Conidial alkaline phosphatase from Neurospora crassa

THE IDENTIFICATION AND PURIFICATION OF A CELL‐SURFACE ALKALINE PHOSPHATASE FROM THE DINOFLAGELLATE PROROCENTRUM MINIMUM (DINOPHYCEAE)1

“…Alkaline phosphatases purified from most microorganisms range from a denatured molecular weight of about 35,000 daltons (Say et al 1996) to about 145,000 daltons (Ray et al 1991). However, alkaline phosphatases isolated from multicellular organisms are larger.…”

Ectoenzymes in Prorocentrum minimum

“…This indicates that the protein is multimeric, as is the case for alkaline phosphatase in Escherichia coli (Coleman, 1992). The P. minimum AP is large relative to that of other microorganisms which generally range from a denatured molecular weight of about 35,000 Da in Neurospora crassa (Say et al, 1996) to about 145,000 Da in Synechococcus (Ray et al, 1991).…”

Effect of carbon source on alkaline phosphatase production and excretion in Aspergillus caespitosus

“…As a negative control, the chelating agent, 1 mM EDTA, was added to the incubation medium, since phosphatase activity is dependent on magnesium and others metallic ions (SAY et al 1996). The presence of alkaline phosphatase in the mycelia was confirmed in both cases (Fig.…”

“…Although thermophilic fungi are considered as good sources of thermostable enzymes, their phosphatases are poorly characterized. Some alkaline phosphatases from thermophilic fungi have recently been purified and characterised in our laboratory (SAY et al 1996, BUAINAIN et al 1998, GUIMARÃES et al 2001. In the present study we describe the production of extracellular thermostable alkaline phosphatases from a thermotolerant fungus Aspergillus caespitosus.…”