Congelation of cryoprotective solutions and cryopreservation of fish sperm

Andreev, A. N.; Sadikova, D. G.; Гахова, Э. Н.; Пашовкин, Т. Н.; Tikhomirov, A. M.

doi:10.1134/s0006350909050108

Cited by 20 publications

(10 citation statements)

References 11 publications

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“…Although DMSO is a widely used cryoprotectant owing to its fast penetration into spermatozoa and its stabilizing interactions with phospholipids of the sperm plasma membrane (Suquet et al, 2000), egg yolk is an important component of cryopreservation solutions for fish sperm, as its absence or surplus negatively affects the fertilization capability of spermatozoa after freeze thaw (Cherepanov and Kopeika, '99). This effect of egg yolk is likely the result of alterations in the formation and size of ice microparticles during the freezing process, which positively impacts spermatozoid survival during cryopreservation by preventing damage to the plasma membrane (Suquet et al, 2000; Andreev et al, 2009). Importantly, it has been shown that intracellular and extracellular cryoprotectants interact to improve the overall cryoprotection of sperm from marine animals (Suquet et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Amphioxus spawning behavior in an artificial seawater facility

et al. 2011

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Owing to its phylogenetic position at the base of the chordates, the cephalochordate amphioxus is an emerging model system carrying immense significance for understanding the evolution of vertebrate development. One important shortcoming of amphioxus as a model organism has been the unavailability of animal husbandry protocols to maintain amphioxus adults away from the field. Here, we present the first report of successful maintenance and spawning of Branchiostoma lanceolatum adults in a facility run on artificial seawater. B. lanceolatum has been chosen for this study because it is the only amphioxus species that can be induced to spawn. We provide a step-by-step guide for the assembly of such a facility and discuss the day-to-day operations required for successful animal husbandry of B. lanceolatum adults. This work also includes a detailed description of the B. lanceolatum spawning behavior in captivity. Our analysis shows that the induced spawning efficiency is not sex biased, but increases as the natural spawning season progresses. We find that a minor fraction of the animals undergo phases of spontaneous spawning in the tanks and that this behavior is not affected by the treatment used to induce spawning. Moreover, the induced spawning efficiency is not discernibly correlated with spontaneous spawning in the facility. Last, we describe a protocol for long-term cryopreservation of B. lanceolatum sperm. Taken together, this work represents an important step toward further establishing amphioxus as a laboratory animal making it more amenable to experimental research, and hence assists the coming of age of this emerging model.

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Section: Resultsmentioning

confidence: 99%

Amphioxus spawning behavior in an artificial seawater facility

et al. 2011

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“…In one study the investigators observed sperm vitrification in rainbow trout (listed in the article as O. mikiss) and Russian sturgeon Acipenser gueldenstaedtii (listed in the article as A. guldenshtadti) by use of cryomicroscopy. 29 The second study specifically addressed fish sperm vitrification and development of a streamlined protocol for sperm vitrification in channel catfish, 20 and a recent study reported sperm vitrification in rainbow trout without the use of cryoprotectants but no production of offspring was reported. 23 These are all large-bodied (e.g., > 2.5 kg) externally fertilizing species.…”

Section: Sperm Vitrification Of Xiphophorus Helleriimentioning

confidence: 99%

Production of F₁Offspring with Vitrified Sperm from a Live-Bearing Fish, the Green SwordtailXiphophorus hellerii

et al. 2011

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This study reports the first production of offspring with vitrified sperm from a live-bearing fish Xiphophorus hellerii. The overall goal of this study was to develop streamlined protocols for integration into a standardized approach for vitrification of aquatic species germplasm. The objectives were to (1) estimate acute toxicity of cryoprotectants, (2) evaluate vitrification solutions, (3) compare different thawing methods, (4) evaluate membrane integrity of post-thaw sperm vitrified in different cryoprotectants, and (5) evaluate the fertility of vitrified sperm. Nine cryoprotectants and two commercial vitrification additives were tested for acute toxicity and glass forming ability, alone and in combination. Two vitrification solutions, 40% glycerol (Gly) and 20% Gly + 20% ethylene glycol (EG) in 500 mOsmol/kg Hanks' balanced salt solution (HBSS), were selected for vitrification of 10 lL sperm samples using inoculating loops plunged into liquid nitrogen. Samples were thawed at 24°C (one loop in 5 lL of HBSS or three loops in 500 lL of HBSS). Samples thawed in 500 lL were concentrated by centrifugation (1000 g for 5 min at 4°C) into 5 lL for artificial insemination. Offspring were produced from virgin females inseminated with sperm vitrified with 20% Gly + 20% EG and concentrated by centrifugation.

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“…; Hagedorn & Kleinhans ). Recently, sperm vitrification was applied successfully in freshwater fish, and offspring were produced from vitrified sperm samples of Russian sturgeon ( Acipenser gueldenstaedtii , Brandt & Ratzeburg) (Andreev, Sadikova, Gakhova, Pashovkin & Tikhomirov ), channel catfish ( Ictalurus punctatus , Rafinesque) (Cuevas‐Uribe, Leibo, Daly & Tiersch ), green swordtail ( Xiphophorus hellerii , Heckel) (Cuevas‐Uribe, Yang, Daly, Savage, Walter & Tiersch ) and rainbow trout ( Oncorhynchus mykiss , Walbaum) (Figueroa, Risopatron, Sanchez, Isachenko, Merino, Isachenko & Valdebenito ). Despite this, sperm vitrification remains unexplored in marine fish.…”

Section: Introductionmentioning

confidence: 99%

Vitrification of sperm from marine fish: effect on motility and membrane integrity

et al. 2013

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Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (VS); 3) evaluate different thawing solutions, and 4) evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper (Lutjanus campechanus), spotted seatrout (Cynoscion nebulosus), and red drum (Sciaenops ocellatus). A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Samples were loaded onto 10-µL polystyrene loops and plunged into liquid nitrogen. There was a significant difference (P < 0.05) in post-thaw motility among VS and among species when using the same VS. The sperm in VS of 15% DMSO + 15% ethylene glycol + 10% glycerol + 1% X-1000™ + 1% Z-1000™ had an average post-thaw motility of 58% and membrane integrity of 19% for spotted seatrout, 38% and 9% for red snapper, and 30% and 19% for red drum. Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations. Vitrification offers an alternative to conventional cryopreservation.

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Congelation of cryoprotective solutions and cryopreservation of fish sperm

Cited by 20 publications

References 11 publications

Amphioxus spawning behavior in an artificial seawater facility

Amphioxus spawning behavior in an artificial seawater facility

Production of F₁Offspring with Vitrified Sperm from a Live-Bearing Fish, the Green SwordtailXiphophorus hellerii

Vitrification of sperm from marine fish: effect on motility and membrane integrity

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Congelation of cryoprotective solutions and cryopreservation of fish sperm

Cited by 20 publications

References 11 publications

Amphioxus spawning behavior in an artificial seawater facility

Amphioxus spawning behavior in an artificial seawater facility

Production of F1Offspring with Vitrified Sperm from a Live-Bearing Fish, the Green SwordtailXiphophorus hellerii

Vitrification of sperm from marine fish: effect on motility and membrane integrity

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Production of F₁Offspring with Vitrified Sperm from a Live-Bearing Fish, the Green SwordtailXiphophorus hellerii