Conformational studies of immunoglobulin G and its subunits by the methods of hydrogen deuterium exchange and infrared spectroscopy

Abaturov, L. V.; Nezlin, Roald; Vengerova, T.I.; Varshavsky, Ja.M.

doi:10.1016/0005-2795(69)90099-3

Cited by 35 publications

(15 citation statements)

References 26 publications

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“…In the present study, the antigen binding activity of human IgG monomers survived acid treatment at pH 3.0-3.5 but the complement binding activity was reduced by the treatment. This phenomenon may correlate with the phenomenon that Fab and F(ab' )2 fragments were more stable than Fc fragment in acidic condition (Abaturov et al 1969;Charlwood and Utsumi 1969).…”

Section: Discussionmentioning

confidence: 66%

Dissociation of aggregated IgG and denaturation of monomeric IgG by acid treatment.

Uemura¹

1983

Tohoku J. Exp. Med.

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Section: Discussionmentioning

confidence: 66%

Dissociation of aggregated IgG and denaturation of monomeric IgG by acid treatment.

Uemura¹

1983

Tohoku J. Exp. Med.

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“…Abaturov L. V. et al [54] have reported the observed frequencies of four amide bands of IgG, its peptide chains and proteolytic fragments. The frequencies and the shape of the amide bands of sulfonated IgG and F(ab') 2 fragment were identical to those of the complete molecule.…”

Section: Discussionmentioning

confidence: 99%

Vibrational Studies of Different Human Body Disorders Using FTIR Spectroscopy

Kumar¹,

Reena²,

Chaudhary³

et al. 2014

OJAppS

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Some of the important features of the bands occur in the present study. The band called amide A is available in all the diseased and healthy controls and the frequency of the band ranges from 3380 cm −1 to 3480.74 cm −1 . The band due to C-H band and called hydrocarbon band was found only in paralytic and Alzheimer diseased along with normal healthy controls. Carbide band (C=C) is found only in Duchenne muscular dystrophy, paralytic and Alzheimer's disease patients. Amide I was intact in all disorders with normal persons. Peroxide band (O-O) was found in all the cases of study. Amide IV band was found in paralytic, muscular dystrophy, Alzheimer's diseases and normal controls. The amide V band was found in Alzheimer's diseases only. The appearance or disappearance of the bands is a good sign to understand the mechanisms at the molecular level. FTIR spectroscopy may help in the diagnosis of the disease at the early stage of the onset. This spectroscopy can be used nicely for the study of hair, vaginal fluid, nails, urine, mucus, semen, synovial fluid, blood, hemoproteins, skin, and tears for human beings. We can also use it to understand the effect of adulteration on food and paint technology. FTIR is an indicator to explore the changes occurring at molecular level.

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“…The rate of hydrogen -deuterium (' H + 2H) exchange in peptide NH groups was monitored by infrared spectroscopy [8] with the use of UR-10 and UR-20 spectrophotometers (Carl Zeiss, Iena, DDR). The exchange reaction was initiated by gel filtration through a short column of Sephadex G-25 (fine), equilibrated with a heavy water buffer.…”

Section: Methodsmentioning

confidence: 99%

Effect of inter‐subunit contact on intramolecular conformational motility (conformational stability) of hemoglobin as revealed by hydrogen exchange

Abaturov¹,

Yakobashvily²,

Jinoria³

et al. 1976

FEBS Letters

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Conformational studies of immunoglobulin G and its subunits by the methods of hydrogen deuterium exchange and infrared spectroscopy

Cited by 35 publications

References 26 publications

Dissociation of aggregated IgG and denaturation of monomeric IgG by acid treatment.

Dissociation of aggregated IgG and denaturation of monomeric IgG by acid treatment.

Vibrational Studies of Different Human Body Disorders Using FTIR Spectroscopy

Effect of inter‐subunit contact on intramolecular conformational motility (conformational stability) of hemoglobin as revealed by hydrogen exchange

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