhythm and light entrainment in superoxide dismutase mutant in Neurospora crassa

Characterization of rco-1 of Neurospora crassa, a Pleiotropic Gene Affecting Growth and Development That Encodes a Homolog of Tup1 of Saccharomyces cerevisiae

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