Abstract:Isolation of glomeruli was done by microdissection. Tubuli were isolated after "dissociative" treatment with collagenase, according to Guder. (Hoppe-Seyler's 2. PhysioI. Chemie 352,1319Chemie 352, 1971. We observed a linear dose dependency of enzyme in microdissected glomeruli and in isolated tubuli. Enzyme activity of 80 glomeruli was the lowest measurable. 90% in inhibition of Kinin formation was observed by the use of Trasylol. Carboxypeptidase B completey destroyed the uterus contracting material. There w… Show more
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