Conformational change of single-stranded RNAs induced by liposome binding

Abstract

The interaction between single-stranded RNAs and liposomes was studied using UV, Fourier Transform Infrared spectroscopy (FTIR) and Circular Dichroism spectroscopy (CD). The effect of the surface characteristics of liposomes, which were composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and modified with cholesterol (Ch) or 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), on the liposome–RNA interaction was investigated. The fluorescence of 6-(p-toluidino)naphthalene-2-sulfonate (TNS) embedded in the liposome surface (ε = 30–40) was decreased in the presence of tRNA, suggesting that single-stranded tRNA could bind onto the liposome. The dehydration of –PO2− –, guanine (G) and cytosine (C) of tRNA molecules in the presence of liposomes suggested both an electrostatic interaction (phosphate backbone of tRNA and trimethylammonium group of POPC, DOTAP) and a hydrophobic interaction (guanine or cytosine of tRNA and aliphatic tail of lipid). The tRNA conformation on the liposome was determined by CD spectroscopy. POPC/Ch (70/30) maintained tRNA conformation without any denaturation, while POPC/DOTAP(70/30) drastically denatured it. The mRNA translation was evaluated in an Escherichia coli cell-free translation system. POPC/Ch(70/30) enhanced expression of green fluorescent protein (GFP) (116%) while POPC/DOTAP(70/30) inhibited (37%), suggesting that the conformation of RNAs was closely related to the translation efficiency. Therefore, single-stranded RNAs could bind to liposomal membranes through electrostatic and hydrophobic attraction, after which conformational changes were induced depending on the liposome characteristics.

Pseudo-Interphase of Liposome Promotes 1,3-Dipolar Cycloaddition Reaction of Benzonitrile Oxide and N-Ethylmaleimide in Aqueous Solution

“…The ΔGP 340 value of DOTAP was highest among the liposomes used in this study, although the change in the GP 340 value of DOTAP was not as significant as compared to the phase transition from the liquiddisordered phase to the solid-ordered phase. 29 BNO could be embedded in the hydrophobic−hydrophilic interface region where water molecules bind, and could replace the water molecules, resulting in the dehydration of the membrane The Journal of Physical Chemistry B Article surface (i.e., an increase in the GP 340 value). Here, a few percent of water was excluded from the liposome membrane (the relative dielectric constant ε′ varied to 25.0 from 26.3; see below).…”

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

“…The interaction mechanism of liposomes with biomacromolecules has been recently investigated [42,48], focusing on the recognition, folding, and functionalization by selecting single-stranded RNA molecules as targets ( Figure 6). Liposome surfaces were modified with Ch, DOTAP, and DC-Ch, and the physicochemical properties of the liposomes were analyzed by using the fluorescent probes, DPH and …”

“…It was shown that the liposomes affected the conformation of single-stranded RNAs [42,[45][46][47][48], which is a key parameter affecting their functions [53][54][55]. The dependence of the liposome-RNA interaction on the phase state of the CLs is also discussed.…”

“…A liposome membrane is effective in its use as a platform to perform the above purpose because it looks after the fundamental characteristics of a conventional extraction system and the recognition surface of the biomolecule ( Figure 1). In practice, there are many reports on the possible role of a liposome membrane to recognize various molecules , such as asymmetric recognition [56-57], metal affinity [4], peptide recognition [7,58], enzyme-like function [8,59], RNA recognition [6,42,48], and others [9,28] (Figure 8). membrane modules [61], and hydrogel immobilization of the liposomes at high concentration ( Figure 9) [62].…”

Characterization of the physicochemical properties of phospholipid vesicles prepared in CO2/water systems at high pressure

“…Emergent properties arising from the membrane of the liposome (vesicle) have been introduced, where the vesicle (liposome) membrane can selectively interact with various types of molecules under particular conditions. 12 The important functions of vesicles include (1) interaction with proteins (enzymes) and nucleotides, [13][14][15][16][17] (2) storage of encapsulated molecules, 1,18 and (3) fusion with membranes. 19 Vesicle membranes are dynamic, for example, vesicles composed of 1,2-dimylistoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) possess phase transition temperatures (T m ) at 23.6 and 41.…”