Conical diffraction as a versatile building block to implement new imaging modalities for superresolution in fluorescence microscopy

Fallet, Clément; Colomb, Julien; Oddos, Stéphane; Tinévez, Jean-Yves; Moisan, Lionel; Sirat, Gabriel Y.; Braitbart, Philippe O.; Shorte, Spencer

doi:10.1117/12.2061059

Cited by 7 publications

(9 citation statements)

References 10 publications

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“…Super‐resolution fluorescence imaging was performed with Conical Diffraction Microscopy (CODIM; Caron et al , ; Fallet et al , ). The BioAxial super‐resolution module (CODIM100, BioAxial) is an add‐on integrated to the Nikon confocal system previously described.…”

Section: Methodsmentioning

confidence: 99%

The Wnt/Ca ²⁺ pathway is involved in interneuronal communication mediated by tunneling nanotubes

Vargas

Loría

et al. 2019

The EMBO Journal

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Tunneling nanotubes (TNTs) are actin‐based transient tubular connections that allow direct communication between distant cells. TNTs play an important role in several physiological (development, immunity, and tissue regeneration) and pathological (cancer, neurodegeneration, and pathogens transmission) processes. Here, we report that the Wnt/Ca2+ pathway, an intracellular cascade that is involved in actin cytoskeleton remodeling, has a role in TNT formation and TNT‐mediated transfer of cargoes. Specifically, we found that Ca2+/calmodulin‐dependent protein kinase II (CaMKII), a transducer of the Wnt/Ca2+ pathway, regulates TNTs in a neuronal cell line and in primary neurons. We identified the β isoform of CaMKII as a key molecule in modulating TNT formation and transfer, showing that this depends on the actin‐binding activity of the protein. Finally, we found that the transfer of vesicles and aggregated α‐synuclein between primary neurons can be regulated by the activation of the Wnt/Ca2+ pathway. Our findings suggest that Wnt/Ca2+ pathway could be a novel promising target for therapies designed to impair TNT‐mediated propagation of pathogens.

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Section: Methodsmentioning

confidence: 99%

The Wnt/Ca ²⁺ pathway is involved in interneuronal communication mediated by tunneling nanotubes

Vargas

Loría

et al. 2019

The EMBO Journal

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“…Super-resolution imaging was performed with a commercial CoDiM ( Caron et al, 2014 ; Fallet et al, 2014 ). The BioAxial super-resolution module (CODIM100, BioAxial) is an add-on integrated to the Nikon confocal system previously described.…”

Section: Methodsmentioning

confidence: 99%

Optogenetic Light Sensors in Human Retinal Organoids

et al. 2018

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Optogenetic technologies paved the way to dissect complex neural circuits and monitor neural activity using light in animals. In retinal disease, optogenetics has been used as a therapeutic modality to reanimate the retina after the loss of photoreceptor outer segments. However, it is not clear today which ones of the great diversity of microbial opsins are best suited for therapeutic applications in human retinas as cell lines, primary cell cultures and animal models do not predict expression patterns of microbial opsins in human retinal cells. Therefore, we sought to generate retinal organoids derived from human induced pluripotent stem cells (hiPSCs) as a screening tool to explore the membrane trafficking efficacy of some recently described microbial opsins. We tested both depolarizing and hyperpolarizing microbial opsins including CatCh, ChrimsonR, ReaChR, eNpHR 3.0, and Jaws. The membrane localization of eNpHR 3.0, ReaChR, and Jaws was the highest, likely due to their additional endoplasmic reticulum (ER) release and membrane trafficking signals. In the case of opsins that were not engineered to improve trafficking efficiency in mammalian cells such as CatCh and ChrimsonR, membrane localization was less efficient. Protein accumulation in organelles such as ER and Golgi was observed at high doses with CatCh and ER retention lead to an unfolded protein response. Also, cytoplasmic localization was observed at high doses of ChrimsonR. Our results collectively suggest that retinal organoids derived from hiPSCs can be used to predict the subcellular fate of optogenetic proteins in a human retinal context. Such organoids are also versatile tools to validate other gene therapy products and drug molecules.

