Conformational analysis of galactomannans: from oligomeric segments to polymeric chains

Petkowicz, Carmen Lúcia de Oliveira; Reicher, Fany; Mazeau, K.

doi:10.1016/s0144-8617(98)00051-4

Cited by 70 publications

(48 citation statements)

References 36 publications

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“…We can see that the persistence lengths ( Table 2) of ~ 8 -9 nm which is in good agreement with the results from the Hearst, Bohdanecky and Yamakawa-Fujii approaches in our previous analysis (Picout et al, 2001;2002;Patel et al, 2006) and with computer modelled data for hypothetical galactomannan molecules of ~ 8 -12 nm (Petkowitz, Reicher & Mazeau, 1998). ranging from blue (∆ = 0.13) to red (∆ = 1.00).…”

Section: Fixed Diameter D and Mass Per Unit Length M Lsupporting

confidence: 89%

“…ranging from blue (∆ = 0.13) to red (∆ = 1.00). We can see that persistence lengths (Table 3) are in the range 7 -10 nm and in good agreement with those calculated when the mass per unit length was fixed and with the results from the Hearst, Bohdanecky and Yamakawa-Fujii approaches in our previous analyses (Picout et al, 2001;2002;Patel et al, 2006) and with the computer modelled data of Petkowitz et al (1998).…”

Section: Fixed Diameter D and Mass Per Unit Length M Lsupporting

confidence: 86%

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Global hydrodynamic analysis of the molecular flexibility of galactomannans

Morris

Patel

Picout

et al. 2008

Carbohydrate Polymers

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In the past, intrinsic viscosity and sedimentation velocity analyses have been used separately to assess the conformation and flexibility of guar and locust bean gum galactomannans based on worm-like chain and semi-flexible coil models. Publication of a new global method combining data sets of both intrinsic viscosity and sedimentation coefficient with molecular weight, and minimising a target (error) function now permits a more robust analysis. Using this approach, values for the persistence length of (10 ± 2) nm for guar and (7 ± 1) nm for locust bean gum are returned if the mass per unit length M L is floated as a variable. Using a fixed mass per unit length based on the known compositional data of each galactomannan yields a similar value for L p in both cases, (8 ± 1) nm for guar and (9 ± 1) nm for locust bean gum, with combined set of data yielding (9 ± 1) nm: within experimental error the flexibilities of both galactomannans are very similar.

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Section: Fixed Diameter D and Mass Per Unit Length M Lsupporting

confidence: 89%

Section: Fixed Diameter D and Mass Per Unit Length M Lsupporting

confidence: 86%

Global hydrodynamic analysis of the molecular flexibility of galactomannans

Morris

Patel

Picout

et al. 2008

Carbohydrate Polymers

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“…20 However, in that work instead of minimum B', a different minimum within the main φ trough was reported to occur. MM2CARB calculations have also conducted to a similar qualitative pattern, 18 with the same five minima as the present paper, but lower relative energies for the lateral minima B' and D, as expected from a method which gave less weight to the exoanomeric effect than MM3.…”

Section: Introductionmentioning

confidence: 77%

MM3 Potential energy surfaces of β-4-linked mannobiose and mannotriose at different dielectric constants

Stortz¹

2005

Arkivoc

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The adiabatic potential energy surface (PES) of β-4-linked mannobiose was obtained using the MM3 force field at ε=3 and ε=80, and plotted as contour maps and as 2D graphs representing the energy vs. the ψ angle. The surfaces of the corresponding trisaccharide were also obtained and represented by a single 3D contour map for which the energy is plotted against the two ψ glycosidic angles. The PES of the disaccharide contains a low-energy well comprising two different minima, and three more minima in different locations. No major change was observed by changing the dielectric constant. For the trisaccharide, four main minima were observed, located within one minimum-energy region. The minima have a geometry close to that experimentally obtained for mannobiose, mannotriose and mannan I in solid state, but differ from that expected in aqueous solutions. The flexibility of the glycosidic linkage increases at higher dielectric constant, whereas it decreases for the linkage closer to the reducing end when passing from the di-to the trisaccharide.

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“…Computational simulations suggest that the M/G ratio is a key factor also for PGMs conformational properties [25][26][27]. Galactose substitutions promote chain bending via intramolecular H-bonding, favoring compact conformations and affecting structural properties like persistence length and gyration radius.…”

Section: Introductionmentioning

confidence: 99%

“…Other factors, such as the pattern of galactose substitutions, have also been suggested to affect the conformational properties of PGMs [25,27]. This "fine structure" of the polymers seems to vary even among variants of the same species [28].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Sugar Epimers in Polygalactomannans by ESI-MS/MS

2015

J Anal Bioanal Tech

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Conformational analysis of galactomannans: from oligomeric segments to polymeric chains

Cited by 70 publications

References 36 publications

Global hydrodynamic analysis of the molecular flexibility of galactomannans

Global hydrodynamic analysis of the molecular flexibility of galactomannans

MM3 Potential energy surfaces of β-4-linked mannobiose and mannotriose at different dielectric constants

Quantification of Sugar Epimers in Polygalactomannans by ESI-MS/MS

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