2009
DOI: 10.1002/bip.21295
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Conformational properties of angiotensin II and its active and inactive TOAC‐labeled analogs in the presence of micelles. Electron paramagnetic resonance, fluorescence, and circular dichroism studies

Abstract: The interaction between angiotensin II (AII, DRVYIHPF) and its analogs carrying 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) and detergents--negatively charged sodium dodecyl sulfate (SDS) and zwitterionic N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (HPS)--was examined by means of EPR, CD, and fluorescence. EPR spectra of partially active TOAC1-AII and inactive TOAC3-AII in aqueous solution indicated fast tumbling, the freedom of motion being greater at the N-terminus. Line b… Show more

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Cited by 13 publications
(11 citation statements)
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“…The small k max blue shift observed when Labaditin is in contact with LPC points out that, probably, both forms of the peptide are not so embedded in the acyl-chain region of LPC micelles. The values obtained were quite low when compared with others peptides as Hylin-a1, for instance, that showed about 25 nm of shift [ [50][51][52]. Such finding suggests that the peptide must be located near the LPC micelle surface.…”
Section: Fluorescence and CD Resultsmentioning
confidence: 68%
“…The small k max blue shift observed when Labaditin is in contact with LPC points out that, probably, both forms of the peptide are not so embedded in the acyl-chain region of LPC micelles. The values obtained were quite low when compared with others peptides as Hylin-a1, for instance, that showed about 25 nm of shift [ [50][51][52]. Such finding suggests that the peptide must be located near the LPC micelle surface.…”
Section: Fluorescence and CD Resultsmentioning
confidence: 68%
“…Combined EPR and CD studies were also performed on the interaction of BK (Vieira et al 2002) and AII (Vieira et al 2009) and their TOAC-labeled analogues with detergent micelles—negatively charged SDS and zwitterionic HPS. Line broadening in the EPR spectra indicated micelle binding (Fig.…”
Section: Biologically Active Peptides: Ligands and Fragments Of G Promentioning
confidence: 99%
“…The arrows in the spectrum of TOAC 3 -AII in the presence of HPS at pH 10.0 indicate an immobilized component due to HPS-bound TOAC 3 -AII. Reprinted from Vieira et al (2009) with permission of John Wiley & Sons…”
Section: Biologically Active Peptides: Ligands and Fragments Of G Promentioning
confidence: 99%
“…Small linear peptides usually exist in solution as a range of conformations in rapid equilibrium (Matsoukas et al, 1990). Accordingly, interpretation of the conformational studies of AngII are conflicting, which has led to various structural models in the solution including β pleated sheet, β and γ turns, and other structures (Carpenter, Wilkes, & Schiller, 1998;Greff et al, 1976;Lintner et al, 1977;Spyroulias et al, 2003;Tzakos et al, 2004;Vieira et al, 2009). One reason for contradicting results could possibly be due to solvent effects.…”
Section: Discussionmentioning
confidence: 99%