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“…In this context, CR has been presented as a flexible and cheap alternative for SR imaging. Based on a BC and polarizing optics a simple add‐on for a commercial laser‐scanning confocal microscope using the CR phenomenon has been designed . Before entering into the confocal microscope, the light beam is transformed by means of CR into a doughnut‐like light beam obtained for

ρ_{0} \approx 1

, as indicated in Fig.…”

Section: Technological Applications Of Conical Refractionmentioning

confidence: 99%

Conical refraction: fundamentals and applications

Turpin

Loiko

Kalkandjiev

et al. 2016

Laser & Photonics Reviews

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A mis padres, hermanos, sobrinos y a Raquel AcknowledgmentsTot té un principi i un final i aquest serà el punt final a una etapa de la meva vida la qual estic segur que d'aquí uns anys, quan miri enrere, veuré que ha estat probablement la que ha tingut una major rellevància tant a nivell personal com científic. Quan vaig acabar la carrera no tenia gaire clar quin camí seguir però vaig tenir la sort de parlar amb qui ha estat el meu director de tesi, el Jordi, qui em va guiar i va posar tota la seva confiança en mi des del primer moment. Em va oferir un tema original i es va aventurar a dirigir una tesi amb una forta vessant experimental, tot un atreviment venint d'un teòric! Quan vaig començar el Màster, si m'haguessin dit on he arribat 5 anys després mai m'ho hauria cregut i, Jordi, tu tens gran part de culpa de tot això. Moltes gràcies per haver confiat en mi des del primer dia, per haver-te mostrat sempre tan orgullós de tot el que he fet, per haver estat sempre disponible i per ajudar-me i animar-me quan he passat moments durs durant aquest anys. M'has ensenyat a ser un bon científic, a moure'm dins aquest món, a creure em mi i a ser una millor persona. Mai t'estaré prou agraït per tot això i per haver sigut tan proper amb els teus cotilleos, converses de futbol, política, noies, etc. dinant al bar. Has estat com un pare per a mi (bueno, un germà gran, que tampoc ets tan vell per a ser Mompare :P). Si el Jordi ha estat el meu pare en la ciència, el Yury ha estat el meu gurú científic. Mai he trobat una persona tan pacient, que sàpiga tant de qualsevol tema i amb tanta dedicació com tu. M'has ensenyat a ser riguròs, a tenir un mètode, la importància de estar al dia de tot el que es fa en el cap d'estudi, a programar, i un llarg etcètera. En resum, m'has ajudat a donar-me forma a mi mateix com a científic i a tenir perseverança en el que faig. Tot això sense oblidar que per a mi ets un gran amic! Seguidament, també he donar les gràcies al Todor, una de les persones més enèrgiques que conec i amb més passió per la ciència. Tu vas iniciar tot el tema de la refracció cònica al nostre grup i has demostrat que ets realment qui més coneix de cristalls i del fenomen en si. Si no hagués estat per tu, no podríem haver començat res d'això i no podríem haver explotat el fenomen ni una quarta part del que hem fet. Ets un exemple per tenir sempre un somriure a la cara i per aquesta iii iv força que et fa tirar endavant tots els teus projectes. També vull donar les gràcies a altres companys del Grup d'Òptica, començant per la Verònica, que va passar de ser una "professora de la carrera" a ser el meu ideal de constància i l'esforç. Ets un exemple professional a més a més d'una persona molt més propera i alegra del que pot semblar a primera vista. A Ramón también le tengo que agradecer su ayuda y su disponibilidad a lo largo de estos años, su paciencia cuando ha tenido que soportar mis imitaciones y su alegría y naturalidad en todo momento. Amb el Francesc també he compartit molts bons moments, sobretot a les diverses activit...

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Conical diffraction as a versatile building block to implement new imaging modalities for superresolution in fluorescence microscopy

Cited by 7 publications

References 10 publications

The Wnt/Ca ²⁺ pathway is involved in interneuronal communication mediated by tunneling nanotubes

The Wnt/Ca ²⁺ pathway is involved in interneuronal communication mediated by tunneling nanotubes

Optogenetic Light Sensors in Human Retinal Organoids

Conical refraction: fundamentals and applications

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Conical diffraction as a versatile building block to implement new imaging modalities for superresolution in fluorescence microscopy

Cited by 7 publications

References 10 publications

The Wnt/Ca 2+ pathway is involved in interneuronal communication mediated by tunneling nanotubes

The Wnt/Ca 2+ pathway is involved in interneuronal communication mediated by tunneling nanotubes

Optogenetic Light Sensors in Human Retinal Organoids

Conical refraction: fundamentals and applications

Contact Info

Product

Resources

About

The Wnt/Ca ²⁺ pathway is involved in interneuronal communication mediated by tunneling nanotubes

The Wnt/Ca ²⁺ pathway is involved in interneuronal communication mediated by tunneling nanotubes