Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation

Lee, Soon Goo; Jez, Joseph M.

doi:10.1074/jbc.ra117.000106

Cited by 16 publications

(31 citation statements)

References 50 publications

(80 reference statements)

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“…PMT1 has previously been shown to complement the Δ cho2 Δ opi3 mutant under the same conditions (Bolognese and McGraw, ). Therefore, our results suggest that both PMT1 and PMT3 may be capable of catalyzing all three methylation steps in vivo , in agreement with the results of a recent in vitro enzyme study on PMT3 (Lee and Jez, ). As the first methylation of PEtn is crucial for the production of choline moieties (Hanson and Rhodes, ; Datko and Mudd, ,b; Summers and Weretilnyk, ; Nuccio et al ., ) (Figure b), the double PMT1 and PMT3 knockout with an affected first methylation step may have changes to PCho production that would influence Cho and PtdCho levels (Figure ).…”

Section: Discussionsupporting

confidence: 92%

“…Disruption of PMT1 by T‐DNA caused growth defects specifically in roots but has little effect on PCho levels (Cruz‐Ramírez et al ., ). Mutation in PMT2 and PMT3 had no effect on the plant growth (BeGora et al ., ; Lee and Jez, ). Therefore, although these results suggested that these three PMT isoforms may have redundant function in PCho biosynthesis, multiple gene knock‐out studies have not yet been reported.…”

Section: Introductionmentioning

confidence: 98%

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A pair of phospho‐base methyltransferases important for phosphatidylcholine biosynthesis in Arabidopsis

et al. 2018

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Phosphatidylcholine (PtdCho) is a predominant membrane lipid class in eukaryotes. Phospho-base N-methyltransferase (PMT) catalyzes a critical step in PtdCho biosynthesis. However, in Arabidopsis thaliana, the discovery of involvement of the specific PMT isoform in PtdCho biosynthesis remains elusive. Here, we show that PMT1 and PMT3 redundantly play an essential role in phosphocholine (PCho) biosynthesis, a prerequisite for PtdCho production. A pmt1 pmt3 double mutant was devoid of PCho, which affected PtdCho biosynthesis in vivo, showing severe growth defects in post-embryonic development. PMT1 and PMT3 were both highly expressed in the vasculature. The pmt1 pmt3 mutants had specifically affected leaf vein development and showed pale-green seedlings that were rescued by exogenous supplementation of PCho. We suggest that PMT1 and PMT3 are the primary enzymes for PCho biosynthesis and are involved in PtdCho biosynthesis and vascular development in Arabidopsis seedlings.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 98%

A pair of phospho‐base methyltransferases important for phosphatidylcholine biosynthesis in Arabidopsis

et al. 2018

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“…and was most abundant in leaves, whether juvenile or adult, expanding or mature, rosette 293 leaves or cauline leaves, while NMT1 was hardly expressed there, consistent with available 294 transcriptomic data , Zimmermann et al, 2004Lee and Jez, 2017 …”

supporting

confidence: 60%

“…Contrary to an earlier report (BeGora et al 2010), a recent enzymatic study concluded to the 781 in vitro capacity of NMT2 to catalyse the conversion of PEA to PCho (Jez et al 2017).…”

contrasting

confidence: 43%

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NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

et al. 2018

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54Phosphatidylcholine (PC) is a major membrane phospholipid and a precursor for most 55 signalling molecules. Understanding its synthesis is important for improving plant growth, 56 nutritional value and resistance to stress. PC synthesis is complex, involving several 57 interconnected pathways, one of which proceeds from serine-derived phosphoethanolamine 58(PEA) to form phosphocholine (PCho) through three sequential phospho-base methylations 59 catalysed by phosphoethanolamine N-methyltransferases (PEAMTs). The contribution of 60 this pathway to the production of PC and plant growth has been a matter of some debate.

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Functional analysis of phosphoethanolamine N-methyltransferase in plants and parasites: Essential S-adenosylmethionine-dependent methyltransferase in choline and phospholipid metabolism

Bare¹,

Thomas²,

Etoroma³

et al. 2023

Biochemical Pathways and Environmental Responses in Plants: Part B

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Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation

Cited by 16 publications

References 50 publications

A pair of phospho‐base methyltransferases important for phosphatidylcholine biosynthesis in Arabidopsis

A pair of phospho‐base methyltransferases important for phosphatidylcholine biosynthesis in Arabidopsis

NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

Functional analysis of phosphoethanolamine N-methyltransferase in plants and parasites: Essential S-adenosylmethionine-dependent methyltransferase in choline and phospholipid metabolism

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