Congo Red-Agar Plating Medium for Detecting Pigmentation in
            <i>Pasteurella pestis</i>

Surgalla, Michael J.; Beesley, Earl D.

doi:10.1128/am.18.5.834-837.1969

Cited by 121 publications

(19 citation statements)

References 5 publications

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“…During routine lab culture, Y. pestis incurs spontaneous loss of a 102-kb locus, referred to as the pigmentation locus ( 56 ), or Pgm locus, named for the ability to form pigmented colonies on CR-supplemented agar ( 57 ). The hmsHFRS operon is located within the pgm locus and confers this phenotype.…”

Section: Resultsmentioning

confidence: 99%

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CsrA Enhances Cyclic-di-GMP Biosynthesis and Yersinia pestis Biofilm Blockage of the Flea Foregut by Alleviating Hfq-Dependent Repression of the hmsT mRNA

Silva-Rohwer

Held

Sagawa

et al. 2021

mBio

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Yersinia pestis , the bacterial agent of bubonic plague, produces a c-di-GMP-dependent biofilm-mediated blockage of the flea vector foregut to facilitate its transmission by flea bite. However, the intricate molecular regulatory processes that underlie c-di-GMP-dependent biofilm formation and, thus, biofilm-mediated blockage in response to the nutritional environment of the flea are largely undefined.

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Section: Resultsmentioning

confidence: 99%

“…Bacterial strains and plasmids used in this study are listed in Table S1 in the supplemental material. The Y. pestis KIM6+ (pCD1 − ) strains were cultured on Congo red-heart infusion agar ( 57 ) to confirm the presence of the hmsHFRS locus prior to subsequent culturing. Strains were grown at 25°C with shaking unless otherwise stated.…”

Section: Methodsmentioning

confidence: 99%

CsrA Enhances Cyclic-di-GMP Biosynthesis and Yersinia pestis Biofilm Blockage of the Flea Foregut by Alleviating Hfq-Dependent Repression of the hmsT mRNA

Silva-Rohwer

Held

Sagawa

et al. 2021

mBio

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“…Previous studies have shown that the ability to bind to hemin in certain pathogenic bacteria is strongly associated with their ability to bind Congo red [14][15][16]. In a study of oral spirochetes (Treponema denticola, Treponema vincentii, and Treponema socranskii), the hemin binding property of bacteria was reported to be associated with Congo red binding [15].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the hemin-binding property of Porphyromonas endodontalis

Kim

Lee

2021

Oral Biol Res

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Porphyromonas endodontalis, found in the root canal of teeth, requires iron for growth. However, the mechanism of iron uptake in P. endodontalis remains unclear. The ability of bacteria to utilize heme compounds to acquire iron for growth has been reported in some pathogenic bacteria. In the present study, we analyzed the ability of P. endodontalis to obtain iron from heme compounds. Further, we investigated the hemin-binding characteristics of P. endodontalis and the relationship between hemin binding and Congo red binding. To confirm the bacterial growth in hemin-supplemented medium, iron was removed from the medium with an ironchelator, and hemin was supplemented to an iron-free medium. The hemin-binding characteristics of P. endodontalis were analyzed by incubating bacteria with hemin and measuring the optical density of the supernatant obtained via centrifugation, using hemin concentration standard curve. Although growth of P. endodontalis was not observed in the iron-depleted medium, it was observed in a hemin-supplemented medium. Further, hemin binding was dependent on the concentrations of hemin and bacteria. Hemin binding proceeded quickly in P. endodontalis, and the incubation temperature had no effect on this binding. Similar to hemin binding, Congo red binding of P. endodontalis was dependent on Congo red and bacterial concentrations. In addition, Congo red binding of P. endodontalis was inhibited by hemin prebinding. Hemin-agarose beads and SDS-PAGE were used to identify a 40-kDa protein that could be involved in hemin binding. The results showed that P. endodontalis could bind to and use hemin to obtain the iron required for growth.

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“…38103. common sugar in indicator cells of other species. However, sensitive cells of Y. pestis, in addition to being Pst-, are also able to absorb certain exogenous pigments (pgm+) such as hemin (14) or Congo red (28). The mutation to pgm-in Pst-cells of Y. pestis results in resistance to pesticin (5).…”

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confidence: 99%

Specificity, Induction, and Absorption of Pesticin

Yang

Brubaker

1972

J Bacteriol

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Irradiation of pesticinogenic (Pst + ) cells of Yersinia pestis with ultraviolet light resulted in a five- to eightfold increase in titer of intracellular pesticin. Extracellular activity in irradiated or control cultures never approached that found within the bacteria. Upon chromatography of crude extracts of Pst + cells on columns of diethylaminoethyl-cellulose, Sephadex G200, and calcium hydroxyapatite, fractions were recovered which inhibited the growth of indicator cells of Escherichia coli, Y. pseudotuberculosis, Y. enterocolitica , and Pst − Y. pestis . By means of these methods, in conjuntion with fractional precipitation with ammonium sulfate, the antibacterial activity was purified to homogeneity (as judged by disc gel electrophoresis and analysis by double diffusion in agar). The relative sensitivity of each cell type was constant and not affected by the state of purification. These findings indicate that the pesticin molecule alone accounts for lethality. Cells of E. coli and Y. pseudotuberculosis were about 100 and 10 times more sensitive to pesticin, respectively, than were those of Pst − Y. pestis . Activity directed against sensitive cells of these three species was enhanced by 0.1% ethylenediaminetetraacetate (EDTA) and completely inhibited by 0.01 m hemin or 0.001 m Fe 3+ . The response of Y. enterocolitica to pesticin was variable in the presence of EDTA and was not influenced by Fe 3+ . Attempts to detect common surface structures responsible for absorption of pesticin by the four cell types were not successful.

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Congo Red-Agar Plating Medium for Detecting Pigmentation in Pasteurella pestis

Cited by 121 publications

References 5 publications

CsrA Enhances Cyclic-di-GMP Biosynthesis and Yersinia pestis Biofilm Blockage of the Flea Foregut by Alleviating Hfq-Dependent Repression of the hmsT mRNA

CsrA Enhances Cyclic-di-GMP Biosynthesis and Yersinia pestis Biofilm Blockage of the Flea Foregut by Alleviating Hfq-Dependent Repression of the hmsT mRNA

Characterization of the hemin-binding property of Porphyromonas endodontalis

Specificity, Induction, and Absorption of Pesticin

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