Conformational study of a new SGTx1 neurotoxin from the spider <i>Scodra griseipes</i> using mass spectrometry

Marvin, Laure; Lange, Catherine

doi:10.1002/rcm.261

Cited by 3 publications

(9 citation statements)

References 45 publications

(82 reference statements)

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“…Thus, we have shown here a dramatic difference in CSD between disulfideintact and disulfide-reduced peptides. Such differences have already been shown for ω-conotoxins 36 and SGTx1 toxin, 37 which contain only 26 and 34 amino acids, respectively. An alternative explanation could involve Coulombic forces at acidic pH in solution.…”

Section: Native α-Dendrotoxinmentioning

confidence: 60%

“…Optimization of the parameters was obtained for water + methanol (80 / 20,v : v) at pH 7. 36,37 The ES ionization source temperature was also optimised at 100°C. In fact, at 80°C the spectrum was much too noisy and there was no difference in the CSD, centered at 8+, between these two temperatures.…”

Section: Mass Spectrometry Instrumentationmentioning

confidence: 99%

“…The extraction cone voltage was chosen at a middle value of 150 V. A high value of this voltage (250 V) shifts the envelope of the multiply-charged ions towards a low CSD (5+), with a much better transmission, in contrast to a low value of extraction cone voltage (50 V), which shifts the ions to a higher CSD (7+). 36,37…”

Section: Mass Spectrometry Instrumentationmentioning

confidence: 99%

“…[4][5][6][7][8][9] So, examination of CSD in positive-ion ES might be a good way to measure the number of charges corresponding to the number of ionized basic residues. Nevertheless, it has been shown that the observation of CSD might be very different 36,37 when the structure presents multiple disulfide bridges and a large number of basic amino acids. α-Dendrotoxin is a 59-amino-acid, three-disulfide-bridged, peptide (calculated average mass 7048.1 Da), including 15 basic residues (eight Arg, six Lys and one His).…”

Section: Csd Of Native α-Dendrotoxinmentioning

confidence: 99%

“…7,33 H/D exchanges are slow when the hydrogen of an amino acid residue is either buried in an hydrophobic core or involved in hydrogen bonding, such as α-helices or β-sheets, or the formation of ionic linkages. Such H/D exchange studies have not been realized with multiple disulfide-bridged proteins, except for proinsulin, 34 pancreatic trypsin inhibitor, 34 ribonuclease, 31,32 lysozyme, 35 conotoxin 36 and a new SGTx1 toxin of the venom of Scodra griseipes tarantula 37 (three and four disulfide bridges).…”

Section: Introductionmentioning

confidence: 99%

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Conformational Properties of α-Dendrotoxin Using Electrospray Mass Spectrometry

Belva

Lange

Ségalas

2001

Eur J Mass Spectrom (Chichester)

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To examine the conformation of α-dendrotoxin, charge state distributions (CSD)and hydrogen/deuterium (H/D)isotopic exchange using electrospray-ionization mass spectrometry [ES-MS] have been used. Here, it cannot be deduced from the CSDs that some basic residues are hidden in the core of the structure. At neutral pH, the CSDs reflect the equilibrium bulk solution exactly. Seven basic residues seem to be shielded from the solvent, according to calculations from the X-ray structure. But, under acidic conditions, the CSDs observed in mass spectra are not a true reflection of the equilibrium bulk solution, partly because of an increased intramolecular electrostatic effect with the gaseous ions. H/D exchange experiments show that three to seven hydrogens are involved in hydrogen bonds. The resistance to urea denaturation has also been investigated. Urea and 1,4-dithiothreitol (DTT)denaturation together lead to unfolded conformers. During this reduction of the bridges, it is observed that the Cys16–Cys40 and Cys32–Cys53 linkages are more accessible to the reducing agent than the Cys7–Cys57 linkage, which is very important by maintaining a compact fold.

