Congenital Toxoplasmosis: Evaluation of Molecular and Serological Methods for Achieving Economic and Early Diagnosis Among Egyptian Preterm Infants

Said, Reem N.; Zaki, M.M.; Abdelrazik, Manal B.

doi:10.1093/tropej/fmq097

Cited by 9 publications

(7 citation statements)

References 24 publications

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“…The simultaneous presence of anti- T . gondii IgM and IgG classes is transient in most cases and therefore the percentage of pregnant women with this profile is smaller than the other mentioned above [ 14 , 18 , 31 , 47 , 49 , 50 ].…”

Section: Discussionmentioning

confidence: 88%

Prematurity and Low Birth Weight did not Correlate with Anti-Toxoplasma gondii Maternal Serum Profiles – a Brazilian Report

et al. 2015

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Gestational Toxoplasma gondii infection is considered a major risk factor for miscarriage, prematurity and low birth weight in animals. However, studies focusing on this topic in humans are scarce. The objective of this study is to determine whether anti-Toxoplasma gondii maternal serum profiles correlate prematurity and low birth weight in humans. The study examined 213 pregnant women seen at the High-Risk Pregnancy Hospital de Base, São José do Rio Preto, São Paulo, Brazil. All serological profiles (IgM-/IgG+; IgM-/IgG-; IgM+/IgG+) were determined by ELISA commercial kits. Maternal age, gestational age and weight of the newborn at birth were collected and recorded in the Statement of Live Birth. Prematurity was defined as gestational age <37 weeks and low birth weight ≤ 2499 grams. The t-test was used to compare values (p < 0.05). The mean maternal age was 27.6±6.6 years. Overall, 56.3% (120/213) of the women studied were IgM-/IgG+, 36.2% (77/213) were IgM-/IgG- and 7.5% (16/213) were IgM+/IgG+. The average age of the women with serological profile IgM+/IgG+ (22.3±3.9 years) was different from women with the profile IgM-/IgG+ (27.9±6.7 years, p = 0.0011) and IgM-/IgG- (27.9±6.4 years, p = 0.0012). There was no statistically significant difference between the different serological profiles in relation to prematurity (p = 0.6742) and low birth weight (p = 0.7186). The results showed that prematurity and low birth weight did not correlate with anti-Toxoplasma gondii maternal serum profiles.

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Section: Discussionmentioning

confidence: 88%

Prematurity and Low Birth Weight did not Correlate with Anti-Toxoplasma gondii Maternal Serum Profiles – a Brazilian Report

et al. 2015

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“…However, these data are not enough to predict the time that the infection occurred. This condition seems to be common in patients infected with T. gondii irrespective of clinical evidence of ocular disease [ 25 , 54 , 56 , 61 , 62 ]. The other patients were not positive for IgM and IgA antibodies at inclusion in the study but the identification of IgG antibodies was used to infer a chronic infection.…”

Section: Discussionmentioning

confidence: 99%

A Brazilian report using serological and molecular diagnosis to monitoring acute ocular toxoplasmosis

et al. 2015

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BackgroundToxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging. The aim of this study was to evaluate the use of serology and molecular methods to monitor acute OT in immunocompetent patients during treatment.MethodsFive immunocompetent patients were clinically diagnosed with acute OT. The clinical evaluation was performed by ophthalmologic examination using the Early Treatment Diabetic Retinopathy Study, best-corrected visual acuity, slit lamp biomicroscopy, fundoscopic examination with indirect binocular ophthalmoscopy color fundus photography, fluorescein angiography and spectral optical coherence tomography (OCT). Serology were performed by ELISA (IgA, IgM, IgG) and confirmed by ELFA (IgG, IgM). Molecular diagnoses were performed in peripheral blood by cPCR using the Toxoplasma gondiiB1 gene as the marker. Follow-up exams were performed on day +15 and day +45.ResultsOnly five non-immunocompromised male patients completed the follow up and their data were used for analysis. The mean age was 41.2 ± 11.3 years (median: 35; range 31–54 years). All of them were positive for IgG antibodies but with different profiles for IgM and IgA, as well as PCR. For all patients the OCT exam showed active lesions with the inner retinal layers being abnormally hyper-reflective with full-thickness disorganization of the retinal reflective layers, which assumed a blurred reflective appearance and the retina was thickened.ConclusionsThe presence of IgA and IgM confirmed the acute infection and thus was in agreement with the clinical evaluation. Our results show the adopted treatment modified the serological profile of IgM antibodies and the PCR results, but not the IgG and IgA antibodies and that imaging is a good tool to follow-up patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-015-1650-6) contains supplementary material, which is available to authorized users.

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“…The frequency of preterm newborns included in the individual studies varied from 0.4% (n = 7/1683) to 100% (n = 80/80) (a study that included only preterm cases). 16 Preterm birth was defined in 3 studies as gestational age (in weeks) < 37 14,17 to < 38 15 weeks. The other 3 studies did not define preterm birth using a cut-off but instead included those subjects documented with prematurity (Wang et al 12 and Hasby Saad et al 13 ) or preterm labor (Djurkovic-Djakovic et al 18 ), as reported in medical records (Table 1).…”

Section: Preterm Deliverymentioning

confidence: 99%

A Systematic Review to Evaluate a Possible Association Between Congenital Toxoplasmosis and Preterm Labor

Grubb¹,

Cárdenas

Narasimhan

et al. 2022

Pediatric Infectious Disease Journal

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Background: Congenital toxoplasmosis (CT) is a widespread infection in several countries, and it is defined as an infection of a fetus, newborn, or infant under 1 year of age. Moreover, it represents a thread to pregnant women globally. The objective of our study is to evaluate a potential association between prematurity and CT and whether intrauterine transmission impacts gestational length during pregnancy. Methods: PubMed, Cochrane Library and Google Scholar databases were searched from 1950 to 2019. Case-control studies, retrospective, and prospective cohort studies were eligible. Seven studies were included from a total of 314. The Newcastle-Ottawa scale was used to establish the quality of the articles included. Results: Based on our review, an association between CT and preterm labor was not established, which may reflect heterogeneity in screening, treatments administered, and differing reported incidences of CT across continents over 69 years. A multicenter prospective cohort study powered to investigate a potential association is indicated. Conclusion: Further studies are needed including multicenter prospective cohort studies powered to investigate key clinical associations such as vertical transmission and preterm birth.

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Congenital Toxoplasmosis: Evaluation of Molecular and Serological Methods for Achieving Economic and Early Diagnosis Among Egyptian Preterm Infants

Abstract: Determination of avidity of IgG toxoplasma antibodies and/or serological detection of specific IgA for toxoplasmosis offer, simple tests for diagnosis of congenital toxoplasmosis with (better sensitivity) than IgM.

Cited by 9 publications

References 24 publications

Prematurity and Low Birth Weight did not Correlate with Anti-Toxoplasma gondii Maternal Serum Profiles – a Brazilian Report

Prematurity and Low Birth Weight did not Correlate with Anti-Toxoplasma gondii Maternal Serum Profiles – a Brazilian Report

A Brazilian report using serological and molecular diagnosis to monitoring acute ocular toxoplasmosis

A Systematic Review to Evaluate a Possible Association Between Congenital Toxoplasmosis and Preterm Labor

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