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Section: Native α-Dendrotoxinmentioning

confidence: 60%

Section: Mass Spectrometry Instrumentationmentioning

confidence: 99%

Section: Mass Spectrometry Instrumentationmentioning

confidence: 99%

Section: Csd Of Native α-Dendrotoxinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conformational Properties of α-Dendrotoxin Using Electrospray Mass Spectrometry

Belva

Lange

Ségalas

2001

Eur J Mass Spectrom (Chichester)

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Current Awareness

2001

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 13th. June. 2001)

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Semiconducting Ta₃N₅-Polymer Nanohybrid that Photoelectrolyze Water

et al. 2014

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The successful preparation of a nanohybrid electrode made from Ta 3 N 5 nanoparticle powders interlinked by Nafion is described. The basic photoelectrochemical properties as anode for water photolysis are investigated and gas evolution as well as corrosion reactions are determined by differential electrochemical mass spectroscopy (DEMS) and X-ray photoelectron spectroscopy (XPS). The sample shows oxygen evolution but also photocorrosion, oxidation of the Ta 3 N 5 nanoparticles via the O 1s line in XPS experiment. The photocorrosion reaction results in a core-shell structure consisting of a tantalum oxide shell and a tantalum nitride core as seen in XPS and x-ray diffraction.In the search for structures and devices that split water with visible light in a Z-scheme analogue of photosynthesis, photonic absorbers that have a suited energy gap and the according absolute positions of the energy bands (1) have still to be identified. More robust systems can be developed using inorganic material components for tandem devices that split water without external bias and with visible light (2). The highest theoretical efficiency is about 44% (3) and, using photocathode materials such as Si or layer transition metal dichalcogenides (4 , 5) which develop between 0.6V and 0.7V photovoltage, the energy gap of the anode material should be in the range of 1.7-1.9eV, thereby providing the additional photovoltage for water splitting (including overpotential losses) of 1.0V-1.2V. The latter assumption is based on the observation that in most semiconductor solar cell structures, the attainable photovoltage does not exceed 60%-70% of the energy gap (6, 7).In the development of photoanodes with Earth abundant materials and using scalable processes, only a few candidates have been identified and studied for the oxidative lightinduced water dissociation. They comprise WO 3 , BiVO 4 and Fe 2 O 3 , for example (8,9). Although limited by too large energy gaps or an unfavorable position of the band edges with respect to the water oxidation potential, the materials are intensively investigated (10). Their preparation is typically based on low-cost scalable processes such as electrodeposition, sol-gel synthesis, spin coating and spray pyrolysis (11). Besides combinatorial approaches to identify novel photoanode materials (10), the exploration and extension of preparation methods represents another route to develop materials and material composites.In this work, we present a method that allows the preparation of nanocomposite materials from powder samples that can be used as photoactive electrodes. The method is generally applicable for powder samples and will allow the screening of a large range of nanoparticle samples that have so far been unaccessible for inclusion in the search for 10.1149/05830.0001ecst ©The Electrochemical Society ECS Transactions, 58 (30) 1-7 (2014) 1 ) unless CC License in place (see abstract). ecsdl.org/site/terms_use address. Redistribution subject to ECS terms of use (see 137.99.31.134 Downloaded on 2015-04-12 to IP

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Conformational study of a new SGTx1 neurotoxin from the spider Scodra griseipes using mass spectrometry

Cited by 3 publications

References 45 publications

Conformational Properties of α-Dendrotoxin Using Electrospray Mass Spectrometry

Conformational Properties of α-Dendrotoxin Using Electrospray Mass Spectrometry

Current Awareness

Semiconducting Ta₃N₅-Polymer Nanohybrid that Photoelectrolyze Water

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Conformational study of a new SGTx1 neurotoxin from the spider Scodra griseipes using mass spectrometry

Cited by 3 publications

References 45 publications

Conformational Properties of α-Dendrotoxin Using Electrospray Mass Spectrometry

Conformational Properties of α-Dendrotoxin Using Electrospray Mass Spectrometry

Current Awareness

Semiconducting Ta3N5-Polymer Nanohybrid that Photoelectrolyze Water

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Semiconducting Ta₃N₅-Polymer Nanohybrid that Photoelectrolyze Water